FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006144044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006144044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36236481
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC5406771.4920792115547865No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT4367721.2053377920444317No Hit
GGAGCCCCAGCCGTGAGATTCCCAGGAGTTTCCACTTGGTGATCAGCACT2791620.7703893763856374No Hit
CACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCT2516840.6945597173191294No Hit
CATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGC1944820.5367022255831079No Hit
CCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGG1736140.4791138521425411No Hit
GACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTG1588140.43827103409958595No Hit
AGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTG1556030.42940979837418536No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCT1440480.397522044152135No Hit
CAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTG1317580.36360594727727563No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC1300860.3589918126983688No Hit
CTTATTCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACT1227360.33870838617027965No Hit
CCCCAGCCGTGAGATTCCCAGGAGTTTCCACTTGGTGATCAGCACTGAAC1201970.3317016351560186No Hit
AGGAGCCCCAGCCGTGAGATTCCCAGGAGTTTCCACTTGGTGATCAGCAC1175550.3244106402053776No Hit
CCAGCCGTGAGATTCCCAGGAGTTTCCACTTGGTGATCAGCACTGAACAC1142470.31528171844280356No Hit
ACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTC1122150.3096741099115005No Hit
GCCCCAGCCGTGAGATTCCCAGGAGTTTCCACTTGGTGATCAGCACTGAA1076490.2970735486152753No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG1020470.2816139900560432No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG1017810.2808799231912172No Hit
AGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGC1006500.2777587591907724No Hit
CCCAGCCGTGAGATTCCCAGGAGTTTCCACTTGGTGATCAGCACTGAACA977020.2696233113805946No Hit
GTGACATCCAGATGACCCAGTCTCCCTCCACCCTGTCTGCATCTGTAGGA902170.24896733212035685No Hit
TGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTC896440.2473860527461262No Hit
GTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCC871580.24052556317485685No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC782450.21592880390344746No Hit
ACTTATTCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAAC766670.21157407641211076No Hit
ATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCT744120.20535106596029565No Hit
CTCACACATGCCCACCGTGCCCAGCACCTGAACTCCTGGGGGGACCGTCA679730.18758168046174242No Hit
CCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGC661430.18253152120372837No Hit
CCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTAATCTATAAG660910.18238801941060448No Hit
CAGCCGTGAGATTCCCAGGAGTTTCCACTTGGTGATCAGCACTGAACACA647500.1786873289379286No Hit
CAGGAGTTTCCACTTGGTGATCAGCACTGAACACAGACCACCAACCATGG643370.17754759354254074No Hit
CATCGAGATTCAGCGGCCGTGGATCTGGGACAGAATTCACTCTCACCATC636120.17554684738840948No Hit
GTCAGAGTATTACTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAA630390.17396556801417887No Hit
TGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCA629390.17368960302740213No Hit
CCCAGGAGTTTCCACTTGGTGATCAGCACTGAACACAGACCACCAACCAT614100.16947009837958601No Hit
CCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGT573140.1581665725212114No Hit
ATCGAGATTCAGCGGCCGTGGATCTGGGACAGAATTCACTCTCACCATCA570240.15736627405955894No Hit
TGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGT568420.1568640177836253No Hit
CTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTT555010.15316332731094945No Hit
CATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAG549640.15168139533195843No Hit
CAGCACTGAACACAGACCACCAACCATGGAGTTTGGGCTTAGCTGGGTTT543860.15008631770838896No Hit
CGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACC537160.1482373522969849No Hit
AGAGGAGCCCCAGCCGTGAGATTCCCAGGAGTTTCCACTTGGTGATCAGC534120.14739841873718365No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC532070.14683269051429138No Hit
CCCTAAACTCCTAATCTATAAGGCGTCTACTTTACAAAGTGGGGTCCCAT511910.14126923638087263No Hit
CAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT504140.13912498843361748No Hit
GTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGGCAA491660.13568094539864398No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG489850.1351814487725781No Hit
GAGGAGCCCCAGCCGTGAGATTCCCAGGAGTTTCCACTTGGTGATCAGCA487360.13449429595550408No Hit
CCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC479840.1324190392549431No Hit
GAGAGGAGCCCCAGCCGTGAGATTCCCAGGAGTTTCCACTTGGTGATCAG479630.13236108660772No Hit
CCAGGAGTTTCCACTTGGTGATCAGCACTGAACACAGACCACCAACCATG461780.12743511159375548No Hit
GCAGAAACCAGGGAAAGCCCCTAAACTCCTAATCTATAAGGCGTCTACTT459960.12693285531782184No Hit
CCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAG447850.12359091932795571No Hit
ATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAATTTATT447580.123516408781526No Hit
CTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGT444120.12256156992727853No Hit
CCCAGTCTCCCTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATC435430.12016343419218879No Hit
GGGAACCCTGGTCACCGTCTCCTCAGCCTCCACCAAGGGCCCATCGGTCT433320.11958114807008992No Hit
GAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT423650.116912566647959No Hit
CCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTG423620.1169042876983557No Hit
GTGAGATTCCCAGGAGTTTCCACTTGGTGATCAGCACTGAACACAGACCA422490.11659244726329801No Hit
CGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCC414420.11436540982000985No Hit
CTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTAATCTATAAGG409430.11298834453599399No Hit
GAGCCCCAGCCGTGAGATTCCCAGGAGTTTCCACTTGGTGATCAGCACTG398500.1099720472305244No Hit
GTCCCATCGAGATTCAGCGGCCGTGGATCTGGGACAGAATTCACTCTCAC397670.10974299629149971No Hit
GTATAATACTTATTCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA394690.10892062063090509No Hit
CAACACTTTACGCCGCGTCTGTGAAAGACAGATTCACCATCTCAAGAGAT384700.1061637304130056No Hit
CCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCA379680.10477838617938647No Hit
CCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAAAG379260.10466248088494023No Hit
CCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGT374270.10328541560092439No Hit
CGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGC373890.10318054890594923No Hit
CTTTACAAAGTGGGGTCCCATCGAGATTCAGCGGCCGTGGATCTGGGACA373150.10297633481573445No Hit
GCCATGAGCTGGTTACGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTAGG373070.1029542576167923No Hit
CCTAAACTCCTAATCTATAAGGCGTCTACTTTACAAAGTGGGGTCCCATC367820.10150544143621451No Hit
ATTCCCAGGAGTTTCCACTTGGTGATCAGCACTGAACACAGACCACCAAC367770.10149164318687567No Hit
GCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGG366320.10109149395604942No Hit
CGAGATTCAGCGGCCGTGGATCTGGGACAGAATTCACTCTCACCATCAGC363640.10035190779148781No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)