FastQCFastQC Report
Sun 5 Jun 2022
EGAF00006143596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006143596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4390759
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC1548793.527385584132493TruSeq Adapter, Index 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTTTGC933322.12564615821547TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGGTTGC700361.595077297569737TruSeq Adapter, Index 1 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGGTTGG538981.227532642989515TruSeq Adapter, Index 1 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGTATGC508291.1576358438256347TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGGATGC458271.0437147654881538TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGTTTGC385580.8781625226982396TruSeq Adapter, Index 1 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTTTGG376240.8568905740442598TruSeq Adapter, Index 1 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGGTTGC305690.6962122038581484TruSeq Adapter, Index 1 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATTC282540.6434878343357037TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTTTTC261480.5955234618889355TruSeq Adapter, Index 1 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGGTTGG225850.5143757605461835TruSeq Adapter, Index 1 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGGTTTG225200.5128953786805425TruSeq Adapter, Index 1 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGGTTTC221230.5038536617473197TruSeq Adapter, Index 1 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGGATGC163080.37141642253651364TruSeq Adapter, Index 1 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTTTTG157920.35966446803388663TruSeq Adapter, Index 1 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGTTTGG146480.33360974719860503TruSeq Adapter, Index 1 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGGGTGC145090.3304440075166959TruSeq Adapter, Index 1 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGG129630.29523369422006535TruSeq Adapter, Index 1 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGGGTGG128640.292978958763166TruSeq Adapter, Index 1 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGGATTC118010.2687690214835294TruSeq Adapter, Index 1 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGGATGG111630.2542385040946224TruSeq Adapter, Index 1 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTGTGC90630.20641078228160553TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGTATTC74690.17010726391496322TruSeq Adapter, Index 1 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGGTTTG74390.16942401074620583TruSeq Adapter, Index 1 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGTTTTC73590.16760200229618613TruSeq Adapter, Index 1 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGGTTTC71140.16202210141800086TruSeq Adapter, Index 1 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGGGTGC64780.14753713424034434TruSeq Adapter, Index 1 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATTG57770.1315717851970468TruSeq Adapter, Index 1 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGGGTTG57210.130296379282033TruSeq Adapter, Index 1 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGGGTGG52340.11920490284253815TruSeq Adapter, Index 1 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGGATTG52220.11893160157503521TruSeq Adapter, Index 1 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTGTGG51190.11658576569563486TruSeq Adapter, Index 1 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGTATGG49070.11175744330308268TruSeq Adapter, Index 1 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGGGTTC46410.1056992652067672TruSeq Adapter, Index 1 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGTTTTG45350.10328510401049111TruSeq Adapter, Index 1 (97% over 46bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)