FastQCFastQC Report
Mon 6 Jun 2022
EGAF00006143590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006143590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2375848
Sequences flagged as poor quality0
Sequence length35-75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1355835.7067202952377425TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA30360.12778595263670067TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA27610.11621113808627488TruSeq Adapter, Index 13 (97% over 40bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26570.1118337536744775No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTTACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA24600.10354197743289975TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA24470.10299480438142508TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)