FastQCFastQC Report
Tue 23 Aug 2022
EGAF00006144260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006144260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences937465
Sequences flagged as poor quality0
Sequence length32-251
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT481525.136405092456784TruSeq Adapter, Index 6 (97% over 36bp)
CCGCACAAGAGTGCTACTCTCCTCGCTCCGGGCCCATAACACTTGGGGGT115241.2292725595088883No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN36430.3886011744438459No Hit
GATATTGATTTCACGGAGGATGGTGTTAGGGTTCTTTGTTTTTGGGGTTT35290.3764407204535636No Hit
GATATTGATTTCACGGAGGATGGTGGTCAAGGGACCCCTATCTGAGGGGG17200.1834735163446102No Hit
ATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCA17120.18262015115230967No Hit
GACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTG16220.17301979273892892No Hit
GCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAG14300.1525390281237166No Hit
ATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGG14270.1522190161766039No Hit
ATCGCGTGCATACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCC13720.1463521304795379No Hit
ATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTC13570.14475207074397445No Hit
ATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATC12490.13323164064791754No Hit
ATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTG12250.130671545071016No Hit
ATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTA11060.11797773783554587No Hit
GTCTCGCAATGCTATCGCGTGCATACCCCCCAGACGAAAATACCAAATGC10540.11243086408559252No Hit
ATCTGGTTCCTACTTCAGGGTCATAAAGCCTAAATAGCCCACACGTTCCC9770.1042172241097001No Hit
GTGCATACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAG9700.10347052956643714No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)