FastQCFastQC Report
Thu 16 Jun 2022
EGAF00006143586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006143586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4415582
Sequences flagged as poor quality0
Sequence length35-75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1710643.8740985899480522TruSeq Adapter, Index 7 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT237590.538071764945142TruSeq Adapter, Index 7 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAC201330.4559534847274946TruSeq Adapter, Index 7 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAT175290.39698051128933853TruSeq Adapter, Index 7 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATT124890.2828392723767784TruSeq Adapter, Index 7 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA77510.1755374489704868TruSeq Adapter, Index 7 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC76500.17325009477799302TruSeq Adapter, Index 7 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA60080.13606360384655977TruSeq Adapter, Index 7 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA51880.11749300545205593TruSeq Adapter, Index 7 (100% over 63bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)