FastQCFastQC Report
Thu 16 Jun 2022
EGAF00006143648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006143648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1932216
Sequences flagged as poor quality0
Sequence length150
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1555638.051015000393331TruSeq Adapter, Index 4 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC39140.20256534466125942TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACTGACCAATCTCGTATGC32730.16939099976400154TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACTGACCAATCTCGTATGC32230.16680329735391902TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACTGACCAATCTCGTATGC28860.14936218310996285TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACTGACCAATCTCGTATGC28590.1479648238085183TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACTGACCAATCTCGTATGC27470.14216837040993346TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACTGACCAATCTCGTATGC22860.11830975418897266TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC22290.11535977344147859TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATTGACCAATCTCGTATGC21890.11328961151341257TruSeq Adapter, Index 4 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)