FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006144041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006144041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53327606
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGATCCACGGCCGCTGAATCTCGATGGGACCCCACTTTGTAAAGTAG2897550.5433489738879335No Hit
GGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTC2871840.5385278311574684No Hit
GTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAA2609360.4893075455140439No Hit
GTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGT1979370.37117173420460686No Hit
GTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTG1787420.33517724384627356No Hit
GTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT1603250.30064166015628No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC1585080.2972344192611984No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC1507140.28261909975857535No Hit
CCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACT1430410.26823067962210795No Hit
GGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCA1320670.2476522197527487No Hit
CTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGC1294750.24279169779344678No Hit
GGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTC1290630.2420191148276936No Hit
GGCAGTAATAAATTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGA1266200.23743799787299658No Hit
GTGAGAGTGAATTCTGTCCCAGATCCACGGCCGCTGAATCTCGATGGGAC1231830.23099293075335126No Hit
GCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCA1205550.22606490154461462No Hit
GCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAA1190080.22316396502029362No Hit
GGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCT1130930.21207214889788975No Hit
GGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTT1116880.20943749096856137No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC1088640.20414192229067998No Hit
GTGAATTCTGTCCCAGATCCACGGCCGCTGAATCTCGATGGGACCCCACT1023500.19192686054573685No Hit
GGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGT999620.1874488796665652No Hit
CCTGGTTTCTGCTGATACCAGGCCAACCAGCTAGTAATACTCTGACTGGC933660.1750800514090207No Hit
CCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT901400.1690306517791179No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA900810.16892001489809985No Hit
GTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCGAGAATAAGTATTA884050.1657771773966377No Hit
GTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAG881660.16532900426844588No Hit
GTTGGCAGTAATAAATTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTG873710.16383821917676183No Hit
CTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGA865860.16236618609880968No Hit
CCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGG851860.15974090417634723No Hit
CCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGG830770.15578610448029487No Hit
GGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTAGTAATACT815720.15296392641364773No Hit
GCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCC800650.15013799794425425No Hit
GCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACC789320.14801339478843284No Hit
AGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAG780940.14644197603770173No Hit
GCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCGAGA777070.14571627310627822No Hit
CCTTGGCCGAACGTCCGAGAATAAGTATTATACTGTTGGCAGTAATAAAT773370.14502244859819882No Hit
CCGGGATGGGGGCAGGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGG764900.14343415303510906No Hit
TGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCT763670.143203503266207No Hit
GGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCC756840.14192274072831998No Hit
GGCTCTTCTGTGTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCAT748970.1404469572476214No Hit
CTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTTCTCGATGG735260.13787605616498141No Hit
CTTATAGATTAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGG734500.13773354086061917No Hit
GCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGG732910.13743538384228235No Hit
CTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCGCTGGGATAGA731270.13712785081707962No Hit
GTCGGAGTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCC714390.1339625108991392No Hit
GCCCGATCTCTAGCACAATAATATACGGCTGTGTCGTCGGCTTTCAGGCT711040.13333431843912139No Hit
GTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTC707900.1327455052079405No Hit
CCGAGAATAAGTATTATACTGTTGGCAGTAATAAATTGCAAAATCATCAG698490.1309809407157711No Hit
CCCACTTTGTAAAGTAGACGCCTTATAGATTAGGAGTTTAGGGGCTTTCC695590.13043713231754675No Hit
GTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAG688500.12910761454395683No Hit
GTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACATTCC669000.12545097186624127No Hit
GGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG661040.12395831157318406No Hit
GGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTG653880.12261566738998185No Hit
CTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTT652070.12227625594143492No Hit
GTCGTAGTAGCTCCCACCATATAAATTCGCCCGATCTCTAGCACAATAAT649200.12173807314733011No Hit
GCCTTATAGATTAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCA648720.12164806348141712No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG648070.12152617539215993No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGA646690.12126739760266005No Hit
GGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACGGCCGCTGAAT634070.11890089346969748No Hit
CTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTC627950.11775327022930675No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT626800.1175376220713902No Hit
GGCCGCTGAATCTCGATGGGACCCCACTTTGTAAAGTAGACGCCTTATAG626780.11753387166864382No Hit
GGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTG612980.11494609377364512No Hit
CCCGGGATGGGGGCAGGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTG605580.11355844475748639No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT603450.11315902686499747No Hit
CTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCT600900.11268085051483465No Hit
CCTCCTGCCACCTGCTCTTGTCCACGGTTAGCCTGCTGTAGAGGAAGAAG594160.11141696478930631No Hit
CTCTTCTGTGTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGA591750.11096504125836815No Hit
CTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTG585640.1098192932193506No Hit
CCCTGGTTTCTGCTGATACCAGGCCAACCAGCTAGTAATACTCTGACTGG580970.10894357417807206No Hit
GCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCT574530.10773594449373933No Hit
CAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGG568970.10669333253024708No Hit
GTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCGAGAATAAGT567130.10634829547758061No Hit
GGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGG559990.10500940169712475No Hit
CTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTTCTCGATGGGGGCTGGG545390.10227160769227105No Hit
CGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG543920.10199595309041248No Hit
CGGCTGTGTCGTCGGCTTTCAGGCTATTCATTTGCAGATAGACGATGCTG542270.10168654486383656No Hit
GCCTGCTGTAGAGGAAGAAGGAGCCGTCGGAGTCCAGCACGGGAGGCGTG540800.101410890261978No Hit
GTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATG539000.10107335401480426No Hit
CCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGC538440.10096834273790577No Hit
GGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCC535610.10043766074929372No Hit
GGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACG535540.1004245343396814No Hit
GTTGGAGTTATCTCTTGAGATGGTGAATCTGTCTTTCACAGACGCGGCGT534960.10031577266003654No Hit
GCGTAAAGTGTTGTCTCAGCATAAGTTTTGCTTCTAATATAGCCTACCCA533950.1001263773213446No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)