FastQCFastQC Report
Sun 5 Jun 2022
EGAF00006143588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006143588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6012849
Sequences flagged as poor quality0
Sequence length35-75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA3565145.929202612605106TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTTCTTGAAAAAAAAA65510.1089500168722015TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA62550.10402722569617165TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA61990.10309588682503087TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC60740.10101700541623447TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)