FastQCFastQC Report
Thu 16 Jun 2022
EGAF00006143574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006143574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4381828
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC3736698.527696659932795TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATGC1983604.526877823593258TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTTTGC101510.23166130665101414TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCAGATCATCTCGTATGC98220.22415302471936369TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCAGATCATCTCGTATGC97600.22273809013042045TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCAGATCATCTCGTATGC90080.20557630285807657TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCAGATCATCTCGTATGC82970.1893501981364855TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCAGATCATCTCGTATGC82520.18832322948322022TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATTC62720.14313660873954887TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCAGATCATCTCGTATGC62300.142178104663168TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTTTGC59160.1350121456159393TruSeq Adapter, Index 7 (97% over 46bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC58690.13393953391141777TruSeq Adapter, Index 7 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)