FastQCFastQC Report
Mon 6 Jun 2022
EGAF00006143538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006143538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2876914
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGTTGG363591.2638194954732744TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGTTGG246610.857203239304338TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGTTGC193950.6741598810391969TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTTTGG173060.6015473524756041TruSeq Adapter, Index 8 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTTTGC165240.5743654485326986TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGTTTG156240.5430819273707869TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGTTGC155370.5400578536584688TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGGTGG135530.4710950692304323TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTTTTG111530.38767234613200113TruSeq Adapter, Index 8 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGGTGG97220.3379315474845616TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC82870.2880517109652913TruSeq Adapter, Index 8 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTTTGC82170.28561854820825366TruSeq Adapter, Index 8 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTTTTC70580.24533232484530298TruSeq Adapter, Index 8 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTTTGG70240.24415050293474186TruSeq Adapter, Index 8 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGATGC63920.22218251918548834TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGGTGC59320.20619316392495568TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGTTTC56900.1977813726791972TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGTTTG53970.1875968485675971TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGATGC49670.17265027734579483TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATGC36360.1263854254941232TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGATGG36350.12635066602616554TruSeq Adapter, Index 8 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGGTGC35930.12489076837194299TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGG31870.11077842438112506TruSeq Adapter, Index 8 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATTC31360.1090056915152834TruSeq Adapter, Index 8 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)