FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006144239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006144239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435961
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTGGCGATCTCGTAT194034.450627464383282TruSeq Adapter, Index 15 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTGGCGATCGCGTAT89642.0561472241783094TruSeq Adapter, Index 15 (97% over 37bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25110.5759689513511529No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA14860.340856177502116No Hit
CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT6380.1463433655762786No Hit
GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA6060.13900325946586964No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTGGCGATCTCGGAT5970.13693885462231714TruSeq Adapter, Index 15 (97% over 37bp)
CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT5860.13441569314686405No Hit
AGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAG5010.11491853629109025No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTGGCGATCGCGGAT4920.11285413144753774TruSeq Adapter, Index 15 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTGGCGATCTCGTTT4590.1052846470211785TruSeq Adapter, Index 15 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)