FastQCFastQC Report
Tue 23 Aug 2022
EGAF00006144261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006144261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences937465
Sequences flagged as poor quality0
Sequence length32-251
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCACAAGAGTGCTACTCTCCTCGCTCCGGGCCCATAACACTTGGGGGT124611.3292229576570858No Hit
GATATTGATTTCACGGAGGATGGTGTTAGGGTTCTTTGTTTTTGGGGTTT36580.3902012341794094No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35570.3794274986266154No Hit
GATATTGATTTCACGGAGGATGGTGGTCAAGGGACCCCTATCTGAGGGGG20130.21472801651261647No Hit
ATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGG15610.1665128831476375No Hit
GCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAG15220.16235272783517252No Hit
GACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTG14100.15040561514296535No Hit
ATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCA14000.1493389086525897No Hit
ATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTC13660.14571210658531253No Hit
ATCGCGTGCATACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCC11940.1273647549508515No Hit
ATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTA11570.12341794093646163No Hit
ATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTG11190.11936445627303419No Hit
ATCTGGTTCCTACTTCAGGGTCATAAAGCCTAAATAGCCCACACGTTCCC10260.10944408591254075No Hit
ATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATC10010.10677731968660163No Hit
CTTCAGGGTCATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGAC9500.10133711658568588No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)