FastQCFastQC Report
Thu 16 Jun 2022
EGAF00006143536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006143536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4334811
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC1839844.244337296366554TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATGC938442.164892540874331TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTTTGC64330.1484032406487849TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACTTAGGCATCTCGTATGC53370.12311955469338802TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACTTAGGCATCTCGTATGC50780.1171446690524685TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACTTAGGCATCTCGTATGC50710.11698318565676798TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC46100.10634835059706178TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACTTAGGCATCTCGTATGC43460.10025811967349903TruSeq Adapter, Index 3 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)