FastQCFastQC Report
Mon 6 Jun 2022
EGAF00006143630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006143630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3068064
Sequences flagged as poor quality0
Sequence length35-75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2476458.071702545970357TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA91590.29852701899308487TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAA55310.180276552249236TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA46820.15260437852665396TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA42510.13855643167808757TruSeq Adapter, Index 16 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA36630.11939125129071623TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA34010.11085166411130928TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA32960.10742931047070726TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA32620.10632111976803613TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA32610.10628852592383992TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)