FastQCFastQC Report
Sun 5 Jun 2022
EGAF00006143662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006143662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7484449
Sequences flagged as poor quality0
Sequence length35-75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA3257444.352277635935525TruSeq Adapter, Index 2 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAC1469511.96341774791972TruSeq Adapter, Index 2 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT327450.43750715650544214TruSeq Adapter, Index 2 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAT258710.34566338817994485TruSeq Adapter, Index 2 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTTAAAAAAAAAAC138900.18558480390473633TruSeq Adapter, Index 2 (98% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTTCTTGAAAAAAAAAAC126000.16834906617708265TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATT125540.1677344584751663TruSeq Adapter, Index 2 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA123910.16555660944446277TruSeq Adapter, Index 2 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA104410.13950258729800952TruSeq Adapter, Index 2 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC96700.12920122777241183TruSeq Adapter, Index 2 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAACAC85740.11455753122240528TruSeq Adapter, Index 2 (100% over 63bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)