FastQCFastQC Report
Tue 23 Aug 2022
EGAF00006144247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006144247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104384
Sequences flagged as poor quality0
Sequence length32-251
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGATTATGGATGCGGTTGCTTGCGTGAGGAAATACTTGATGGCAGCTT44444.257357449417535No Hit
TGGCTCCTTTAACCTCTCCACCCTTATCACAACACAAGAACACCTCTGAT34783.331928264868179No Hit
GGCTCCTTTAACCTCTCCACCCTTATCACAACACAAGAACACCTCTGATT18841.8048743102391172No Hit
GTTGAAGCCTGAGACTAGTTCGGACTCCCCTTCGGCAAGGTCGAAGGGGG4400.4215205395462906No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3900.3736204782342122No Hit
GTTCGAATTCATAAGAACAGGGAGGTTAGAAGTAGGGTCTTGGTGACAAA3900.3736204782342122No Hit
GTTCGGACTCCCCTTCGGCAAGGTCGAAGGGGGTTCGGTTGGTCTCTGCT3590.3439224402207235No Hit
ATGAAGAATAGGGCGAAGGGGCCTGCGGCGTATTCGATGTTGAAGCCTGA3540.33913243408951566No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC3050.2921903740036787No Hit
GTTTATTATAATAATGTTTGTGTATTCGGCTATGAAGAATAGGGCGAAGG2980.2854843654199878No Hit
CTACAATCTTCCTAGGAACAACATATGACGCACTCTCCCCTGAACTCTAC2870.2749463519313305No Hit
ATCACAGCGCTAAGCTCGCACTGATTTTTTACCTGAGTAGGCCTAGAAAT2830.27111434702636417No Hit
GTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATAAGAACAGG2690.2577023298589822No Hit
GTCTCAGGCTTCAACATCGAATACGCCGCAGGCCCCTTCGCCCTATTCTT2660.2548283261802575No Hit
GTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGCTC2580.24716431637032496No Hit
GTCATATGTTGTTCCTAGGAAGATTGTAGTGGTGAGGGTGTTTATTATAA2580.24716431637032496No Hit
ATCGGGCCCATACCCCGAAAATGTTGGTTATACCCTTCCCGTACTAATTA2580.24716431637032496No Hit
ATACCCCGAAAATGTTGGTTATACCCTTCCCGTACTAATTAATCCCCTGG2560.2452483139178418No Hit
GTCCGAACTAGTCTCAGGCTTCAACATCGAATACGCCGCAGGCCCCTTCG2410.2308782955242183No Hit
ATCCTAAAGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAA2410.2308782955242183No Hit
ATCATGACCCTTGGCCATAATATGATTTATCTCCACACTAGCAGAGACCA2370.227046290619252No Hit
ATGGAGACATGTCATATAAGTAATGCTAGGGTGAGTGGTAGGAAGTTTTT2360.2260882893930104No Hit
GTTCAGGGGAGAGTGCGTCATATGTTGTTCCTAGGAAGATTGTAGTGGTG2350.22513028816676886No Hit
ATTCGGCTATGAAGAATAGGGCGAAGGGGCCTGCGGCGTATTCGATGTTG2190.20980226854690376No Hit
GGTAAAAAATCAGTGCGAGCTTAGCGCTGTGATGAGTGTGCCTGCAAAGA2150.20597026364193746No Hit
GACAAAATATGTTGTGTAGAGTTCAGGGGAGAGTGCGTCATATGTTGTTC2140.2050122624156959No Hit
CTTCGACCTTGCCGAAGGGGAGTCCGAACTAGTCTCAGGCTTCAACATCG2140.2050122624156959No Hit
ATTACAATCTCCAGCATTCCCCCTCAAACCTAAGAAATATGTCTGATAAA2100.2011802575107296No Hit
GATAGCTTATTTAGCTGACCTTACTTTAGGATGGGGTGTGATAGGTGGCA2080.1992642550582465No Hit
ATGCTGGAGATTGTAATGGGTATGGAGACATGTCATATAAGTAATGCTAG2080.1992642550582465No Hit
GAATAGGGCGAAGGGGCCTGCGGCGTATTCGATGTTGAAGCCTGAGACTA2080.1992642550582465No Hit
GTGATAGGTGGCACGGAGAATTTTGGATTCTCAGGGATGGGTTCGATTCT2050.19639025137952176No Hit
ATAACCAACATTTTCGGGGTATGGGCCCGATAGCTTATTTAGCTGACCTT2000.19160024524831393No Hit
GTGCGTCATATGTTGTTCCTAGGAAGATTGTAGTGGTGAGGGTGTTTATT1970.1887262415695892No Hit
ATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAAGCTATCGGGCCCAT1900.18202023298589823No Hit
GTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATAAG1890.18106223175965663No Hit
ATGAAAAAACTTCCTACCACTCACCCTAGCATTACTTATATGACATGTCT1890.18106223175965663No Hit
GTATTCGATGTTGAAGCCTGAGACTAGTTCGGACTCCCCTTCGGCAAGGT1850.1772302268546904No Hit
CTTGGTGACAAAATATGTTGTGTAGAGTTCAGGGGAGAGTGCGTCATATG1840.1762722256284488No Hit
ATGCTGTTCGAATTCATAAGAACAGGGAGGTTAGAAGTAGGGTCTTGGTG1830.17531422440220723No Hit
ATTCGATGTTGAAGCCTGAGACTAGTTCGGACTCCCCTTCGGCAAGGTCG1810.1733982219497241No Hit
GCTTAAACCCCCTTATTTCTAGGACTATGAGAATCGAACCCATCCCTGAG1810.1733982219497241No Hit
GGTTAGAAGTAGGGTCTTGGTGACAAAATATGTTGTGTAGAGTTCAGGGG1810.1733982219497241No Hit
ACTCTCCCCTGAACTCTACACAACATATTTTGTCACCAAGACCCTACTTC1800.17244022072348253No Hit
ATAATATGATTTATCTCCACACTAGCAGAGACCAACCGAACCCCCTTCGA1770.16956621704475783No Hit
ATCTTTGCAGGCACACTCATCACAGCGCTAAGCTCGCACTGATTTTTTAC1760.16860821581851623No Hit
GAATTTTGGATTCTCAGGGATGGGTTCGATTCTCATAGTCCTAGAAATAA1740.1666922133660331No Hit
GAAGGGGCCTGCGGCGTATTCGATGTTGAAGCCTGAGACTAGTTCGGACT1720.16477621091355No Hit
GAAAATGTTGGTTATACCCTTCCCGTACTAATTAATCCCCTGGCCCAACC1700.16286020846106683No Hit
GGTTTGAGGGGGAATGCTGGAGATTGTAATGGGTATGGAGACATGTCATA1700.16286020846106683No Hit
CTCAAACCTAAGAAATATGTCTGATAAAAGAGTTACTTTGATAGAGTAAA1690.16190220723482526No Hit
GATGACGGGTTGGGCCAGGGGATTAATTAGTACGGGAAGGGTATAACCAA1690.16190220723482526No Hit
ATCGAATACGCCGCAGGCCCCTTCGCCCTATTCTTCATAGCCGAATACAC1680.1609442060085837No Hit
GTAAATAATAGGAGCTTAAACCCCCTTATTTCTAGGACTATGAGAATCGA1680.1609442060085837No Hit
ATCAAAGTAACTCTTTTATCAGACATATTTCTTAGGTTTGAGGGGGAATG1670.15998620478234213No Hit
ATTCTTCATAGCCGAATACACAAACATTATTATAATAAACACCCTCACCA1660.15902820355610053No Hit
ATAAAAGCTAGCATGTTTATTTCTAGGCCTACTCAGGTAAAAAATCAGTG1620.1551961986511343No Hit
CTTCGCCCTATTCTTCATAGCCGAATACACAAACATTATTATAATAAACA1600.15328019619865113No Hit
GAGACCAACCGAACCCCCTTCGACCTTGCCGAAGGGGAGTCCGAACTAGT1570.15040619251992643No Hit
CTGCGGCGTATTCGATGTTGAAGCCTGAGACTAGTTCGGACTCCCCTTCG1570.15040619251992643No Hit
GTATTCGGCTATGAAGAATAGGGCGAAGGGGCCTGCGGCGTATTCGATGT1570.15040619251992643No Hit
ATGGGCCCGATAGCTTATTTAGCTGACCTTACTTTAGGATGGGGTGTGAT1550.1484901900674433No Hit
ATATTTCTTAGGTTTGAGGGGGAATGCTGGAGATTGTAATGGGTATGGAG1540.14753218884120173No Hit
GTACGGGAAGGGTATAACCAACATTTTCGGGGTATGGGCCCGATAGCTTA1530.14657418761496013No Hit
CCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCA1500.14370018393623543No Hit
ACCGAACCCCCTTCGACCTTGCCGAAGGGGAGTCCGAACTAGTCTCAGGC1500.14370018393623543No Hit
ATCACAACACAAGAACACCTCTGATTACTCCTGCCATCATGACCCTTGGC1490.1427421827099939No Hit
GCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGTTATACCCTTC1470.14082618025751073No Hit
CTCCACCCTTATCACAACACAAGAACACCTCTGATTACTCCTGCCATCAT1460.13986817903126916No Hit
ATCCCTGAGAATCCAAAATTCTCCGTGCCACCTATCACACCCCATCCTAA1450.1389101778050276No Hit
CTGCAAAGATGGTAGAGTAGATGACGGGTTGGGCCAGGGGATTAATTAGT1450.1389101778050276No Hit
GGCACACTCATCACAGCGCTAAGCTCGCACTGATTTTTTACCTGAGTAGG1430.13699417535254443No Hit
CTATTCTTCATAGCCGAATACACAAACATTATTATAATAAACACCCTCAC1420.1360361741263029No Hit
GTGCGAGCTTAGCGCTGTGATGAGTGTGCCTGCAAAGATGGTAGAGTAGA1410.1350781729000613No Hit
CTTTAACCTCTCCACCCTTATCACAACACAAGAACACCTCTGATTACTCC1390.1331621704475782No Hit
GAATACACAAACATTATTATAATAAACACCCTCACCACTACAATCTTCCT1380.1322041692213366No Hit
GATTGTAATGGGTATGGAGACATGTCATATAAGTAATGCTAGGGTGAGTG1370.13124616799509503No Hit
ATCGGGGGTATGCTGTTCGAATTCATAAGAACAGGGAGGTTAGAAGTAGG1360.13028816676885346No Hit
GTGGTGAGGGTGTTTATTATAATAATGTTTGTGTATTCGGCTATGAAGAA1360.13028816676885346No Hit
GCTAAGCTCGCACTGATTTTTTACCTGAGTAGGCCTAGAAATAAACATGC1350.1293301655426119No Hit
GATTCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCA1350.1293301655426119No Hit
CTTCTAACCTCCCTGTTCTTATGAATTCGAACAGCATACCCCCGATTCCG1320.1264561618638872No Hit
ATACGCCGCAGGCCCCTTCGCCCTATTCTTCATAGCCGAATACACAAACA1310.1254981606376456No Hit
ACGGAGAATTTTGGATTCTCAGGGATGGGTTCGATTCTCATAGTCCTAGA1300.12454015941140405No Hit
CCTTTAACCTCTCCACCCTTATCACAACACAAGAACACCTCTGATTACTC1300.12454015941140405No Hit
ATCGAACCCATCCCTGAGAATCCAAAATTCTCCGTGCCACCTATCACACC1300.12454015941140405No Hit
GTGATGAGTGTGCCTGCAAAGATGGTAGAGTAGATGACGGGTTGGGCCAG1280.1226241569589209No Hit
GTAGAGTTCAGGGGAGAGTGCGTCATATGTTGTTCCTAGGAAGATTGTAG1260.12070815450643778No Hit
GTTCTTATGAATTCGAACAGCATACCCCCGATTCCGCTACGACCAACTCA1240.11879215205395463No Hit
CCTCAAACCTAAGAAATATGTCTGATAAAAGAGTTACTTTGATAGAGTAA1230.11783415082771305No Hit
GTAGATGACGGGTTGGGCCAGGGGATTAATTAGTACGGGAAGGGTATAAC1230.11783415082771305No Hit
ATACACAAACATTATTATAATAAACACCCTCACCACTACAATCTTCCTAG1230.11783415082771305No Hit
GTGGAACGAGGGTTTATTTTTTTGGTTAGAACTGGAATAAAAGCTAGCAT1220.1168761496014715No Hit
ATAGGGCGAAGGGGCCTGCGGCGTATTCGATGTTGAAGCCTGAGACTAGT1220.1168761496014715No Hit
GTACTAATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGC1220.1168761496014715No Hit
GTTTAAGCTCCTATTATTTACTCTATCAAAGTAACTCTTTTATCAGACAT1220.1168761496014715No Hit
GTGTGCCTGCAAAGATGGTAGAGTAGATGACGGGTTGGGCCAGGGGATTA1210.11591814837522993No Hit
CTTCGGCAAGGTCGAAGGGGGTTCGGTTGGTCTCTGCTAGTGTGGAGATA1190.11400214592274678No Hit
ATTAGTACGGGAAGGGTATAACCAACATTTTCGGGGTATGGGCCCGATAG1180.1130441446965052No Hit
AACCCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTA1180.1130441446965052No Hit
CCTTCGACCTTGCCGAAGGGGAGTCCGAACTAGTCTCAGGCTTCAACATC1170.11208614347026365No Hit
CTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGCTCGCACTGAT1160.11112814224402208No Hit
GCTTCTGTGGAACGAGGGTTTATTTTTTTGGTTAGAACTGGAATAAAAGC1150.1101701410177805No Hit
ACTACAATCTTCCTAGGAACAACATATGACGCACTCTCCCCTGAACTCTA1150.1101701410177805No Hit
TTCAACATCGAATACGCCGCAGGCCCCTTCGCCCTATTCTTCATAGCCGA1150.1101701410177805No Hit
CTGAGAATCCAAAATTCTCCGTGCCACCTATCACACCCCATCCTAAAGTA1140.10921213979153893No Hit
CTGAACTCTACACAACATATTTTGTCACCAAGACCCTACTTCTAACCTCC1130.10825413856529735No Hit
ATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGCTCGCA1130.10825413856529735No Hit
GTGAGGAAATACTTGATGGCAGCTTCTGTGGAACGAGGGTTTATTTTTTT1130.10825413856529735No Hit
GTTGGGCCAGGGGATTAATTAGTACGGGAAGGGTATAACCAACATTTTCG1120.1072961373390558No Hit
CCTGAACTCTACACAACATATTTTGTCACCAAGACCCTACTTCTAACCTC1110.10633813611281423No Hit
ACTGATTTTTTACCTGAGTAGGCCTAGAAATAAACATGCTAGCTTTTATT1110.10633813611281423No Hit
CTTTGCAGGCACACTCATCACAGCGCTAAGCTCGCACTGATTTTTTACCT1110.10633813611281423No Hit
AGGATTATGGATGCGGTTGCTTGCGTGAGGAAATACTTGATGGCAGCTTC1110.10633813611281423No Hit
CTTATTTCTAGGACTATGAGAATCGAACCCATCCCTGAGAATCCAAAATT1090.1044221336603311No Hit
CTTGCCGAAGGGGAGTCCGAACTAGTCTCAGGCTTCAACATCGAATACGC1080.1034641324340895No Hit
ACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGCTCGCACTGA1080.1034641324340895No Hit
CCTTGCCGAAGGGGAGTCCGAACTAGTCTCAGGCTTCAACATCGAATACG1070.10250613120784795No Hit
ATGTCATATAAGTAATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGA1060.10154812998160638No Hit
GTAGCGGAATCGGGGGTATGCTGTTCGAATTCATAAGAACAGGGAGGTTA1060.10154812998160638No Hit
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGG1050.1005901287553648No Hit
GTCTTGGTGACAAAATATGTTGTGTAGAGTTCAGGGGAGAGTGCGTCATA1050.1005901287553648No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)