FastQCFastQC Report
Thu 16 Jun 2022
EGAF00006143614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006143614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2516488
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGTTGG423421.6825830284110235TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGTTGC244460.9714332037347287TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGTTGG222190.8829368548548612TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTTTGC180240.7162362784960628TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGTTTG176700.7021690546507673TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTTTGG158940.6315945079014882TruSeq Adapter, Index 8 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGGTGG148490.5900683810135395TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGTTGC125100.4971213850413751TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTTTGC96450.3832722429036022TruSeq Adapter, Index 8 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC86790.34488541173254156TruSeq Adapter, Index 8 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGTTTC82500.3278378438522258TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTTTGG78740.3128963857566577TruSeq Adapter, Index 8 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTTTTG77400.3075715044140882TruSeq Adapter, Index 8 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGGTGG73890.2936234943301935TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTTTTC69010.27423138914232853TruSeq Adapter, Index 8 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGTTTG68060.27045628669796956TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGGTGC64170.2549982356363313TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGATGC61350.2437921420646552TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGGTTG61070.24267948029158098TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATGC42160.1675350726886041TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGGTGC32380.12867138647194026TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATTC31910.12680370420999426TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGATGG30990.12314781552703609TruSeq Adapter, Index 8 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTGTGG30000.11921376140080937TruSeq Adapter, Index 8 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGTTTC28990.11520023143364878TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGATGC27210.10812688159053412TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTTTTG27080.10761028862446394TruSeq Adapter, Index 8 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTTTTC26030.1034378069754356TruSeq Adapter, Index 8 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTGTGC25350.10073562838368393TruSeq Adapter, Index 8 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)