FastQCFastQC Report
Tue 23 Aug 2022
EGAF00006144246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006144246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104384
Sequences flagged as poor quality0
Sequence length32-251
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT94849.085683629675046TruSeq Adapter, Index 13 (97% over 38bp)
AAGGATTATGGATGCGGTTGCTTGCGTGAGGAAATACTTGATGGCAGCTT40453.875114960147149No Hit
TGGCTCCTTTAACCTCTCCACCCTTATCACAACACAAGAACACCTCTGAT32003.065603923973023No Hit
GGCTCCTTTAACCTCTCCACCCTTATCACAACACAAGAACACCTCTGATT8860.8487890864500306No Hit
GTTGAAGCCTGAGACTAGTTCGGACTCCCCTTCGGCAAGGTCGAAGGGGG5620.5383966891477622No Hit
ATGAAGAATAGGGCGAAGGGGCCTGCGGCGTATTCGATGTTGAAGCCTGA5120.4904966278356836No Hit
GTTCGGACTCCCCTTCGGCAAGGTCGAAGGGGGTTCGGTTGGTCTCTGCT4570.4378065603923973No Hit
GTTTATTATAATAATGTTTGTGTATTCGGCTATGAAGAATAGGGCGAAGG4290.4109825260576333No Hit
GTTCGAATTCATAAGAACAGGGAGGTTAGAAGTAGGGTCTTGGTGACAAA4250.4071505211526671No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4020.385116492949111No Hit
ATCACAGCGCTAAGCTCGCACTGATTTTTTACCTGAGTAGGCCTAGAAAT3580.3429644389944819No Hit
ATCATGACCCTTGGCCATAATATGATTTATCTCCACACTAGCAGAGACCA3470.3324264255058247No Hit
GTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATAAGAACAGG3370.32284641324340896No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC3290.31518240343347637No Hit
GTCATATGTTGTTCCTAGGAAGATTGTAGTGGTGAGGGTGTTTATTATAA3190.3056023911710607No Hit
ATCCTAAAGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAA3170.30368638871857756No Hit
GTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGCTC3170.30368638871857756No Hit
ATTACAATCTCCAGCATTCCCCCTCAAACCTAAGAAATATGTCTGATAAA3030.29027437155119556No Hit
ATCGGGCCCATACCCCGAAAATGTTGGTTATACCCTTCCCGTACTAATTA2950.282610361741263No Hit
ATTCGGCTATGAAGAATAGGGCGAAGGGGCCTGCGGCGTATTCGATGTTG2830.27111434702636417No Hit
GTTCAGGGGAGAGTGCGTCATATGTTGTTCCTAGGAAGATTGTAGTGGTG2720.26057633353770693No Hit
ATACCCCGAAAATGTTGGTTATACCCTTCCCGTACTAATTAATCCCCTGG2680.25674432863274066No Hit
GAATAGGGCGAAGGGGCCTGCGGCGTATTCGATGTTGAAGCCTGAGACTA2650.25387032495401596No Hit
GTATTCGATGTTGAAGCCTGAGACTAGTTCGGACTCCCCTTCGGCAAGGT2620.2509963212752912No Hit
GTCCGAACTAGTCTCAGGCTTCAACATCGAATACGCCGCAGGCCCCTTCG2600.2490803188228081No Hit
ATGCTGTTCGAATTCATAAGAACAGGGAGGTTAGAAGTAGGGTCTTGGTG2580.24716431637032496No Hit
GAAAATGTTGGTTATACCCTTCCCGTACTAATTAATCCCCTGGCCCAACC2540.2433323114653587No Hit
ATTCGATGTTGAAGCCTGAGACTAGTTCGGACTCCCCTTCGGCAAGGTCG2540.2433323114653587No Hit
CTACAATCTTCCTAGGAACAACATATGACGCACTCTCCCCTGAACTCTAC2490.2385423053341508No Hit
ATGGAGACATGTCATATAAGTAATGCTAGGGTGAGTGGTAGGAAGTTTTT2440.233752299202943No Hit
GACAAAATATGTTGTGTAGAGTTCAGGGGAGAGTGCGTCATATGTTGTTC2400.2299202942979767No Hit
GTGCGTCATATGTTGTTCCTAGGAAGATTGTAGTGGTGAGGGTGTTTATT2400.2299202942979767No Hit
GGTAAAAAATCAGTGCGAGCTTAGCGCTGTGATGAGTGTGCCTGCAAAGA2370.227046290619252No Hit
GTCTCAGGCTTCAACATCGAATACGCCGCAGGCCCCTTCGCCCTATTCTT2280.21842427958307786No Hit
GGTTAGAAGTAGGGTCTTGGTGACAAAATATGTTGTGTAGAGTTCAGGGG2230.21363427345187No Hit
GTGATAGGTGGCACGGAGAATTTTGGATTCTCAGGGATGGGTTCGATTCT2190.20980226854690376No Hit
GTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATAAG2180.2088442673206622No Hit
GAAGGGGCCTGCGGCGTATTCGATGTTGAAGCCTGAGACTAGTTCGGACT2130.2040542611894543No Hit
ATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAAGCTATCGGGCCCAT2080.1992642550582465No Hit
ACTCTCCCCTGAACTCTACACAACATATTTTGTCACCAAGACCCTACTTC2070.1983062538320049No Hit
CTGCGGCGTATTCGATGTTGAAGCCTGAGACTAGTTCGGACTCCCCTTCG2070.1983062538320049No Hit
ATAAAAGCTAGCATGTTTATTTCTAGGCCTACTCAGGTAAAAAATCAGTG2050.19639025137952176No Hit
ATAACCAACATTTTCGGGGTATGGGCCCGATAGCTTATTTAGCTGACCTT2010.1925582464745555No Hit
GTAAATAATAGGAGCTTAAACCCCCTTATTTCTAGGACTATGAGAATCGA1980.1896842427958308No Hit
CTTCGACCTTGCCGAAGGGGAGTCCGAACTAGTCTCAGGCTTCAACATCG1980.1896842427958308No Hit
CTCAAACCTAAGAAATATGTCTGATAAAAGAGTTACTTTGATAGAGTAAA1960.18776824034334763No Hit
ATAATATGATTTATCTCCACACTAGCAGAGACCAACCGAACCCCCTTCGA1950.1868102391171061No Hit
ATGCTGGAGATTGTAATGGGTATGGAGACATGTCATATAAGTAATGCTAG1940.1858522378908645No Hit
CTTGGTGACAAAATATGTTGTGTAGAGTTCAGGGGAGAGTGCGTCATATG1920.18393623543838136No Hit
GGCACACTCATCACAGCGCTAAGCTCGCACTGATTTTTTACCTGAGTAGG1900.18202023298589823No Hit
ATCTTTGCAGGCACACTCATCACAGCGCTAAGCTCGCACTGATTTTTTAC1880.1801042305334151No Hit
GATAGCTTATTTAGCTGACCTTACTTTAGGATGGGGTGTGATAGGTGGCA1860.17818822808093193No Hit
ATGAAAAAACTTCCTACCACTCACCCTAGCATTACTTATATGACATGTCT1860.17818822808093193No Hit
GCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGTTATACCCTTC1800.17244022072348253No Hit
GTATTCGGCTATGAAGAATAGGGCGAAGGGGCCTGCGGCGTATTCGATGT1790.17148221949724096No Hit
GGTTTGAGGGGGAATGCTGGAGATTGTAATGGGTATGGAGACATGTCATA1750.1676502145922747No Hit
ATCGGGGGTATGCTGTTCGAATTCATAAGAACAGGGAGGTTAGAAGTAGG1740.1666922133660331No Hit
ATCAAAGTAACTCTTTTATCAGACATATTTCTTAGGTTTGAGGGGGAATG1730.16573421213979153No Hit
ATTCTTCATAGCCGAATACACAAACATTATTATAATAAACACCCTCACCA1720.16477621091355No Hit
GAATTTTGGATTCTCAGGGATGGGTTCGATTCTCATAGTCCTAGAAATAA1720.16477621091355No Hit
GCTTAAACCCCCTTATTTCTAGGACTATGAGAATCGAACCCATCCCTGAG1710.1638182096873084No Hit
ATCCCTGAGAATCCAAAATTCTCCGTGCCACCTATCACACCCCATCCTAA1700.16286020846106683No Hit
GTGCGAGCTTAGCGCTGTGATGAGTGTGCCTGCAAAGATGGTAGAGTAGA1680.1609442060085837No Hit
GATGACGGGTTGGGCCAGGGGATTAATTAGTACGGGAAGGGTATAACCAA1680.1609442060085837No Hit
CTTCGGCAAGGTCGAAGGGGGTTCGGTTGGTCTCTGCTAGTGTGGAGATA1680.1609442060085837No Hit
CCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCA1640.1571122011036174No Hit
CTTCTAACCTCCCTGTTCTTATGAATTCGAACAGCATACCCCCGATTCCG1640.1571122011036174No Hit
ATATTTCTTAGGTTTGAGGGGGAATGCTGGAGATTGTAATGGGTATGGAG1610.1542381974248927No Hit
ATAGGGCGAAGGGGCCTGCGGCGTATTCGATGTTGAAGCCTGAGACTAGT1560.14944819129368486No Hit
ATACACAAACATTATTATAATAAACACCCTCACCACTACAATCTTCCTAG1550.1484901900674433No Hit
AACCCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTA1550.1484901900674433No Hit
ATGGGCCCGATAGCTTATTTAGCTGACCTTACTTTAGGATGGGGTGTGAT1540.14753218884120173No Hit
ATCCAAAATTCTCCGTGCCACCTATCACACCCCATCCTAAAGTAAGGTCA1540.14753218884120173No Hit
GATTCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCA1520.1456161863887186No Hit
GCTAAGCTCGCACTGATTTTTTACCTGAGTAGGCCTAGAAATAAACATGC1510.144658185162477No Hit
GTGATGAGTGTGCCTGCAAAGATGGTAGAGTAGATGACGGGTTGGGCCAG1510.144658185162477No Hit
ACGGAGAATTTTGGATTCTCAGGGATGGGTTCGATTCTCATAGTCCTAGA1480.1417841814837523No Hit
ATCGAATACGCCGCAGGCCCCTTCGCCCTATTCTTCATAGCCGAATACAC1460.13986817903126916No Hit
GTGGTGAGGGTGTTTATTATAATAATGTTTGTGTATTCGGCTATGAAGAA1420.1360361741263029No Hit
CTTCGCCCTATTCTTCATAGCCGAATACACAAACATTATTATAATAAACA1420.1360361741263029No Hit
ATCGAACCCATCCCTGAGAATCCAAAATTCTCCGTGCCACCTATCACACC1410.1350781729000613No Hit
ATCACAACACAAGAACACCTCTGATTACTCCTGCCATCATGACCCTTGGC1410.1350781729000613No Hit
CTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGCTCGCACTGAT1400.13412017167381973No Hit
ACCGAACCCCCTTCGACCTTGCCGAAGGGGAGTCCGAACTAGTCTCAGGC1400.13412017167381973No Hit
GTTCTTATGAATTCGAACAGCATACCCCCGATTCCGCTACGACCAACTCA1400.13412017167381973No Hit
CTGCAAAGATGGTAGAGTAGATGACGGGTTGGGCCAGGGGATTAATTAGT1390.1331621704475782No Hit
ACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGCTCGCACTGA1390.1331621704475782No Hit
CTGAGAATCCAAAATTCTCCGTGCCACCTATCACACCCCATCCTAAAGTA1380.1322041692213366No Hit
GATTGTAATGGGTATGGAGACATGTCATATAAGTAATGCTAGGGTGAGTG1370.13124616799509503No Hit
ATTCATAAGAACAGGGAGGTTAGAAGTAGGGTCTTGGTGACAAAATATGT1350.1293301655426119No Hit
GTAGATGACGGGTTGGGCCAGGGGATTAATTAGTACGGGAAGGGTATAAC1350.1293301655426119No Hit
GTGACAAAATATGTTGTGTAGAGTTCAGGGGAGAGTGCGTCATATGTTGT1330.12741416309012876No Hit
GTACGGGAAGGGTATAACCAACATTTTCGGGGTATGGGCCCGATAGCTTA1330.12741416309012876No Hit
GCTTCTGTGGAACGAGGGTTTATTTTTTTGGTTAGAACTGGAATAAAAGC1320.1264561618638872No Hit
GTACTAATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGC1320.1264561618638872No Hit
ACTCATCACAGCGCTAAGCTCGCACTGATTTTTTACCTGAGTAGGCCTAG1300.12454015941140405No Hit
GTAGAGTTCAGGGGAGAGTGCGTCATATGTTGTTCCTAGGAAGATTGTAG1300.12454015941140405No Hit
GTAGGGTCTTGGTGACAAAATATGTTGTGTAGAGTTCAGGGGAGAGTGCG1300.12454015941140405No Hit
GGGCGAAGGGGCCTGCGGCGTATTCGATGTTGAAGCCTGAGACTAGTTCG1290.12358215818516248No Hit
GAAGTAGGGTCTTGGTGACAAAATATGTTGTGTAGAGTTCAGGGGAGAGT1280.1226241569589209No Hit
ACACTCATCACAGCGCTAAGCTCGCACTGATTTTTTACCTGAGTAGGCCT1260.12070815450643778No Hit
CTATGAGAATCGAACCCATCCCTGAGAATCCAAAATTCTCCGTGCCACCT1260.12070815450643778No Hit
CCTCAAACCTAAGAAATATGTCTGATAAAAGAGTTACTTTGATAGAGTAA1230.11783415082771305No Hit
CTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCAC1230.11783415082771305No Hit
ATTATTTACTCTATCAAAGTAACTCTTTTATCAGACATATTTCTTAGGTT1230.11783415082771305No Hit
ATATGACGCACTCTCCCCTGAACTCTACACAACATATTTTGTCACCAAGA1230.11783415082771305No Hit
CTGAACTCTACACAACATATTTTGTCACCAAGACCCTACTTCTAACCTCC1220.1168761496014715No Hit
GATTGTAGTGGTGAGGGTGTTTATTATAATAATGTTTGTGTATTCGGCTA1220.1168761496014715No Hit
GAATACACAAACATTATTATAATAAACACCCTCACCACTACAATCTTCCT1210.11591814837522993No Hit
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGG1210.11591814837522993No Hit
CTCCACCCTTATCACAACACAAGAACACCTCTGATTACTCCTGCCATCAT1210.11591814837522993No Hit
GAGACCAACCGAACCCCCTTCGACCTTGCCGAAGGGGAGTCCGAACTAGT1210.11591814837522993No Hit
CTATTCTTCATAGCCGAATACACAAACATTATTATAATAAACACCCTCAC1200.11496014714898835No Hit
GTTAGAAGTAGGGTCTTGGTGACAAAATATGTTGTGTAGAGTTCAGGGGA1200.11496014714898835No Hit
GTGGAACGAGGGTTTATTTTTTTGGTTAGAACTGGAATAAAAGCTAGCAT1200.11496014714898835No Hit
TTCAACATCGAATACGCCGCAGGCCCCTTCGCCCTATTCTTCATAGCCGA1190.11400214592274678No Hit
ACTAATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAG1180.1130441446965052No Hit
ACTGATTTTTTACCTGAGTAGGCCTAGAAATAAACATGCTAGCTTTTATT1170.11208614347026365No Hit
GTAGCGGAATCGGGGGTATGCTGTTCGAATTCATAAGAACAGGGAGGTTA1170.11208614347026365No Hit
ATACGCCGCAGGCCCCTTCGCCCTATTCTTCATAGCCGAATACACAAACA1170.11208614347026365No Hit
ATATTTTGTCACCAAGACCCTACTTCTAACCTCCCTGTTCTTATGAATTC1170.11208614347026365No Hit
GTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATAAGAACAGGGAGG1170.11208614347026365No Hit
GTCTTGGTGACAAAATATGTTGTGTAGAGTTCAGGGGAGAGTGCGTCATA1170.11208614347026365No Hit
ATTACTTATATGACATGTCTCCATACCCATTACAATCTCCAGCATTCCCC1160.11112814224402208No Hit
GTTTTTTCATAGGAGGTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATG1160.11112814224402208No Hit
ATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGCTCGCA1150.1101701410177805No Hit
GTGTGCCTGCAAAGATGGTAGAGTAGATGACGGGTTGGGCCAGGGGATTA1150.1101701410177805No Hit
ATGAGAATCGAACCCATCCCTGAGAATCCAAAATTCTCCGTGCCACCTAT1140.10921213979153893No Hit
ATCTTCCTAGGAACAACATATGACGCACTCTCCCCTGAACTCTACACAAC1140.10921213979153893No Hit
ATTCGAACAGCATACCCCCGATTCCGCTACGACCAACTCATACACCTCCT1140.10921213979153893No Hit
GTTGGGCCAGGGGATTAATTAGTACGGGAAGGGTATAACCAACATTTTCG1130.10825413856529735No Hit
ATTAGTACGGGAAGGGTATAACCAACATTTTCGGGGTATGGGCCCGATAG1130.10825413856529735No Hit
CTTTAACCTCTCCACCCTTATCACAACACAAGAACACCTCTGATTACTCC1120.1072961373390558No Hit
GACATGTCATATAAGTAATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATA1100.10538013488657265No Hit
GCCTGAGACTAGTTCGGACTCCCCTTCGGCAAGGTCGAAGGGGGTTCGGT1100.10538013488657265No Hit
CTTATTTCTAGGACTATGAGAATCGAACCCATCCCTGAGAATCCAAAATT1090.1044221336603311No Hit
ATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATAAGAA1080.1034641324340895No Hit
GTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGGTCGTAGCGGAAT1080.1034641324340895No Hit
ACTACAATCTTCCTAGGAACAACATATGACGCACTCTCCCCTGAACTCTA1070.10250613120784795No Hit
AGGATTATGGATGCGGTTGCTTGCGTGAGGAAATACTTGATGGCAGCTTC1070.10250613120784795No Hit
CCTTCGACCTTGCCGAAGGGGAGTCCGAACTAGTCTCAGGCTTCAACATC1070.10250613120784795No Hit
CTGATTTTTTACCTGAGTAGGCCTAGAAATAAACATGCTAGCTTTTATTC1070.10250613120784795No Hit
GTCCTAGAAATAAGGGGGTTTAAGCTCCTATTATTTACTCTATCAAAGTA1070.10250613120784795No Hit
CCGAAAATGTTGGTTATACCCTTCCCGTACTAATTAATCCCCTGGCCCAA1060.10154812998160638No Hit
GAACTAGTCTCAGGCTTCAACATCGAATACGCCGCAGGCCCCTTCGCCCT1060.10154812998160638No Hit
GTCGAAGGGGGTTCGGTTGGTCTCTGCTAGTGTGGAGATAAATCATATTA1050.1005901287553648No Hit
GACCAACCGAACCCCCTTCGACCTTGCCGAAGGGGAGTCCGAACTAGTCT1050.1005901287553648No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)