FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006143985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006143985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences956862
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGTTATCATCTCGTAT232482.4296084492852676TruSeq Adapter, Index 14 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGTTATCATCGCGTAT141781.481718366911843TruSeq Adapter, Index 14 (97% over 37bp)
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA46740.48847169184271083No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT30000.3135248343021251No Hit
GTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCT26720.2792461190850927No Hit
CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT22930.23963748168492424No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC20340.21256983765684082No Hit
CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT19710.20598581613649616No Hit
CGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAA17800.1860247350192609No Hit
GCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAG17760.18560670190685805No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT17710.18508416051635448No Hit
GGCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAA17680.18477063568205238No Hit
GTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAAT16470.17212513403186666No Hit
GGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAG14320.14965585424021435No Hit
GGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGG14210.14850626318110657No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA14160.14798372179060304No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA13640.1425492913293662No Hit
TATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATC13410.1401456009330499No Hit
CCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAGG13310.13910051815204283No Hit
CGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCA12750.13324805457840316No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA12500.13063534762588544No Hit
GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA12480.13042633106968404No Hit
AGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAG12430.12990378967918048No Hit
GCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAG11820.12352878471503727No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11660.12185665226542594No Hit
CCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATA10920.11412303968597352No Hit
CCAGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGC10580.11056975823054944No Hit
GTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCAC10430.10900213405903882No Hit
CCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTC10340.10806155955613245No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA10290.1075390181656289No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT10240.10701647677512537No Hit
GCCCGTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGA10140.10597139399411827No Hit
CCCCGCTTCGCGCCCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCCTT10010.10461278637880908No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA9980.10429926154450694No Hit
GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG9850.10294065392919773No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)