FastQCFastQC Report
Sat 6 Aug 2022
EGAF00006143778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006143778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences648619
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC496007.647016199032097No Hit
GGGGACGCTCTTGTATTATCTGTACACTGACTTATGGTATCAAATAATAA325015.010799868643996No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA279764.313163814196008No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA262264.0433598152382215No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA253563.909228684327779No Hit
CACTTAATGAGACCAACAGTAACACCACTGACTCTGATCCAGAAGACCAA221083.408472462262129No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC220263.395830217739536No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTAGAGT194783.002995595257No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTAGAG190462.9363925509428497No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAAAGACCAAGTC185242.855913872396584No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTAGAGTGCA181142.7927026497836174No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC153602.3681082422809077No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTAGAGTG131552.0281552035940975No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTAGAG124771.923625425712167No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAG124401.917920998305631No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT108161.667542887272806No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG104931.6177447777508829No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTAGAGT97841.5084356147445572No Hit
GTGCTGGTAAATTCACTCCATCGAATCATCACCAATAGACCAAGTCTCTG97281.4998018867779082No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTAGAGTGC86831.3386903559716876No Hit
TGGGGAAAAATATGCTTTGATCTACAGAAAAACATAGAAATCAGAGACCA85441.317260209768755No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT85081.3117099560759091No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC83081.2808752133378762No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTAGAGTGCATCTATC79181.2207474649987127No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTAGAGTG78471.209801131326711No Hit
AATCTCCAAGGAAGTTGTACCGTCTTAGACCAAGTCTCTGCTACCGTAGA76541.1800456045845096No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAGA76351.1771163040243964No Hit
TCTATTGTGATGGCCACGTTAGACCAAGTCTCTGCTACCGTAGAGTGCAT73421.1319434059131785No Hit
TGGGGAAAAATATGCTTTGAGCTACAGAAAAACATAGAAATCAGAGACCA71371.1003377946066952No Hit
CAATTGAAGGTTTCCTTTTAACTCTAGTATCACACTGACTTATGGTATCA70381.085074596951369No Hit
AACAATCGACAGGCTAGAAGTTGAGACCAAGTCTCTGCTACCGTAGAGTG62540.964202405418281No Hit
TGAATTCTCCTCAGATGACTCCATTTAAAGACCAAGTCTCTGCTACCGTA62210.9591146728665055No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG55230.8515014207107716No Hit
ATTTAACACCTAGCCAAAAGGCAGACCAAGTCTCTGCTACCGTAGAGTGC52430.808332780877526No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT49140.7576096290734622No Hit
TGATAAATGCACCTGTTTGCTGTCACATTCAGAGACCAAGTCTCTGCTAC37170.5730636937863368No Hit
AATAGTGTAAAGCAGCATATAAAAATGACTCTAGGTAGACCAAGTCTCTG34490.531745138517373No Hit
GACTACAGATGTGCCACCATACCCAGAGACCAAGTCTCTGCTACCGTAGA33060.5096982974596798No Hit
TTCAGGTGTATTTTGGAGGCCAGGTGTGGAGACCAAGTCTCTGCTACCGT31500.48564719812401425No Hit
CACAAAGGTTTTTATAAGTTCTGTGGATCTAGACCAAGTCTCTGCTACCG31460.4850305032692536No Hit
AAGTCCTCCATTTCTGTATCCATGCGAGACCAAGTCTCTGCTACCGTAGA30420.4689964370454766No Hit
TCAGATATAAAAGAAGAGGTCTTGGCCAGTTGGAGACCAAGTCTCTGCTA29280.451420633684798No Hit
AGCAATCTGCTTGTGTGTGCGTGAGACCAAGTCTCTGCTACCGTAGAGTG28450.43862421544851443No Hit
GGGAAAAGAACAGGCTTCACCTAAAAACGTAGACCAAGTCTCTGCTACCG25570.3942221859057475No Hit
AACTGAGCGACCTCAGAGACCAAGTCTCTGCTACCGTAGAGTGCATCTAT25400.3916012327730147No Hit
ATCTCCTGGTTATTCTCAGTTTTCTTTACCAGTGCAAGACCAAGTCTCTG24460.3771089036861393No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCAGAGTATTGCCAATCATGTTC23740.36600839630044757No Hit
ATGGTTGATAGAGTGGTAGACCTTGCAGACCAAGTCTCTGCTACCGTAGA23690.36523752773199675No Hit
GCTATTCCTACCATTCTGATGAGGTAGACCAAGTCTCTGCTACCGTAGAG23550.3630790957403345No Hit
CACCATTTATCTGAGGACTGGAGACCAAGTCTCTGCTACCGTAGAGTGCA23170.35722049462010824No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC22640.3490492877945296No Hit
TCCAAAGTAATATCCAATGTAAAAGATGCAGACCAAGTCTCTGCTACCGT18210.28075033262978727No Hit
TCAAAGGGCCCCAGACTGCAGACCAAGTCTCTGCTACCGTAGAGTGCATC18100.2790544217791955No Hit
TTCAGGAAGAAGAACGATTATCCAGAAAGACCAAGTCTCTGCTACCGTAG15930.24559872590843007No Hit
AAGGGAAAAGGATGGATTAGGGTACACTGACGACATGGTTCTACTACCCT15060.23218561281738584No Hit
GAGTTTGATGCACAACTTCAAGGCAATCTCAAGACCAAGTCTCTGCTACC14480.2232435374233564No Hit
TTCTGATGAGCCAAGTCTCTGTGAGACCAAGTCTCTGCTACCGTAGAGTG12570.1937963581085352No Hit
CATTGGTGCTAAAAGTTTCTTGGATCACATTTTAGACCAAGTCTCTGCTA12220.18840027812937948No Hit
TTAACTACAATTCCCAGAAGAGCCCAATTCGAGACCAAGTCTCTGCTACC12160.18747523584723852No Hit
CGGGAGCCCACTTCATTAGTACTGGAGACCAAGTCTCTGCTACCGTAGAG11470.17683724960261724No Hit
AAGAAGAACAAACAACAAGACCAAGTCTCTGCTACCGTAGAGTGCATCTA10810.16666178449906646No Hit
TGCATTCTGAAAAAGCCAGAATTTACAGCTCTCCAGACCAAGTCTCTGCT9920.15294032398064195No Hit
GTAGACAACATAAAGCCTCATATGTTAATTGCTGCAGACCAAGTCTCTGC9760.15047354456159934No Hit
ACACCACTCTCTAAATCAAACACATTGGAGACCAAGTCTCTGCTACCGTA9650.14877763371100755No Hit
AGTAATCGCATGGTGGTGGTAATTTTAAAGCCCTAGACCAAGTCTCTGCT9090.1401439057443584No Hit
AAAGTGAGGCACTCCAGTTTTATTTAAGTATTCCCAATATAAGTAGACCA8890.13706043147055513No Hit
CTACTCTTAATCTTCCTCTCCAGAATAATCACAAGACCAAGTCTCTGCTA8770.13521034690627318No Hit
ATCCTGTTCCAAGTCAAGACCAAGTCTCTGCTACCGTAGAGTGCATCTAT8490.13089348292294858No Hit
GGATGAGAACTCCGAGAAGCACCTTCTCCCCTTGATCATTAAGACCAAGT8410.1296600932134273No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA8330.128426703503906No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAGAGACCAAGTCTCTGCTACC8310.12811835607652566No Hit
TCTGATCAAAGAAGAGGAGTATGATTCTATAGACCAAGTCTCTGCTACCG7860.12118053896046832No Hit
TCAATCATTCTCTACAGCCCAGACCAAGTCTCTGCTACCGTAGAGTGCAT7790.12010132296463717No Hit
GCTCTTCCCCCCAAAAAATAAATCAATAAAAGTTTTCAGACCAAGTCTCT7600.11717202240452407No Hit
GGGAAGCTCTTCATCCTCACGAGACCAAGTCTCTGCTACCGTAGAGTGCA7300.11254681099381918No Hit
GACTACAGATGTGCCACCAGACCAAGTCTCTGCTACCGTAGAGTGCATCT6810.10499229902300117No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)