FastQCFastQC Report
Sat 6 Aug 2022
EGAF00006143779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006143779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences648619
Sequences flagged as poor quality0
Sequence length150
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG488787.535702777747799No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTACAGATAATACAAG316354.877285432588314No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT274234.227905750525347No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT258013.9778359869199025No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT249083.8401588605945864No Hit
TCTGGATCAGAGTCAGTGGTGTTACTGTTGGTCTCATTAAGTGTGTAGAA217703.356361747034854No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG215783.326760394006343No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT191972.959672781710064No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA188382.9043244184952957No Hit
TTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA182182.8087367160073944No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC178152.7466047093902586No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG151592.337119325829185No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC130132.006262536250094No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA123141.8984951103806702No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA122981.896028330961628No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT106711.6451876987877323No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT102851.5856766453033289No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT96201.4831511256993704No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACTGTAGAACCATGTC94871.4626460217785788No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT86371.33159836514194No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG84191.2979884955574843No Hit
CTGATTTCTATGTTTTTCTGTAGATCAAAGCATATTTTTCCCCATGTAGA83511.2875046830265533No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG81581.2577491562843517No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT80781.2454152591891388No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC77041.1877542902690177No Hit
AAGACGGTACAACTTCCTTGGAGATTTGTAGAACCATGTCGTCAGTGTAG75181.1590779795226474No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG75021.1566112001036049No Hit
AACGTGGCCATCACAATAGATGTAGAACCATGTCGTCAGTGTAGATCTCG72231.1135967339840493No Hit
CTGATTTCTATGTTTTTCTGTAGCTCAAAGCATATTTTTCCCCATGTAGA69741.0752074792751987No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTGATACTAGAGTTAA69011.0639527981758166No Hit
CAACTTCTAGCCTGTCGATTGTTTGTAGAACCATGTCGTCAGTGTAGATC61700.9512518134683073No Hit
TTAAATGGAGTCATCTGAGGAGAATTCATGTAGAACCATGTCGTCAGTGT61080.9416930432195172No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT53250.8209750254001194No Hit
GCCTTTTGGCTAGGTGTTAAATTGTAGAACCATGTCGTCAGTGTAGATCT51300.7909111512305376No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT48650.7500551171026443No Hit
CTGAATGTGACAGCAAACAGGTGCATTTATCATGTAGAACCATGTCGTCA36970.5699802195125335No Hit
ACCTAGAGTCATTTTTATATGCTGCTTTACACTATTTGTAGAACCATGTC33900.5226488894096535No Hit
CTGGGTATGGTGGCACATCTGTAGTCTGTAGAACCATGTCGTCAGTGTAG32620.5029146540573125No Hit
CCACACCTGGCCTCCAAAATACACCTGAATGTAGAACCATGTCGTCAGTG31420.48441380841449294No Hit
AGATCCACAGAACTTATAAAAACCTTTGTGTGTAGAACCATGTCGTCAGT31120.47978859700378806No Hit
CGCATGGATACAGAAATGGAGGACTTTGTAGAACCATGTCGTCAGTGTAG29820.4597460142240668No Hit
CCAACTGGCCAAGACCTCTTCTTTTATATCTGATGTAGAACCATGTCGTC28970.44664124856040294No Hit
CACGCACACACAAGCAGATTGCTTGTAGAACCATGTCGTCAGTGTAGATC28230.4352323937473309No Hit
CTGAGGTCGCTCAGTTTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG25640.3953014019015786No Hit
ACGTTTTTAGGTGAAGCCTGTTCTTTTCCCTGTAGAACCATGTCGTCAGT25070.38651350022123926No Hit
TGCACTGGTAAAGAAAACTGAGAATAACCAGGAGATTGTAGAACCATGTC24210.37325456084388525No Hit
GCAAGGTCTACCACTCTATCAACCATTGTAGAACCATGTCGTCAGTGTAG23860.36785848086472955No Hit
ACCTCATCAGAATGGTAGGAATAGCTGTAGAACCATGTCGTCAGTGTAGA23420.36107483746236235No Hit
CCAGTCCTCAGATAAATGGTGTGTAGAACCATGTCGTCAGTGTAGATCTC22840.3521327620683329No Hit
TTGTGAAGAGAACATGATTGGCAATACTCTGAAGTATCTACACAATAAAA22530.3473533769439378No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA22260.3431906866743034No Hit
GCATCTTTTACATTGGATATTACTTTGGATGTAGAACCATGTCGTCAGTG18090.27890024806550534No Hit
GCAGTCTGGGGCCCTTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGG17590.2711915623809972No Hit
TTCTGGATAATCGTTCTTCTTCCTGAATGTAGAACCATGTCGTCAGTGTA15570.24004847221558417No Hit
CCAAAGCAGAAAGGGTAGTAGAACCATGTCGTCAGTGTACCCTAATCCAT15050.23203143910369572No Hit
TGAGATTGCCTTGAAGTTGTGCATCAAACTCTGTAGAACCATGTCGTCAG14310.22062258429062362No Hit
CACAGAGACTTGGCTCATCAGAATGTAGAACCATGTCGTCAGTGTAGATC12380.19086705754842212No Hit
AAAATGTGATCCAAGAAACTTTTAGCACCAATGTGTAGAACCATGTCGTC12130.187012714706168No Hit
CGAATTGGGCTCTTCTGGGAATTGTAGTTAATGTAGAACCATGTCGTCAG12010.18516263014188605No Hit
CCAGTACTAATGAAGTGGGCTCCCGTGTAGAACCATGTCGTCAGTGTAGA11340.17483299132464514No Hit
TGTTGTTTGTTCTTCTTTGTAGAACCATGTCGTCAGTGTAGATCTCGGTG10710.16512004736216485No Hit
GGAGAGCTGTAAATTCTGGCTTTTTCAGAATGCATGTAGAACCATGTCGT9640.14862345999731738No Hit
GCAGCAATTAACATATGAGGCTTTATGTTGTCTACTGTAGAACCATGTCG9580.1476984177151764No Hit
CCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT9560.14739007028779608No Hit
AGGGCTTTAAAATTACCACCACCATGCGATTACTTGTAGAACCATGTCGT9030.13921886346221743No Hit
ACTTATATTGGGAATACTTAAATAAAACTGGAGTGCCTCACTTTTGTAGA8720.13443947833782235No Hit
TGTGATTATTCTGGAGAGGAAGATTAAGAGTAGTGTAGAACCATGTCGTC8670.13366860976937153No Hit
TGACTTGGAACAGGATTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG8580.13228104634616006No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT8420.12981426692711748No Hit
TAATGATCAAGGGGAGAAGGTGCTTCTCGGAGTTCTCATCCTGTAGAACC8260.12734748750807484No Hit
CTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG8100.12488070808903225No Hit
GGGCTGTAGAGAATGATTGATGTAGAACCATGTCGTCAGTGTAGATCTCG7780.11994714925094702No Hit
ATAGAATCATACTCCTCTTCTTTGATCAGATGTAGAACCATGTCGTCAGT7670.11825123840035522No Hit
GAAAACTTTTATTGATTTATTTTTTGGGGGGAAGAGCTGTAGAACCATGT7400.11408854813072082No Hit
CGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC7140.11008003157477657No Hit
GGTGGCACATCTGTAGTCTGTAGAACCATGTCGTCAGTGTAGATCTCGGT6730.10375890931347986No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)