FastQCFastQC Report
Thu 16 Jun 2022
EGAF00006143528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006143528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2406752
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC1555136.461529895892888TruSeq Adapter, Index 11 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCGCGTATGC675462.806520987621491TruSeq Adapter, Index 11 (98% over 50bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACGGCTACATCTCGTATGC47410.1969874752363351TruSeq Adapter, Index 11 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACGGCTACATCTCGTATGC46580.19353884405206684TruSeq Adapter, Index 11 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACGGCTACATCTCGTATGC45050.18718172873648803TruSeq Adapter, Index 11 (98% over 50bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACGGCTACATCTCGTATGC44090.18319295049926207TruSeq Adapter, Index 11 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACGGCTACATCTCGTATGC37100.1541496589594607TruSeq Adapter, Index 11 (98% over 50bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC32540.1352029623326375TruSeq Adapter, Index 11 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)