FastQCFastQC Report
Sun 5 Jun 2022
EGAF00006143530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006143530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11661594
Sequences flagged as poor quality0
Sequence length35-75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA7930946.800905605185706TruSeq Adapter, Index 2 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAC1617781.387271757188597TruSeq Adapter, Index 2 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT1312741.1256951665441277TruSeq Adapter, Index 2 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAT1144560.9814781752820412TruSeq Adapter, Index 2 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATT931030.7983728467995027TruSeq Adapter, Index 2 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA401650.3444211829017543TruSeq Adapter, Index 2 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC371230.31833555515652495TruSeq Adapter, Index 2 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA334290.2866589250148822TruSeq Adapter, Index 2 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGT196640.16862188822557192TruSeq Adapter, Index 2 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA162660.13948350457064446TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA149120.127872741925332TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATG146160.12533449543861672TruSeq Adapter, Index 2 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAACAC144860.12421972502215392TruSeq Adapter, Index 2 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA133430.11441832051433105TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA131790.113011994758178TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA131110.11242888407879746TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA130730.11210302811090833TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA130210.11165711994432322TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA122770.10527720309933618TruSeq Adapter, Index 2 (98% over 63bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)