FastQCFastQC Report
Sun 5 Jun 2022
EGAF00006143660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006143660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6360989
Sequences flagged as poor quality0
Sequence length35-75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA5425778.52975850139027TruSeq Adapter, Index 4 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAC2750984.324767736589389TruSeq Adapter, Index 4 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT427340.6718137698398786TruSeq Adapter, Index 4 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAT415510.6532160329156362TruSeq Adapter, Index 4 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTTAAAAAAAAAAC283900.4463142445302138TruSeq Adapter, Index 4 (98% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTTCTTGAAAAAAAAAAC225160.3539701137669001TruSeq Adapter, Index 4 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC216230.33993141632535445TruSeq Adapter, Index 4 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA175940.27659220916747385TruSeq Adapter, Index 4 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATT173560.27285065262650193TruSeq Adapter, Index 4 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAACAC162320.2551804444246013TruSeq Adapter, Index 4 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA132590.20844242931405793TruSeq Adapter, Index 4 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTTAAAAAAAAAAA106510.16744251562139156TruSeq Adapter, Index 4 (98% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTTCTTTAAAAAAAAAAC86950.13669258035189183TruSeq Adapter, Index 4 (98% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTTCTTGAAAAAAAAAAA86940.1366768595260894TruSeq Adapter, Index 4 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA84880.13343836941079446TruSeq Adapter, Index 4 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGCAAAAAAAAAC82880.1302942042503139TruSeq Adapter, Index 4 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA81270.12776315129612706TruSeq Adapter, Index 4 (98% over 63bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA78870.12399015310355041TruSeq Adapter, Index 4 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA75240.11828349333727821TruSeq Adapter, Index 4 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA74250.11672713158284034TruSeq Adapter, Index 4 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGATCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA74060.11642843589259469TruSeq Adapter, Index 4 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA74040.11639699424098987TruSeq Adapter, Index 4 (98% over 63bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA73470.11550090717025294TruSeq Adapter, Index 4 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA72230.11355152477075499TruSeq Adapter, Index 4 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA69800.10973136410077113TruSeq Adapter, Index 4 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA66670.10481074562461908TruSeq Adapter, Index 4 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAC64680.10168230128994092TruSeq Adapter, Index 4 (98% over 63bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)