FastQCFastQC Report
Sun 5 Jun 2022
EGAF00006143618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006143618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6654200
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC5149167.738210453548135TruSeq Adapter, Index 10 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATGC3163654.75436566379129TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACTAGCTTATCTCGTATGC135380.20345045234588682TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACTAGCTTATCTCGTATGC131440.19752937994048872TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACTAGCTTATCTCGTATGC112950.16974241832226264TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACTAGCTTATCTCGTATGC112050.16838988909260316TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC111770.16796910222115358TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTTTGC104330.15678819392263532TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC82160.12347089056535723TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACTAGCTTATCGCGTATGC73050.1097802891406931TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTTTGC69930.1050915211445403TruSeq Adapter, Index 10 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACTAGCTTATCGCGTATGC68220.1025217156081873TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACTAGCTTATCGCGTATGC67090.1008235400198371TruSeq Adapter, Index 10 (97% over 42bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)