FastQCFastQC Report
Mon 6 Jun 2022
EGAF00006143649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006143649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1932216
Sequences flagged as poor quality0
Sequence length150
%GC38

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1167976.044717567808154Illumina Single End PCR Primer 1 (100% over 50bp)
GAGCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG64300.3327785299366116Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGACGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG36050.18657334376694945Illumina Single End PCR Primer 1 (98% over 50bp)
AATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG20840.1078554364522393Illumina Single End PCR Primer 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)