FastQCFastQC Report
Sat 6 Aug 2022
EGAF00006143861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006143861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences669402
Sequences flagged as poor quality0
Sequence length150
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG464946.945602194197209No Hit
TTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA302254.5152240357811895No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT256883.837454922453175No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT243943.644148060507737No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTACAGATAATACAAG230113.4375457497886175No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG203873.04555409156232No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG187222.7968246285490634No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT180992.703756487133292No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA175762.6256270522048037No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC150582.2494704228550257No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT144482.1583443132826017No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA142462.1281681261782905No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA134322.0065670553718093No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC128981.92679436272972No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT116591.7417037893522878No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG110101.644751584249823No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACTGTAGAACCATGTC107181.6011305613069575No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG96421.440390079503796No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT94421.4105126665292307No Hit
AAGACGGTACAACTTCCTTGGAGATTTGTAGAACCATGTCGTCAGTGTAG90851.3571814843696315No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT87861.3125147519726563No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG86371.2902560793066051No Hit
TTAAATGGAGTCATCTGAGGAGAATTCATGTAGAACCATGTCGTCAGTGT81491.2173551916486656No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT80091.1964410025664698No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT78541.1732860075111815No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC75801.132353951736027No Hit
AACGTGGCCATCACAATAGATGTAGAACCATGTCGTCAGTGTAGATCTCG70981.0603493864673246No Hit
CAACTTCTAGCCTGTCGATTGTTTGTAGAACCATGTCGTCAGTGTAGATC67701.0113504291890374No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT66560.9943203037935351No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT63070.9421842181529185No Hit
GCCTTTTGGCTAGGTGTTAAATTGTAGAACCATGTCGTCAGTGTAGATCT62780.9378519932716066No Hit
CTGATTTCTATGTTTTTCTGTAGATCAAAGCATATTTTTCCCCATGTAGA60350.9015509365075096No Hit
CTGATTTCTATGTTTTTCTGTAGCTCAAAGCATATTTTTCCCCATGTAGA57310.8561372687861702No Hit
ACCTAGAGTCATTTTTATATGCTGCTTTACACTATTTGTAGAACCATGTC53890.8050468925996636No Hit
CCAACTGGCCAAGACCTCTTCTTTTATATCTGATGTAGAACCATGTCGTC52980.7914526696962363No Hit
GCAGTCTGGGGCCCTTTGCTCTGCCACACACAGTCGGGAAACAAGCATAG52430.7832363811282309No Hit
CTGAATGTGACAGCAAACAGGTGCATTTATCATGTAGAACCATGTCGTCA45920.6859854018960206No Hit
AGATCCACAGAACTTATAAAAACCTTTGTGTGTAGAACCATGTCGTCAGT41860.6253342535576529No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA40340.6026274196969833No Hit
ACGTTTTTAGGTGAAGCCTGTTCTTTTCCCTGTAGAACCATGTCGTCAGT39590.5914233898315213No Hit
CGCATGGATACAGAAATGGAGGACTTTGTAGAACCATGTCGTCAGTGTAG39510.5902282933125387No Hit
CTGGGTATGGTGGCACATCTGTAGTCTGTAGAACCATGTCGTCAGTGTAG38460.5745426515008918No Hit
TGAGATTGCCTTGAAGTTGTGCATCAAACTCTGTAGAACCATGTCGTCAG38270.5717042972683081No Hit
TGCACTGGTAAAGAAAACTGAGAATAACCAGGAGATTGTAGAACCATGTC37370.5582594614297537No Hit
CACGCACACACAAGCAGATTGCTTGTAGAACCATGTCGTCAGTGTAGATC36770.5492962375373841No Hit
CAGAAATGACATCTACCTGTTACTCACAGCACCGACTACATACAGTGTAG35760.5342081439852286No Hit
GCAAGGTCTACCACTCTATCAACCATTGTAGAACCATGTCGTCAGTGTAG30950.46235296578139895No Hit
ACCTCATCAGAATGGTAGGAATAGCTGTAGAACCATGTCGTCAGTGTAGA29450.4399449060504749No Hit
CTGAGGTCGCTCAGTTTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG27430.4097687189461639No Hit
CCACACCTGGCCTCCAAAATACACCTGAATGTAGAACCATGTCGTCAGTG27370.4088723965569269No Hit
CCAGTCCTCAGATAAATGGTGTGTAGAACCATGTCGTCAGTGTAGATCTC27070.4043907846107421No Hit
TTGTGAAGAGAACATGATTGGCAATACTCTGAAGTATCTACACAATAAAA25760.3848210791124018No Hit
CGAATTGGGCTCTTCTGGGAATTGTAGTTAATGTAGAACCATGTCGTCAG25300.3779492741282518No Hit
TCATTTACAGTTTGTGGGTATGCATGTGTGGAAGCTCTAAGGGATGTAGA23860.3564375367865647No Hit
AAAATGTGATCCAAGAAACTTTTAGCACCAATGTGTAGAACCATGTCGTC23840.35613876265681904No Hit
GCATCTTTTACATTGGATATTACTTTGGATGTAGAACCATGTCGTCAGTG23740.3546448920080908No Hit
ACTTATATTGGGAATACTTAAATAAAACTGGAGTGCCTCACTTTTGTAGA22320.3334319287961494No Hit
TGCTATCTGATGTATGTTCCAATATTTCACCAAACATATTTCGAAGTTCA21900.32715767207149066No Hit
GCAGCAATTAACATATGAGGCTTTATGTTGTCTACTGTAGAACCATGTCG20580.3074385795082775No Hit
TTCTGGATAATCGTTCTTCTTCCTGAATGTAGAACCATGTCGTCAGTGTA19640.2933961954102318No Hit
CAAATACCAGTGAACTTAAAGAATTCGACCCTTAGAAACTACATGTAGAA18760.28025013370142304No Hit
CCAGTACTAATGAAGTGGGCTCCCGTGTAGAACCATGTCGTCAGTGTAGA17720.2647138789546491No Hit
GCAGTCTGGGGCCCTTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGG17580.2626224600464295No Hit
ACAAGTCAACAACCCCCACAGTGAGCACACCCCAACTTGCTCATTGTAGA17370.2594853316841001No Hit
AGGGCTTTAAAATTACCACCACCATGCGATTACTTGTAGAACCATGTCGT15250.22781527393106088No Hit
CACAGAGACTTGGCTCATCAGAATGTAGAACCATGTCGTCAGTGTAGATC14150.2113826967950499No Hit
TGTGATTATTCTGGAGAGGAAGATTAAGAGTAGTGTAGAACCATGTCGTC13120.19599582911314872No Hit
GAAAACTTTTATTGATTTATTTTTTGGGGGGAAGAGCTGTAGAACCATGT12610.18837708880463458No Hit
GGGCTGTAGAGAATGATTGATGTAGAACCATGTCGTCAGTGTAGATCTCG12520.18703260522077916No Hit
CGTCTTCCCGCGCATCGTGTAGAACCATGTCGTCAGTGTAGATCTCGGTG10960.1637282231006182No Hit
ATGATCCATGGACATTTGACTGGTAAGAAGCACATATTTGTAGAACCATG10840.16193557832214425No Hit
TGACTTGGAACAGGATTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG10750.1605910947382888No Hit
TGTTGTTTGTTCTTCTTTGTAGAACCATGTCGTCAGTGTAGATCTCGGTG9800.14639932357537025No Hit
ATAGAATCATACTCCTCTTCATCAACACAAAAACTTGTAGAACCATGTCG9750.14565238825100613No Hit
CTGGGTATGGTGGCACATCTGTAATCCCAGCACTTTGGGAAACACAACAC9200.13743609968300063No Hit
ATAGAATCATACTCCTCTTCTTTGATCAGATGTAGAACCATGTCGTCAGT9070.1354940678396539No Hit
GGAGAGCTGTAAATTCTGGCTTTTTCAGAATGCATGTAGAACCATGTCGT8180.12219861906597232No Hit
GAAAACTTTTATTGATTTATTTTTGCAGGGTGAAGAGCTGTAGAACCATG7720.11532681408182228No Hit
CCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT6990.10442155834610592No Hit
GAACGAAGCTCTATCTGCCCAATTAGCATGTAGAACCATGTCGTCAGTGT6760.1009856558540309No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)