FastQCFastQC Report
Tue 23 Aug 2022
EGAF00006144257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006144257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77326
Sequences flagged as poor quality0
Sequence length32-251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCC799410.338049297778237No Hit
GCGATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTT55397.163179266999458No Hit
CGATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTG10861.4044435248170084No Hit
ATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCT9931.284173499211132No Hit
GGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTA9291.2014070299769806No Hit
GTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTG8371.0824302304528877No Hit
CTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTA7070.9143108398210176No Hit
ATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTT6230.8056798489511937No Hit
GTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAAC6070.7849882316426557No Hit
ACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAA5570.7203269275534748No Hit
ATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTA5520.7138607971445567No Hit
CTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTA5130.6634249799549957No Hit
ATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGA4500.5819517368026278No Hit
GTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAAC4330.5599668934123063No Hit
GTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCC4220.5457414065126865No Hit
CTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCC3920.506944624059178No Hit
ACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATT3800.4914259110777746No Hit
CTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGA3770.4875462328324237No Hit
GTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGC3760.4862530067506402No Hit
ATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCC3720.4810801024235057No Hit
GATTACCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGG3520.4552155807878333No Hit
ATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGT3420.4422833199699971No Hit
AAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTA3350.4332307373975119No Hit
GTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCG3300.42676460698859375No Hit
CCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATC3200.4138323461707576No Hit
ATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGA3100.4009000853529214No Hit
CTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAA3080.39831363318935414No Hit
ACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGC3070.3970204071075706No Hit
GTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAACCCTGT3060.39572718102578697No Hit
CTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTA3050.39443395494400335No Hit
CTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTAA2970.38408814628973437No Hit
AATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTG2720.3517574942451439No Hit
GAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCT2540.3284794247730388No Hit
GTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGGG2490.32201329436412074No Hit
GTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAA2310.2987352248920156No Hit
GTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTAT2300.297441998810232No Hit
ACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAAT2280.29485554664666475No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTAC2220.2870961901559631No Hit
ACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGT2200.2845097379923958No Hit
GTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGA2190.28321651191061226No Hit
ACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACC2050.2651113467656416No Hit
CTCCCTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCT2040.26381812068385796No Hit
ATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAA1970.25476553811137265No Hit
TGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGC1960.253472312029589No Hit
GGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGC1900.2457129555388873No Hit
TCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAG1900.2457129555388873No Hit
GAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGA1880.2431265033753201No Hit
CTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCT1850.2392468251299692No Hit
CTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCAC1830.236660372966402No Hit
GAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTAC1800.23278069472105115No Hit
GAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGG1760.22760779039391665No Hit
ATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAA1730.2237281121485658No Hit
GGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACT1710.22114165998499855No Hit
CCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAAC1650.21338230349429688No Hit
ACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGC1650.21338230349429688No Hit
TGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTA1630.21079585133072964No Hit
AATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGG1630.21079585133072964No Hit
GAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCAT1630.21079585133072964No Hit
CATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTT1620.20950262524894603No Hit
AAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAAT1590.20562294700359518No Hit
AGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCG1570.20303649484002795No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1540.19915681659467707No Hit
GTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTTACAGTC1530.19786359051289348No Hit
ATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAG1520.19657036443110984No Hit
TTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCG1520.19657036443110984No Hit
CCCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAG1440.1862245557768409No Hit
CTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGA1430.1849313296950573No Hit
GAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAA1380.1784651992861392No Hit
AGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTA1330.1719990688772211No Hit
ATTACCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGG1330.1719990688772211No Hit
TGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAA1310.16941261671365387No Hit
GATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTT1290.16682616455008664No Hit
GTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTTAC1290.16682616455008664No Hit
TCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATA1270.1642397123865194No Hit
CACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATAC1230.15906680805938495No Hit
AAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATC1210.1564803558958177No Hit
CAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATAT1210.1564803558958177No Hit
GAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCT1160.15001422548689963No Hit
CCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGA1160.15001422548689963No Hit
CCCTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTC1140.14742777332333237No Hit
CAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCC1140.14742777332333237No Hit
ACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGAT1120.14484132115976514No Hit
ACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCAT1120.14484132115976514No Hit
GATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGT1110.14354809507798155No Hit
CAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATT1100.1422548689961979No Hit
AATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCA1100.1422548689961979No Hit
TATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGG1070.13837519075084706No Hit
TGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCC1070.13837519075084706No Hit
TTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTTACAG1060.13708196466906344No Hit
GACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAAC1060.13708196466906344No Hit
AGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGG1060.13708196466906344No Hit
TAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGAT1050.13578873858727983No Hit
TTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTAT1030.1332022864237126No Hit
AAATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAG1020.13190906034192898No Hit
ATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAA990.12802938209657813No Hit
ACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCAT990.12802938209657813No Hit
TAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAA990.12802938209657813No Hit
GGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCT980.1267361560147945No Hit
ATCGCATAAAACTTAAAACTTTACAGTCAGAGGTTCAATTCCTCTTCTTA980.1267361560147945No Hit
AGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAA970.1254429299330109No Hit
GAGCCCGGTAATCGCATAAAACTTAAAACTTTACAGTCAGAGGTTCAATT970.1254429299330109No Hit
TCTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTA960.12414970385122726No Hit
AACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGA950.12285647776944365No Hit
TCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACCC930.12027002560587642No Hit
ATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGG910.11768357344230918No Hit
AAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAACCC900.11639034736052557No Hit
ATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTT890.11509712127874194No Hit
GCGATTACCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGT880.11380389519695833No Hit
TTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTA870.11251066911517472No Hit
GGTAATCGCATAAAACTTAAAACTTTACAGTCAGAGGTTCAATTCCTCTT870.11251066911517472No Hit
AAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCAC860.1112174430333911No Hit
TACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTAT860.1112174430333911No Hit
GGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGA860.1112174430333911No Hit
AAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATT850.10992421695160748No Hit
ATAAAACTTAAAACTTTACAGTCAGAGGTTCAATTCCTCTTCTTAACAAC840.10863099086982385No Hit
ACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAA840.10863099086982385No Hit
TGCGATTACCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTG820.10604453870625664No Hit
ACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAAAC820.10604453870625664No Hit
ATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTTACAGTCAGAGGT810.10475131262447301No Hit
GAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAAC800.1034580865426894No Hit
CCTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCC790.10216486046090577No Hit
GTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTT790.10216486046090577No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)