FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006144042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006144042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53327606
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC6794681.2741393266369392No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT5685371.0661213631078807No Hit
GGAGCCCCAGCCGTGAGATTCCCAGGAGTTTCCACTTGGTGATCAGCACT3351320.628439986599061No Hit
CACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCT2930520.5495315128153324No Hit
CATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGC2327120.4363818619572009No Hit
GACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTG2311340.43342279419031116No Hit
AGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTG2072790.38868986543292416No Hit
CAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTG1949680.3656042613276133No Hit
CCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGG1890070.35442618594204284No Hit
CTTATTCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACT1796110.33680679383957346No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCT1755300.3291540970355954No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC1606290.30121172137372904No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC1566310.29371466628372556No Hit
ACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTC1564730.29341838446676194No Hit
GTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCC1555100.2916125655443824No Hit
AGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGC1430520.268251306837213No Hit
CGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACC1402970.2630851270540815No Hit
AGGAGCCCCAGCCGTGAGATTCCCAGGAGTTTCCACTTGGTGATCAGCAC1360240.2550723915864515No Hit
GTGACATCCAGATGACCCAGTCTCCCTCCACCCTGTCTGCATCTGTAGGA1352740.25366599055656086No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG1336760.25066941876220733No Hit
TGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTC1318370.2472209234369156No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG1265680.23734048740159083No Hit
TGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCA1220790.22892270843735235No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG1212870.22743754894978785No Hit
ACTTATTCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAAC1199950.22501478877562964No Hit
CCCCAGCCGTGAGATTCCCAGGAGTTTCCACTTGGTGATCAGCACTGAAC1183950.2220144665785297No Hit
GCCCCAGCCGTGAGATTCCCAGGAGTTTCCACTTGGTGATCAGCACTGAA1169020.21921479092836083No Hit
CTCACACATGCCCACCGTGCCCAGCACCTGAACTCCTGGGGGGACCGTCA1158410.21722520227140893No Hit
CCAGCCGTGAGATTCCCAGGAGTTTCCACTTGGTGATCAGCACTGAACAC1077000.20195918789228978No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC1075830.20173978933162684No Hit
CCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTAATCTATAAG1070370.20071592938186647No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGG1018710.19102863908798004No Hit
CCCAGCCGTGAGATTCCCAGGAGTTTCCACTTGGTGATCAGCACTGAACA987410.1851592587899033No Hit
GTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCA975810.18298402519700585No Hit
ATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCT974610.18275900103222337No Hit
GAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT930980.17457749744100645No Hit
CCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAG926800.1737936632670141No Hit
CCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGT914100.17141215752306602No Hit
GGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGC881320.1652652474217575No Hit
CAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT872040.16352506054743954No Hit
CACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGA859900.16124856608038995No Hit
CATCGAGATTCAGCGGCCGTGGATCTGGGACAGAATTCACTCTCACCATC852280.15981966263402111No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCC846030.15864766177577894No Hit
CCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCA840500.15761067541640628No Hit
GTCAGAGTATTACTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAA835630.156697452347664No Hit
GTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT791840.14848594553447608No Hit
GGGAACCCTGGTCACCGTCTCCTCAGCCTCCACCAAGGGCCCATCGGTCT786600.14750334001492585No Hit
GCAGAAACCAGGGAAAGCCCCTAAACTCCTAATCTATAAGGCGTCTACTT785060.14721455900345498No Hit
GCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGG781680.1465807409393176No Hit
ATCGAGATTCAGCGGCCGTGGATCTGGGACAGAATTCACTCTCACCATCA781230.1464963568775242No Hit
CCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGC777530.14580253236944482No Hit
CCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC769270.144253616035192No Hit
CTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGT761880.14286784222040647No Hit
CTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTT760580.14262406604189207No Hit
TGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGT743100.1393462140415604No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC732890.137431633439536No Hit
CAGGAGTTTCCACTTGGTGATCAGCACTGAACACAGACCACCAACCATGG718700.13477072269098297No Hit
TGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAG709120.13297427977546938No Hit
CTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTAATCTATAAGG696770.13065840607958287No Hit
CAGCCGTGAGATTCCCAGGAGTTTCCACTTGGTGATCAGCACTGAACACA681280.1277537191525155No Hit
CATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAG675920.126748611216487No Hit
CAGCACTGAACACAGACCACCAACCATGGAGTTTGGGCTTAGCTGGGTTT671580.12593477382052365No Hit
GCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATA669140.1254772246854659No Hit
CAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAG666370.12495779390509298No Hit
CACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGT656290.12306759092092003No Hit
GTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCT655790.12297383085226066No Hit
GCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCA652900.12243189765540946No Hit
CCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTG646970.1213199032411093No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA644360.12083047568270738No Hit
GTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGGCAA640550.12011602395952295No Hit
CCCTAAACTCCTAATCTATAAGGCGTCTACTTTACAAAGTGGGGTCCCAT639090.11984224455903758No Hit
GAGAGGAGCCCCAGCCGTGAGATTCCCAGGAGTTTCCACTTGGTGATCAG635990.11926093213334946No Hit
CAGCACCTGAACTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAA635900.11924405532099079No Hit
ATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAATTTATT635540.11917654807155603No Hit
CGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCC626800.1175376220713902No Hit
GTCCCATCGAGATTCAGCGGCCGTGGATCTGGGACAGAATTCACTCTCAC614090.11515424112606894No Hit
CCCAGGAGTTTCCACTTGGTGATCAGCACTGAACACAGACCACCAACCAT610840.114544800679783No Hit
AGAGGAGCCCCAGCCGTGAGATTCCCAGGAGTTTCCACTTGGTGATCAGC606400.11371221127008777No Hit
GGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCT602530.11298650833866423No Hit
CGAGATTCAGCGGCCGTGGATCTGGGACAGAATTCACTCTCACCATCAGC595950.11175262583510687No Hit
AACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAG589950.1106275050111944No Hit
GTATAATACTTATTCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA589060.1104606120889807No Hit
GAGGAGCCCCAGCCGTGAGATTCCCAGGAGTTTCCACTTGGTGATCAGCA588950.11043998487387564No Hit
GTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGC588520.11035935121482858No Hit
CCCAGTCTCCCTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATC584410.10958864345044853No Hit
CGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGC583650.10944612814608627No Hit
CTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAG583560.1094292513337276No Hit
AAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGA579310.10863229075012293No Hit
CCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAAAG577550.10830225530844192No Hit
CCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGT569530.10679834380714559No Hit
GGGAAAGCCCCTAAACTCCTAATCTATAAGGCGTCTACTTTACAAAGTGG557030.10445434209066125No Hit
GCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC552260.10355987103565084No Hit
GCCATGAGCTGGTTACGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTAGG546430.10246662863508255No Hit
AATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG541680.1015759079828185No Hit
GTGAGATTCCCAGGAGTTTCCACTTGGTGATCAGCACTGAACACAGACCA540770.10140526465785844No Hit
CTTTACAAAGTGGGGTCCCATCGAGATTCAGCGGCCGTGGATCTGGGACA533300.10000448923208742No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)