FastQCFastQC Report
Mon 6 Jun 2022
EGAF00006143658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006143658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3370512
Sequences flagged as poor quality0
Sequence length35-75
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA3269609.700603350470196TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA93140.2763378382868834TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA56900.16881708179647484TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA54800.16258657438395116TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA54680.16223054538894982TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA53690.15929330618018864TruSeq Adapter, Index 16 (97% over 40bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53450.15858124819018593No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA49940.14816740008639637TruSeq Adapter, Index 16 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA48560.1440730666438808TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA48090.1426786197467922TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA47470.14083913660595185TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA47280.14027542403053306TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCTCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA47250.14018641678178273TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA39810.11811261909169882TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTTCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA38370.11384027115168259TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAA37020.10983494495791737TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA36580.10852950530957908TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)