FastQCFastQC Report
Thu 16 Jun 2022
EGAF00006143638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006143638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8794628
Sequences flagged as poor quality0
Sequence length35-75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA111946312.728940894373247TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA263490.29960334877154554TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA194390.22103265766329172TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA190030.21607508583649015TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA189570.21555203926760746TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA176610.2008157707182157TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA172580.1962334279516996TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA164780.18736437743586198TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA162470.18473777401386393TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA158480.18020091355768544TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA158470.18018954298010104TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA157530.17912070868716676TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAA141600.16100737859520606TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTTCTTGAAAAAAAAA137570.15642503582868997TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA131580.14961405985563003TruSeq Adapter, Index 18 (97% over 40bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA107610.12235878538580597TruSeq Adapter, Index 18 (97% over 40bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN103360.11752628991243291No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTTAAAAAAAAA100790.11460405147324026TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)