FastQCFastQC Report
Tue 23 Aug 2022
EGAF00006144258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006144258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50737
Sequences flagged as poor quality0
Sequence length32-251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCC1074121.16995486528569No Hit
GCGATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTT730314.39383487395786No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT592511.677868222401798TruSeq Adapter, Index 27 (97% over 39bp)
CGATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTG5691.1214695389952105No Hit
GGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTA5621.1076729014328794No Hit
GTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTG5331.050515402960364No Hit
ATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCT4840.9539389400240456No Hit
ATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTT4350.8573624770877268No Hit
CTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTA4250.8376529948558251No Hit
CTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTA3840.7568441177050278No Hit
GTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAAC3650.7193961014644146No Hit
ATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTA3110.612964897412145No Hit
ATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGA2840.5597492953860101No Hit
ACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAA2360.4651437806728817No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2320.457259987780121No Hit
ACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATT2210.4355795573250291No Hit
ATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGT2200.4336086091018389No Hit
GTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCG2180.4296667126554585No Hit
GTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCC2140.4217829197626978No Hit
CTCCCTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCT2100.4138991268699372No Hit
GATTACCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGG2080.40995723042355675No Hit
ATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGA1900.3744801624061336No Hit
CTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCC1680.3311193014959497No Hit
GTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAAC1680.3311193014959497No Hit
GTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGA1600.3153517157104283No Hit
CCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATC1570.30943887104085777No Hit
ACTCCCTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCC1560.30746792281766755No Hit
GTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAACCCTGT1550.3054969745944774No Hit
AATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTG1510.2976131817017167No Hit
GAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCT1500.29564223347852653No Hit
ATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCC1490.29367128525533637No Hit
GTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGC1380.2719908548002444No Hit
GTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTAT1370.2700199065770542No Hit
ATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAA1370.2700199065770542No Hit
ACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAAT1360.26804895835386405No Hit
GAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGA1350.2660780101306739No Hit
GTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAA1350.2660780101306739No Hit
GATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGT1340.26410706190748373No Hit
GTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGGG1300.256223269014723No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTAC1230.24242663145239174No Hit
AAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTA1220.24045568322920158No Hit
CTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTA1190.234542838559631No Hit
GCTCCCTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCC1180.23257189033644085No Hit
CTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCAC1160.2286299938900605No Hit
TGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTA1160.2286299938900605No Hit
ACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGT1120.2207462009972998No Hit
ATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAA1100.21680430455091945No Hit
GGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGC1050.2069495634349686No Hit
CTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTAA1030.20300766698858821No Hit
TGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGC1020.20103671876539803No Hit
CTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAA990.19512387409582752No Hit
GGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACT980.19315292587263733No Hit
ACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGC970.19118197764944717No Hit
TCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAG970.19118197764944717No Hit
GAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGG920.18132723653349628No Hit
ATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAG910.1793562883103061No Hit
ATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTT900.1773853400871159No Hit
CCCTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTC900.1773853400871159No Hit
ACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACC900.1773853400871159No Hit
ATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCCCT890.17541439186392574No Hit
ACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGC880.17344344364073555No Hit
GAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCAT840.16555965074797485No Hit
GAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTAC830.16358870252478466No Hit
AAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAAT790.15570490963202396No Hit
ATCGCATAAAACTTAAAACTTTACAGTCAGAGGTTCAATTCCTCTTCTTA790.15570490963202396No Hit
TCTCCCTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCC740.14585016851607308No Hit
GTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTTAC740.14585016851607308No Hit
ATTACCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGG720.14190827206969273No Hit
CTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCT710.13993732384650254No Hit
GGTAATCGCATAAAACTTAAAACTTTACAGTCAGAGGTTCAATTCCTCTT700.13796637562331238No Hit
ACGATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTT700.13796637562331238No Hit
CAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATT680.13402447917693203No Hit
AATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCA650.1281116345073615No Hit
CATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTT650.1281116345073615No Hit
GTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTTACAGTC650.1281116345073615No Hit
ATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTTACAGTCAGAGGT640.1261406862841713No Hit
TCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACCC630.12416973806098114No Hit
AGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTA630.12416973806098114No Hit
CAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATAT620.12219878983779095No Hit
TTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTAT620.12219878983779095No Hit
TTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCG610.12022784161460079No Hit
TCCCTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTT600.11825689339141061No Hit
AAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATC590.11628594516822043No Hit
GTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTT590.11628594516822043No Hit
ATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAA580.11431499694503025No Hit
CCCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAG580.11431499694503025No Hit
GGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCT580.11431499694503025No Hit
CCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAAC570.11234404872184006No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT570.11234404872184006TruSeq Adapter, Index 27 (97% over 39bp)
TACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTAT560.1103731004986499No Hit
AATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGG560.1103731004986499No Hit
CTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGA540.10643120405226954No Hit
ATAAAACTTAAAACTTTACAGTCAGAGGTTCAATTCCTCTTCTTAACAAC530.10446025582907938No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT520.10248930760588919TruSeq Adapter, Index 27 (97% over 36bp)
TGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCC520.10248930760588919No Hit
CCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGA520.10248930760588919No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTAGATCTCGTAT510.10051835938269901TruSeq Adapter, Index 27 (97% over 39bp)
GCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCC510.10051835938269901No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)