FastQCFastQC Report
Thu 16 Jun 2022
EGAF00006143540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006143540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10632495
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGTTGG296610.2789655673480213TruSeq Adapter, Index 4 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTTTGC207680.19532574433376174TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGTTGC201270.1892970558650627TruSeq Adapter, Index 4 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGGTTGG169060.15900313143810554TruSeq Adapter, Index 4 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC163170.15346350974065823TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTTTGG159280.14980491408648675TruSeq Adapter, Index 4 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGTTTGC119770.11264524460157282TruSeq Adapter, Index 4 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGGTTGC113460.10671060743503759TruSeq Adapter, Index 4 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGGTGG109790.10325892464562644TruSeq Adapter, Index 4 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGTTTG109740.10321189899454455TruSeq Adapter, Index 4 (97% over 45bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)