FastQCFastQC Report
Wed 1 Jun 2022
EGAF00006143576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006143576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9479807
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC5935406.261097931635106TruSeq Adapter, Index 6 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCGCGTATGC2331612.459554292613763TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACGCCAATATCTCGTATGC162910.17184949018476853TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACGCCAATATCTCGTATGC153280.1616910555246536TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACGCCAATATCTCGTATGC151280.159581307931691TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACGCCAATATCTCGTATGC137640.14519282934768607TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACGCCAATATCTCGTATGC136470.14395862700580297TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC106080.11190101233073627TruSeq Adapter, Index 6 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)