FastQCFastQC Report
Tue 23 Aug 2022
EGAF00006144256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006144256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77326
Sequences flagged as poor quality0
Sequence length32-251
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT1702822.021053720611437TruSeq Adapter, Index 2 (97% over 37bp)
CCTCCCTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCC855411.062255903577062No Hit
GCGATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTT52156.744174016501566No Hit
GGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTA10441.3501280293820965No Hit
CGATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTG9311.2039934821405478No Hit
ATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCT8961.1587305692781211No Hit
GTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTG8731.128986369397098No Hit
CTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTA8021.0371673175904612No Hit
GTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAAC6700.8664614747950238No Hit
ATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTT6450.8341308227504332No Hit
ACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAA6040.781108553397305No Hit
ATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTA6010.777228875151954No Hit
CTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTA5610.7254998318806094No Hit
GTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCC4330.5599668934123063No Hit
ATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGA4240.5483278586762538No Hit
ATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCC4200.5431549543491193No Hit
ACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATT4140.5353955978584176No Hit
GTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGC4130.534102371776634No Hit
CTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCC3910.5056513979773943No Hit
GTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAAC3840.496598815404909No Hit
AAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTA3590.4642681633603186No Hit
GATTACCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGG3570.4616817111967514No Hit
ATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGT3420.4422833199699971No Hit
GTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAACCCTGT3370.43581718956107907No Hit
CCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATC3370.43581718956107907No Hit
GTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCG3020.3905542766986525No Hit
ACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGC2970.38408814628973437No Hit
ATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGA2890.37374233763546544No Hit
GTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGGG2860.3698626593901146No Hit
ACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACC2740.3543439464087112No Hit
GAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCT2700.34917104208157673No Hit
ACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGT2650.3427049116726586No Hit
CTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTA2580.3336523291001733No Hit
AATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTG2550.32977265085482244No Hit
CTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAA2450.3168403900369863No Hit
CTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTAA2420.3129607117916354No Hit
CTCCCTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCT2410.3116674857098518No Hit
GTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAA2320.30002845097379927No Hit
ATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAA2200.2845097379923958No Hit
GTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGA2100.27157747717455966No Hit
ACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAAT2080.2689910250109924No Hit
TCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAG2060.2664045728474252No Hit
GTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTAT2030.26252489460207434No Hit
TGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTA2000.2586452163567235No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTAC1990.2573519902749398No Hit
CCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAAC1970.25476553811137265No Hit
CTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCT1960.253472312029589No Hit
AGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCG1950.2521790859478054No Hit
CTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCAC1850.2392468251299692No Hit
GGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACT1850.2392468251299692No Hit
GAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGA1840.2379535990481856No Hit
ATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAA1830.236660372966402No Hit
GAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCAT1780.23019424255748389No Hit
GGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGC1780.23019424255748389No Hit
ACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGC1750.22631456431213304No Hit
ATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAG1720.2224348860667822No Hit
GAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGG1660.2146755295760805No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1640.2120890774125133No Hit
CTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGA1640.2120890774125133No Hit
GAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTAC1640.2120890774125133No Hit
TTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCG1630.21079585133072964No Hit
AATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGG1590.20562294700359518No Hit
AAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAAT1580.20432972092181154No Hit
TGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGC1580.20432972092181154No Hit
CATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTT1510.19527713834932625No Hit
TGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCC1510.19527713834932625No Hit
GTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTTACAGTC1510.19527713834932625No Hit
GAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAA1480.19139746010397538No Hit
GTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTTAC1480.19139746010397538No Hit
CCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGA1480.19139746010397538No Hit
AAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATC1440.1862245557768409No Hit
GGTAATCGCATAAAACTTAAAACTTTACAGTCAGAGGTTCAATTCCTCTT1420.18363810361327368No Hit
ATTACCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGG1390.17975842536792283No Hit
ACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCAT1370.1771719732043556No Hit
AGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGG1370.1771719732043556No Hit
CAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATAT1360.17587874712257195No Hit
CCCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAG1330.1719990688772211No Hit
AGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTA1320.17070584279543752No Hit
CAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCC1300.16811939063187026No Hit
TATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT1280.16553293846830303TruSeq Adapter, Index 2 (97% over 35bp)
ATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTT1260.1629464863047358No Hit
GCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCC1250.16165326022295218No Hit
TACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTAT1220.15777358197760133No Hit
ATAAAACTTAAAACTTTACAGTCAGAGGTTCAATTCCTCTTCTTAACAAC1210.1564803558958177No Hit
AATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCA1190.15389390373225048No Hit
TGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAA1180.15260067765046686No Hit
GTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTT1150.148720999405116No Hit
TTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTTACAG1140.14742777332333237No Hit
AAATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAG1140.14742777332333237No Hit
GAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCT1120.14484132115976514No Hit
AGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAA1090.1409616429144143No Hit
GATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGT1080.13966841683263068No Hit
TGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGT1080.13966841683263068No Hit
CCCTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTC1040.1344955125054962No Hit
TCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATA1040.1344955125054962No Hit
TCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACCC1030.1332022864237126No Hit
AACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGA1030.1332022864237126No Hit
GATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTT1020.13190906034192898No Hit
CGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAA1000.12932260817836175No Hit
TATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGG1000.12932260817836175No Hit
TGCGATTACCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTG990.12802938209657813No Hit
CCTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCC980.1267361560147945No Hit
ATCGCATAAAACTTAAAACTTTACAGTCAGAGGTTCAATTCCTCTTCTTA970.1254429299330109No Hit
ATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTTACAGTCAGAGGT960.12414970385122726No Hit
AAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAACCCT960.12414970385122726No Hit
CACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATAC960.12414970385122726No Hit
TTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTAT950.12285647776944365No Hit
CCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATA950.12285647776944365No Hit
ACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCAT940.12156325168766005No Hit
TAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGAT910.11768357344230918No Hit
CTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGA910.11768357344230918No Hit
CCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACCCA900.11639034736052557No Hit
AAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCAC900.11639034736052557No Hit
GACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAAC900.11639034736052557No Hit
GGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGA900.11639034736052557No Hit
GGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCT880.11380389519695833No Hit
CAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATT870.11251066911517472No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCTCATTATCTCGTAT870.11251066911517472TruSeq Adapter, Index 11 (97% over 37bp)
TTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACC870.11251066911517472No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTACCTCGTAT850.10992421695160748TruSeq Adapter, Index 2 (97% over 37bp)
AAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAACCC840.10863099086982385No Hit
ATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAA830.10733776478804025No Hit
TCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTT820.10604453870625664No Hit
GAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAAC810.10475131262447301No Hit
TAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAA810.10475131262447301No Hit
ACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAA800.1034580865426894No Hit
TCTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTA800.1034580865426894No Hit
AACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGG800.1034580865426894No Hit
ATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGG790.10216486046090577No Hit
ACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGAT790.10216486046090577No Hit
CACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCA780.10087163437912217No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)