FastQCFastQC Report
Wed 24 Aug 2022
EGAF00006144245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006144245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1566845
Sequences flagged as poor quality0
Sequence length32-251
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCACAAGAGTGCTACTCTCCTCGCTCCGGGCCCATAACACTTGGGGGT308301.9676483634309712No Hit
GATATTGATTTCACGGAGGATGGTGGTCAAGGGACCCCTATCTGAGGGGG101870.6501600349747423No Hit
CCGCACAAGAGTGCTACTCTCCTCTCGTCCCCATGGATGACCCCCCTCAG68840.439354243719066No Hit
GATATTGATTTCACGGAGGATGGTGTTAGGGTTCTTTGTTTTTGGGGTTT62340.39786960420462775No Hit
GCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGCCATAAAG42040.2683098838749206No Hit
ATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGG41730.2663313856826936No Hit
ATTGATTTCACGGAGGATGGTGGTCAAGGGACCCCTATCTGAGGGGGGTC35140.22427234346728617No Hit
ATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTA33080.21112490386732571No Hit
CTTGACCACCATCCTCCGTGAAATCAATATCCCGCACAAGAGTGCTACTC32020.2043597165003558No Hit
GATAGGGGTCCCTTGACCACCATCCTCCGTGAAATCAATATCCCGCACAA30640.1955522084188289No Hit
ATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCCCGCA27560.17589487154121816No Hit
ATGGATGACCCCCCTCAGATAGGGGTCCCTTGACCACCATCCTCCGTGAA26480.16900203912958844No Hit
ATTGCGGGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTG26070.16638531571406234No Hit
GACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTG25150.16051364365971107No Hit
CTCTTGTGCGGGATATTGATTTCACGGAGGATGGTGGTCAAGGGACCCCT24800.1582798553781644No Hit
ATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTC23740.15151466801119445No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22170.14149453200539938No Hit
ATTTAGGCTTTATGGCCCTGAAGTAGGAACCAGATGTCGGATACAGTTCA22040.1406648392151106No Hit
GTGCTACTCTCCTCGCTCCGGGCCCATAACACTTGGGGGTAGCTAAAGTG21670.13830340588890414No Hit
ATCCATGGGGACGAGAGGAGAGTAGCACTCTTGTGCGGGATATTGATTTC21450.13689931039764622No Hit
ATGGGGACGAGAGGAGAGTAGCACTCTTGTGCGGGATATTGATTTCACGG21120.13479316716075937No Hit
ATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATC20640.1317296860889239No Hit
GTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGCCATAAAGCCTAAA20310.12962354285203706No Hit
ATCGCGTGCATACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCC20010.12770886718213992No Hit
ATCTGGTTCCTACTTCAGGGCCATAAAGCCTAAATAGCCCACACGTTCCC19930.12719828700350067No Hit
ATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGCTTTATGG19750.12604948160156237No Hit
CTTCAGGGCCATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGAC19220.12266688791807742No Hit
GTAGCACTCTTGTGCGGGATATTGATTTCACGGAGGATGGTGGTCAAGGG18900.12062456720352045No Hit
ATTTTCGTCTGGGGGGTATGCACGCGATAGCATTGCGGGACGCTGGAGCC18740.11960340684624196No Hit
GTGCTACTCTCCTCTCGTCCCCATGGATGACCCCCCTCAGATAGGGGTCC18300.11679521586372614No Hit
ATTTCACGGAGGATGGTGGTCAAGGGACCCCTATCTGAGGGGGGTCATCC18150.11583787802877758No Hit
GTGCATACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAG17270.11022149606374594No Hit
ATCTGAGGGGGGTCATCCATGGGGACGAGAGGAGAGTAGCACTCTTGTGC17120.10926415822879736No Hit
ACTCTCCTCGCTCCGGGCCCATAACACTTGGGGGTAGCTAAAGTGAACTG17040.10875357805015813No Hit
GAGGAGAGTAGCACTCTTGTGCGGGATATTGATTTCACGGAGGATGGTGG16480.10517951679968345No Hit
GATAAATAATAGGATGGGGCAGGAATCAAAGACAGATACTGCGACATAGG16350.10434982400939469No Hit
ATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGC16170.10320101860745638No Hit
ACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCC16110.10281808347347697No Hit
CTCGTCCCCATGGATGACCCCCCTCAGATAGGGGTCCCTTGACCACCATC16070.10256279338415734No Hit
GTGAAATCAATATCCCGCACAAGAGTGCTACTCTCCTCTCGTCCCCATGG15970.10192456816085829No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)