FastQCFastQC Report
Tue 23 Aug 2022
EGAF00006144249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006144249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1117009
Sequences flagged as poor quality0
Sequence length32-251
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCACAAGAGTGCTACTCTCCTCGCTCCGGGCCCATAACACTTGGGGGT159081.4241604141058846No Hit
GATATTGATTTCACGGAGGATGGTGGTCAAGGGACCCCTATCTGAGGGGG52980.47430235566588985No Hit
GATATTGATTTCACGGAGGATGGTGTTAGGGTTCTTTGTTTTTGGGGTTT28180.25228086792496746No Hit
GCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAG16870.1510283265398936No Hit
ATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGG16440.14717876042180503No Hit
GACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTG15910.14243394636927725No Hit
ATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCA15650.1401063017397353No Hit
ATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTC15030.13455576454621226No Hit
ATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTA14540.13016904966746015No Hit
ATTTTCGTCTGGGGGGCATGCACGCGATAGCATTGCGAGACGCTGGAGCC13460.12050037197551676No Hit
ATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTG13440.12032132238862892No Hit
ATCGCGTGCATGCCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCC12450.1114583678376808No Hit
ATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATC12310.11020502072946592No Hit
ATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGC11780.10546020667693815No Hit
ATCTGGTTCCTACTTCAGGGTCATAAAGCCTAAATAGCCCACACGTTCCC11330.10143159097196174No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)