FastQCFastQC Report
Tue 23 Aug 2022
EGAF00006144248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006144248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1117009
Sequences flagged as poor quality0
Sequence length32-251
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT293202.624866943775744TruSeq Adapter, Index 6 (97% over 36bp)
CCGCACAAGAGTGCTACTCTCCTCGCTCCGGGCCCATAACACTTGGGGGT182601.6347227282859853No Hit
GATATTGATTTCACGGAGGATGGTGTTAGGGTTCTTTGTTTTTGGGGTTT52470.46973659120024996No Hit
GATATTGATTTCACGGAGGATGGTGGTCAAGGGACCCCTATCTGAGGGGG51720.4630222316919559No Hit
GCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAG19160.17152950423855134No Hit
ATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGG18870.16893328522867765No Hit
ATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCA18560.16615801663191612No Hit
GACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTG18260.16347227282859852No Hit
ATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTC17970.16087605381872483No Hit
ATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTG17170.1537140703432112No Hit
ATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTA16440.14717876042180503No Hit
ATTTTCGTCTGGGGGGCATGCACGCGATAGCATTGCGAGACGCTGGAGCC15550.1392110538052961No Hit
ATCGCGTGCATGCCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCC15360.13751008272986162No Hit
ATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGC14910.1334814670248852No Hit
ATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATC13720.1228280166050587No Hit
ATCTGGTTCCTACTTCAGGGTCATAAAGCCTAAATAGCCCACACGTTCCC13230.1184413017263066No Hit
ATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGCTTTATGA12840.1149498347819937No Hit
GTGCATGCCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAG12630.11306981411967137No Hit
GTGCTACTCTCCTCGCTCCGGGCCCATAACACTTGGGGGTAGCTAAAGTG11910.10662402899170911No Hit
CTTCAGGGTCATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGAC11690.10465448353594287No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11320.10134206617851779No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)