FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006144043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006144043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36236481
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGATCCACGGCCGCTGAATCTCGATGGGACCCCACTTTGTAAAGTAG1884430.5200367000316615No Hit
GGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTC1731440.4778168167046905No Hit
GTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAA1284490.3544742658648339No Hit
GTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGT1050680.28995089230656806No Hit
GTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT974230.2688533690674875No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC959010.2646531819687458No Hit
GGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTC927050.25583334099136174No Hit
GTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTG922970.2547074038453127No Hit
CCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACT895270.24706317371159742No Hit
GGCAGTAATAAATTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGA884480.24408551150427657No Hit
CTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGC846140.233505013911257No Hit
GGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTT829410.22888811968248243No Hit
GTGAGAGTGAATTCTGTCCCAGATCCACGGCCGCTGAATCTCGATGGGAC808290.223059739161758No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC803310.22168543352760994No Hit
GGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCA728280.20097978056975233No Hit
GTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCGAGAATAAGTATTA674890.18624600992574306No Hit
CCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGG674320.18608870988328033No Hit
GCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAA646410.17838652710234199No Hit
CCTTGGCCGAACGTCCGAGAATAAGTATTATACTGTTGGCAGTAATAAAT638530.1762119230065414No Hit
GTTGGCAGTAATAAATTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTG633470.17481554017345116No Hit
GCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCGAGA626140.17279271682037778No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC623830.17215523770092356No Hit
GTGAATTCTGTCCCAGATCCACGGCCGCTGAATCTCGATGGGACCCCACT622210.17170817442234526No Hit
GGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCT621350.17147084453371728No Hit
GCCCGATCTCTAGCACAATAATATACGGCTGTGTCGTCGGCTTTCAGGCT611430.16873327186489218No Hit
CCGAGAATAAGTATTATACTGTTGGCAGTAATAAATTGCAAAATCATCAG606930.16749142942439693No Hit
GCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCA583870.16112767682932566No Hit
GTCGTAGTAGCTCCCACCATATAAATTCGCCCGATCTCTAGCACAATAAT567540.15662116859526176No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA559330.15435549605382487No Hit
GGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGT533290.147169367798159No Hit
CTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTT533230.14715280989895238No Hit
CGGCTGTGTCGTCGGCTTTCAGGCTATTCATTTGCAGATAGACGATGCTG530530.14640770443465523No Hit
CCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGG521650.14395713535207794No Hit
CCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT520730.14370324756424335No Hit
GCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACC519350.14332241588249148No Hit
GTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAG487970.13466263459743788No Hit
TGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCT473410.1306445843899688No Hit
CTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCGCTGGGATAGA469040.12943861739775447No Hit
CCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGC468540.12930063490436614No Hit
GTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCGAGAATAAGT462400.12760620988555704No Hit
GTTGGAGTTATCTCTTGAGATGGTGAATCTGTCTTTCACAGACGCGGCGT443470.12238219268587366No Hit
CTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTTCTCGATGG433090.11951767612313126No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG425200.11734031237746291No Hit
AGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAG418340.1154471925681746No Hit
CTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTC418050.11536716272200935No Hit
CTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTT410050.11315944282779555No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT409610.1130380182336138No Hit
GCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCC407150.11235914436614307No Hit
CCGGGATGGGGGCAGGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGG405830.11199487058359779No Hit
GTCGGCTTTCAGGCTATTCATTTGCAGATAGACGATGCTGTTGGAGTTAT403540.11136291076387908No Hit
GTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAG400100.11041359120936717No Hit
CGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCT397190.10961053309784688No Hit
GGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCAC394680.10891786098103731No Hit
GGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTG392590.10834109415867398No Hit
CCCGGGATGGGGGCAGGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTG391080.1079243870286411No Hit
GGCTCTTCTGTGTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCAT390670.10781124138406264No Hit
CCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCGAGAA386470.1066521884396004No Hit
GGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACGGCCGCTGAAT385670.10643141645017902No Hit
CCTGGTTTCTGCTGATACCAGGCCAACCAGCTAGTAATACTCTGACTGGC375410.10360001568584984No Hit
GTCGGAGTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCC371540.10253203118702392No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT369090.10185591696942095No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGA369030.10183935907021435No Hit
CTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGA365370.10082932721861154No Hit
CTTATAGATTAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGG365180.10077689387112396No Hit
CCCACTTTGTAAAGTAGACGCCTTATAGATTAGGAGTTTAGGGGCTTTCC364920.10070514297456201No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT364050.10046505343606627No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)