FastQCFastQC Report
Mon 6 Jun 2022
EGAF00006143636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006143636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1496465
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC464593.104583134253056TruSeq Adapter, Index 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTTTGC281291.8796964847156465TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGTATGC185491.2395211381489044TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGGTTGC164871.1017297430945596TruSeq Adapter, Index 1 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGGTTGG141420.9450271138984205TruSeq Adapter, Index 1 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGGATGC133540.8923696845566051TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTTTGG132840.8876919941328397TruSeq Adapter, Index 1 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGGTTGC124050.8289535672401291TruSeq Adapter, Index 1 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGTTTGC117360.7842482116187148TruSeq Adapter, Index 1 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGGATGC108010.7217676323869919TruSeq Adapter, Index 1 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGGTTGG83790.5599195437247112TruSeq Adapter, Index 1 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATTC80390.5371993330949939TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTTTTC79710.5326552909690504TruSeq Adapter, Index 1 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTTTTG68790.4596833203583111TruSeq Adapter, Index 1 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGG57320.38303602155747046TruSeq Adapter, Index 1 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGGTTTG50080.33465533774595463TruSeq Adapter, Index 1 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGTTTGG45540.3043171741403909TruSeq Adapter, Index 1 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGGGTGC41780.27919129414987987TruSeq Adapter, Index 1 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGGATGG37380.24978866862906918TruSeq Adapter, Index 1 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGGTTTC36820.24604651629005692TruSeq Adapter, Index 1 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGGGTGG35520.23735937693163556TruSeq Adapter, Index 1 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGGGTGG27880.1863057271636824TruSeq Adapter, Index 1 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGGGTGC26710.1784873017411032TruSeq Adapter, Index 1 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATTG26290.17568068748684398TruSeq Adapter, Index 1 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGGATGG23430.15656898089831703TruSeq Adapter, Index 1 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGGATTC22890.15296047685712663TruSeq Adapter, Index 1 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGTATGG20480.13685585696959168TruSeq Adapter, Index 1 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGTGTGC20020.13378194611968874TruSeq Adapter, Index 1 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTGTGC18740.12522845505908925TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGTATTC16680.11146268038343696TruSeq Adapter, Index 1 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGTTTTC15830.10578262772600762TruSeq Adapter, Index 1 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGGTTTG15650.10457979304561082TruSeq Adapter, Index 1 (97% over 45bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)