FastQCFastQC Report
Mon 6 Jun 2022
EGAF00006143642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006143642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3608146
Sequences flagged as poor quality0
Sequence length35-75
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA326260.90423170237568TruSeq Adapter, Index 4 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT69180.19173281790703592TruSeq Adapter, Index 4 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAT59500.16490463523371837TruSeq Adapter, Index 4 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATT51520.14278801356707851TruSeq Adapter, Index 4 (100% over 63bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)