FastQCFastQC Report
Sat 6 Aug 2022
EGAF00006143864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006143864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1190076
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC777196.530591323579335No Hit
GGGGACGCTCTTGTATTATCTGTACACTGACTTATGGTATCAAATAATAA423873.561705302854608No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA420733.5353204333168637No Hit
TCTAAGCATAGCATTCAATTTTGGCCACTACAAATCATTCTCCTTAAGAC414523.4831388919699244No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA409783.443309502922502No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC362883.049217024795055No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC325932.73873265236842No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTACTAG300672.526477300609373No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTACTA300022.521015464558566No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAAAGACCAAGTC288232.4219461614216233No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC257182.1610384546869277No Hit
TGGGGAAAAATATGCTTTGATCTACAGAAAAACATAGAAATCAGAGACCA247402.078858829184018No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTACTAGAGT242062.033987745320467No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAC228201.9175245950678779No Hit
TGGGGAAAAATATGCTTTGAGCTACAGAAAAACATAGAAATCAGAGACCA227931.9152558324006197No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTACTA226811.905844668743845No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTACTAGA213061.7903058292075464No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC199541.6766996393507643No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT199501.676363526363022No Hit
GTGCTGGTAAATTCACTCCATCGAATCATCACCAATAGACCAAGTCTCTG192171.6147708213593082No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT165291.3889028935967114No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTACTAGAG158801.3343685613355785No Hit
AATCTCCAAGGAAGTTGTACCGTCTTAGACCAAGTCTCTGCTACCGTACT154621.2992447541165437No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTACT149801.2587431390936377No Hit
TGAATTCTCCTCAGATGACTCCATTTAAAGACCAAGTCTCTGCTACCGTA144731.2161408178973443No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTACTAG138691.1653877567483084No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTACTAGA119521.0043056073729744No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTACTAGAGTATCATC118330.9943062459876513No Hit
TCTATTGTGATGGCCACGTTAGACCAAGTCTCTGCTACCGTACTAGAGTA114200.9596025800032939No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT112070.9417045634060347No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG111760.9390996877510345No Hit
AACAATCGACAGGCTAGAAGTTGAGACCAAGTCTCTGCTACCGTACTAGA109520.9202773604374846No Hit
GGAAACCGTAGCTGCCCTGGGTTCAATTTAGCTCCTGCTCACACCACCTC105310.8849014684776434No Hit
ATTTAACACCTAGCCAAAAGGCAGACCAAGTCTCTGCTACCGTACTAGAG103960.8735576551413523No Hit
AATAGTGTAAAGCAGCATATAAAAATGACTCTAGGTAGACCAAGTCTCTG96070.8072593683092509No Hit
TGATAAATGCACCTGTTTGCTGTCACATTCAGAGACCAAGTCTCTGCTAC93260.7836474309203781No Hit
TCAGATATAAAAGAAGAGGTCTTGGCCAGTTGGAGACCAAGTCTCTGCTA90660.7618000867171508No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG88340.7423055334281172No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTACTAGAG83970.7055851895173082No Hit
ATCTCCTGGTTATTCTCAGTTTTCTTTACCAGTGCAAGACCAAGTCTCTG69930.5876095308198803No Hit
GACTACAGATGTGCCACCATACCCAGAGACCAAGTCTCTGCTACCGTACT69810.5866011918566546No Hit
CACAAAGGTTTTTATAAGTTCTGTGGATCTAGACCAAGTCTCTGCTACCG69010.5798789321018153No Hit
GGGAAAAGAACAGGCTTCACCTAAAAACGTAGACCAAGTCTCTGCTACCG68960.5794587908671379No Hit
CTGTATGTAGTCGGTGCTGTGAGTAACAGGTAGATGTCATTTCTGAGACC68620.5766018304713312No Hit
AAGTCCTCCATTTCTGTATCCATGCGAGACCAAGTCTCTGCTACCGTACT64960.545847492092942No Hit
AGCAATCTGCTTGTGTGTGCGTGAGACCAAGTCTCTGCTACCGTACTAGA64630.5430745599440708No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG61390.5158494079369721No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCAGAGTATTGCCAATCATGTTC60040.504505594600681No Hit
TTCAGGTGTATTTTGGAGGCCAGGTGTGGAGACCAAGTCTCTGCTACCGT55250.4642560643185813No Hit
GAGTTTGATGCACAACTTCAAGGCAATCTCAAGACCAAGTCTCTGCTACC50620.4253509859874495No Hit
ATGGTTGATAGAGTGGTAGACCTTGCAGACCAAGTCTCTGCTACCGTACT49420.4152675963551907No Hit
AACTGAGCGACCTCAGAGACCAAGTCTCTGCTACCGTACTAGAGTATCAT47640.4003105684006736No Hit
GCTATTCCTACCATTCTGATGAGGTAGACCAAGTCTCTGCTACCGTACTA47530.39938625768438324No Hit
TTAACTACAATTCCCAGAAGAGCCCAATTCGAGACCAAGTCTCTGCTACC46770.393000110917286No Hit
AAAGTGAGGCACTCCAGTTTTATTTAAGTATTCCCAATATAAGTAGACCA45620.3833368625197046No Hit
CACCATTTATCTGAGGACTGGAGACCAAGTCTCTGCTACCGTACTAGAGT43970.3694722017753488No Hit
TCCAAAGTAATATCCAATGTAAAAGATGCAGACCAAGTCTCTGCTACCGT41160.34586026438647616No Hit
TTCAGGAAGAAGAACGATTATCCAGAAAGACCAAGTCTCTGCTACCGTAC39590.33266782961760427No Hit
TGTAGTTTCTAAGGGTCGAATTCTTTAAGTTCACTGGTATTTGAGACCAA39510.33199560364212033No Hit
GTAGACAACATAAAGCCTCATATGTTAATTGCTGCAGACCAAGTCTCTGC38960.3273740500606684No Hit
GCTGCCCCAAAGTGTATAGAAGCCAAAATGATAAGAAACTAAGAGACCAA38740.3255254286280876No Hit
CATTGGTGCTAAAAGTTTCTTGGATCACATTTTAGACCAAGTCTCTGCTA38630.32460111791179724No Hit
TCAAAGGGCCCCAGACTGCAGACCAAGTCTCTGCTACCGTACTAGAGTAT36530.3069551860553444No Hit
GCTGCCCCAAAGTGTAAAGAAGCCAAAATGATAAGAAACTAAGAGACCAA36340.30535864936357004No Hit
CGGGAGCCCACTTCATTAGTACTGGAGACCAAGTCTCTGCTACCGTACTA32370.2719994353301806No Hit
TGCATTCTGAAAAAGCCAGAATTTACAGCTCTCCAGACCAAGTCTCTGCT29620.24889166742292088No Hit
AGTAATCGCATGGTGGTGGTAATTTTAAAGCCCTAGACCAAGTCTCTGCT27480.23090962257872605No Hit
GTCTCGGAGGCTGGCATCCCTGTATGTGCTAGTGCCTGACCTGGGTGCAC26360.2214984589219512No Hit
CTACTCTTAATCTTCCTCTCCAGAATAATCACAAGACCAAGTCTCTGCTA24840.20872616538775676No Hit
GCTCTTCCCCCCAAAAAATAAATCAATAAAAGTTTTCAGACCAAGTCTCT24420.2051969790164662No Hit
AATATGTGCTTCTTACCAGTCAAATGTCCATGGATCATAGACCAAGTCTC22820.19175245950678782No Hit
TTCTGATGAGCCAAGTCTCTGTGAGACCAAGTCTCTGCTACCGTACTAGA21620.18166906987452902No Hit
AACTGAGCGACCTCAGAGCCGTTTGTTGTGCTGACCAACATGGTTTGGCT21330.17923225071339982No Hit
AGTTTTTGTGTTGATGAAGAGGAGTATGATTCTATAGACCAAGTCTCTGC20750.1743586123911414No Hit
ATCCTGTTCCAAGTCAAGACCAAGTCTCTGCTACCGTACTAGAGTATCAT18680.15696476527549502No Hit
CGATGCGCGGGAAGACGAGACCAAGTCTCTGCTACCGTACTAGAGTATCA16360.13747021198646137No Hit
TCTGATCAAAGAAGAGGAGTATGATTCTATAGACCAAGTCTCTGCTACCG16090.13520144931920314No Hit
GCTCTTCACCCTGCAAAAATAAATCAATAAAAGTTTTCAGACCAAGTCTC16020.1346132515906547No Hit
AAGAAGAACAAACAACAAGACCAAGTCTCTGCTACCGTACTAGAGTATCA15570.13083198047855768No Hit
TTTTTGTCCAGCCAGCAAATGATCAGCCTTGCAAAGACCAAGTCTCTGCT15020.1262104268971057No Hit
GCTATTCCTGTCATTCTGTTGAAGGTAAGATAGACCAAGTCTCTGCTACC13280.11158951193033051No Hit
TACTGAATGCAAATGATCCCAAGTGGTCCAGACCAAGTCTCTGCTACCGT12810.10764018432436247No Hit
CTACTCTTTAGCTTCCTACCTAAGAATAATCACAAGACCAAGTCTCTGCT12780.107388099583556No Hit
TGCTAATTGGGCAGATAGAGCTTCGTTCAGACCAAGTCTCTGCTACCGTA12630.10612767587952365No Hit
GGAAACACTGATGGGCCTGAATCAAGGCAATGTCCCTGGGGTTTGGAAAG12210.1025984895082331No Hit
GACTACAGATGTGCCACCAGACCAAGTCTCTGCTACCGTACTAGAGTATC11980.10066583982871681No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)