FastQCFastQC Report
Tue 23 Aug 2022
EGAF00006144250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006144250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55641
Sequences flagged as poor quality0
Sequence length36-251
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCC701112.600420553189196No Hit
GCGATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTT40277.237468773027084No Hit
GGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTA9581.721751945507809No Hit
CGATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTG8501.5276504735716467No Hit
GTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTG8281.48811128484391No Hit
ATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCT8071.450369331967434No Hit
CTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTA7181.2904153412052264No Hit
GTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAAC6751.2131341996010137No Hit
ATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTT6011.0801387466077172No Hit
ACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAA5510.9902769540446792No Hit
CTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTA5250.9435488219118995No Hit
ATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTA5090.9147930482917273No Hit
GTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCC4990.8968206897791197No Hit
ATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGA4270.767419708488345No Hit
ATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCC4100.7368666990169119No Hit
GTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGC4050.7278805197606082No Hit
ACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATT3970.7135026329505221No Hit
ATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGT3780.6793551517765677No Hit
GTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAAC3730.6703689725202638No Hit
CTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCC3440.6182491328337018No Hit
AAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTA3330.5984795384698334No Hit
GTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCG3240.5823044158084866No Hit
GATTACCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGG3010.540967991229489No Hit
GTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAACCCTGT2900.5211983968656206No Hit
ATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGA2780.4996315666504916No Hit
ACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGT2750.49423985909670926No Hit
CCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATC2680.48165920813788393No Hit
GAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCT2600.4672813213277978No Hit
GTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGA2500.4493089628151902No Hit
GTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGGG2420.43493107600510417No Hit
ACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGC2380.4277421326000611No Hit
GTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAA2300.413364245789975No Hit
ATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAA2280.4097697740874535No Hit
ACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAAT2220.39898635897988893No Hit
CTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTAA2210.3971891231286282No Hit
GTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTAT2120.38101400046728134No Hit
CTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTA2110.37921676461602055No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTAC2050.36843334950845597No Hit
ACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACC2000.3594471702521522No Hit
CTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAA1990.35764993440089143No Hit
TGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTA1990.35764993440089143No Hit
TCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAG1990.35764993440089143No Hit
AATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTG1980.3558526985496307No Hit
CTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCAC1970.3540554626983699No Hit
CCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAAC1950.3504609909958484No Hit
GAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTAC1900.3414748117395446No Hit
ATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAA1890.33967757588828384No Hit
GTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTTACAGTC1880.33788034003702305No Hit
GGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACT1810.32529968907819773No Hit
CTCCCTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCT1770.31811074567315467No Hit
ACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGC1770.31811074567315467No Hit
GGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGC1740.3127190381193724No Hit
GAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCAT1640.2947466796067648No Hit
GAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGA1640.2947466796067648No Hit
TGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGC1620.2911522079042433No Hit
CCCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAG1610.2893549720529825No Hit
GAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAA1600.28755773620172176No Hit
GAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGG1580.28396326449920023No Hit
GTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTTAC1550.2785715569454179No Hit
CTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGA1520.2731798493916357No Hit
ATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAG1510.2713826135403749No Hit
AGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTA1480.26599090598659264No Hit
AAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAAT1460.2623964342840711No Hit
AATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGG1450.2605991984328103No Hit
GGTAATCGCATAAAACTTAAAACTTTACAGTCAGAGGTTCAATTCCTCTT1420.25520749087902805No Hit
AGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCG1370.24622131162272426No Hit
CTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCT1350.24262683992020273No Hit
TTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCG1340.24082960406894197No Hit
ATCGCATAAAACTTAAAACTTTACAGTCAGAGGTTCAATTCCTCTTCTTA1330.23903236821768123No Hit
ATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTT1330.23903236821768123No Hit
CATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTT1320.23723513236642044No Hit
ACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCAT1290.23184342481263817No Hit
CCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGA1280.23004618896137738No Hit
AATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCA1260.22645171725885588No Hit
AACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGA1250.2246544814075951No Hit
ATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTTACAGTCAGAGGT1220.21926277385381282No Hit
ATTACCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGG1220.21926277385381282No Hit
CAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATAT1220.21926277385381282No Hit
AAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATC1190.21387106630003055No Hit
CCCTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTC1180.21207383044876982No Hit
AAATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAG1150.2066821228949875No Hit
ATAAAACTTAAAACTTTACAGTCAGAGGTTCAATTCCTCTTCTTAACAAC1100.19769594363868373No Hit
AGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGG1100.19769594363868373No Hit
CAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCC1100.19769594363868373No Hit
CTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGA1090.19589870778742294No Hit
GATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGT1080.19410147193616217No Hit
GCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCC1060.19050700023364067No Hit
CGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAA1060.19050700023364067No Hit
TGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCC1060.19050700023364067No Hit
GCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGT1050.1887097643823799No Hit
TGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAA1040.18691252853111914No Hit
CACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATAC1030.18511529267985838No Hit
GACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAAC1030.18511529267985838No Hit
CCTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCC1000.1797235851260761No Hit
TCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACCC990.17792634927481535No Hit
GAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCT980.17612911342355458No Hit
AGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAA970.17433187757229382No Hit
TGCGATTACCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTG960.17253464172103306No Hit
CCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAAT960.17253464172103306No Hit
TTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTAT950.1707374058697723No Hit
ATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGG920.16534569831599No Hit
TACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTAT920.16534569831599No Hit
ACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAA900.16175122661346847No Hit
GATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTT880.15815675491094697No Hit
TTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTTACAG880.15815675491094697No Hit
GTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTT880.15815675491094697No Hit
TCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATA870.1563595190596862No Hit
ATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAA870.1563595190596862No Hit
CAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATT860.15456228320842544No Hit
ACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGAT850.15276504735716467No Hit
TCTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTA840.1509678115059039No Hit
GGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCT830.14917057565464317No Hit
CCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATA830.14917057565464317No Hit
AACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGG830.14917057565464317No Hit
GGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGA830.14917057565464317No Hit
GGGCTCTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATAAT810.14557610395212164No Hit
ACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCAT810.14557610395212164No Hit
TTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACC800.14377886810086088No Hit
TTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTA800.14377886810086088No Hit
AAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAACCCT790.14198163224960011No Hit
TAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGAT780.14018439639833935No Hit
AGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCA780.14018439639833935No Hit
GAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAAC770.13838716054707859No Hit
GAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAA770.13838716054707859No Hit
GAGCCCGGTAATCGCATAAAACTTAAAACTTTACAGTCAGAGGTTCAATT740.13299545299329632No Hit
GGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTT730.13119821714203556No Hit
TAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAA730.13119821714203556No Hit
AGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGAC720.1294009812907748No Hit
AGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATG720.1294009812907748No Hit
GAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTG720.1294009812907748No Hit
AAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAACCC720.1294009812907748No Hit
ACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACA690.1240092737369925No Hit
TATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTG690.1240092737369925No Hit
TGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGT680.12221203788573175No Hit
GGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTTACAGTCAGAGGTTC680.12221203788573175No Hit
AAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCAC670.12041480203447098No Hit
TCTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAG670.12041480203447098No Hit
AGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTT660.11861756618321022No Hit
CCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATC660.11861756618321022No Hit
GGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTA650.11682033033194945No Hit
GCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTT640.11502309448068869No Hit
TATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGG640.11502309448068869No Hit
GCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCACAC640.11502309448068869No Hit
GATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTTACAGTCAGAGG630.11322585862942794No Hit
TTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAACCCTGTTC630.11322585862942794No Hit
AAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATT630.11322585862942794No Hit
CGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTA620.11142862277816717No Hit
CCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATA610.10963138692690641No Hit
AGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTT610.10963138692690641No Hit
CACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCA610.10963138692690641No Hit
TCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTT600.10783415107564565No Hit
AAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCA600.10783415107564565No Hit
CTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCA590.10603691522438491No Hit
ATGCGATTACCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGGGTGGGT590.10603691522438491No Hit
ATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCCCT590.10603691522438491No Hit
CTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTT570.10244244352186338No Hit
GTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTT570.10244244352186338No Hit
AAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACT570.10244244352186338No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)