FastQCFastQC Report
Tue 23 Aug 2022
EGAF00006144263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006144263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79907
Sequences flagged as poor quality0
Sequence length32-251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCC923511.557185227827349No Hit
GCGATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTT78789.858961042211572No Hit
GGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTA14141.7695571101405385No Hit
ATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCT14001.7520367427134043No Hit
GTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTG12301.539289423955348No Hit
CTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTA9501.1888820754126672No Hit
ATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTT9381.1738646176179808No Hit
GTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAAC9241.1563442501908467No Hit
ACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAA7980.9986609433466404No Hit
ATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTA7540.9435969314327907No Hit
GTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCC6970.8722640069080306No Hit
CTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTA6620.8284630883401956No Hit
GTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAAC6110.7646388927127785No Hit
ATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGA5870.734603977123406No Hit
GTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGC5390.6745341459446607No Hit
ACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATT5360.670779781495989No Hit
GTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCG5170.6470021399877357No Hit
GATTACCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGG5160.6457506851715119No Hit
ATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGT5010.626978862928154No Hit
AAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTA4950.6194701340308109No Hit
CTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCC4850.6069555858685722No Hit
ATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCC4840.6057041310523483No Hit
GTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAACCCTGT4570.5719148510143041No Hit
ATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGA4460.5581488480358417No Hit
CCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATC4340.5431313902411553No Hit
CTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGA4310.5393770257924838No Hit
CGATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTG4250.5318682968951406No Hit
ACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGC3970.49682756204087253No Hit
CTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTA3970.49682756204087253No Hit
GAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCT3740.4680441012677237No Hit
GTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAA3720.465541191635276No Hit
CTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTAA3680.46053537237038056No Hit
CTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAA3560.44551791457569423No Hit
GTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTAT3530.4417635501270226No Hit
AATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTG3510.4392606404945749No Hit
ACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAAT3480.4355062760459033No Hit
GTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGA3440.43050045678100796No Hit
ACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGT3430.4292490019647841No Hit
GTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGGG3250.4067228152727546No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTAC3170.3967111767429637No Hit
GTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTTACAGTC3000.3754364448671581No Hit
ATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAA2850.35666462262380016No Hit
GGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACT2780.34790443891023315No Hit
GAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGA2710.33914425519666613No Hit
TCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAG2670.33413843593177067No Hit
TGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTA2600.32537825221820366No Hit
CTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCAC2580.32287534258575595No Hit
GAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGG2530.31661806850463664No Hit
ATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAG2480.3103607944235173No Hit
CTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCT2450.3066064299748458No Hit
GGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGC2410.3016006107099503No Hit
GTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTTAC2400.30034915589372646No Hit
GAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTAC2400.30034915589372646No Hit
TGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGC2370.29659479144505485No Hit
ACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGC2330.29158897218015944No Hit
ATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAA2300.28783460773148783No Hit
CCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAAC2230.2790744240179209No Hit
ACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACC2140.2678113306719061No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2140.2678113306719061No Hit
GAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCAT2130.26655987585568225No Hit
AAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAAT2060.2577996921421152No Hit
CCCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAG2050.2565482373258913No Hit
CTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGA1990.24903950842854822No Hit
CTCCCTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCT1970.2465365987961005No Hit
TTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCG1970.2465365987961005No Hit
GAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAA1960.24528514397987664No Hit
AGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGG1950.24403368916365276No Hit
GGTAATCGCATAAAACTTAAAACTTTACAGTCAGAGGTTCAATTCCTCTT1950.24403368916365276No Hit
TGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAA1940.2427822343474289No Hit
AAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATC1900.23777641508253344No Hit
CATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTT1900.23777641508253344No Hit
ATAAAACTTAAAACTTTACAGTCAGAGGTTCAATTCCTCTTCTTAACAAC1790.22401041210407097No Hit
GAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCT1780.22275895728784711No Hit
ATTACCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGG1720.21525022839050398No Hit
CCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGA1720.21525022839050398No Hit
ATCGCATAAAACTTAAAACTTTACAGTCAGAGGTTCAATTCCTCTTCTTA1690.2114958639418324No Hit
CAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATAT1690.2114958639418324No Hit
AGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTA1670.20899295430938467No Hit
AATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGG1610.2014842254120415No Hit
GACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAAC1600.20023277059581765No Hit
TTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTTACAG1580.19772986096336992No Hit
AGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCG1550.19397549651469834No Hit
CAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATT1530.1914725868822506No Hit
GGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCT1510.1889696772498029No Hit
AAATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAG1500.18771822243357905No Hit
ACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCAT1470.18396385798490747No Hit
TCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACCC1470.18396385798490747No Hit
ATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTT1450.18146094835245974No Hit
AGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAA1450.18146094835245974No Hit
ACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCAT1440.18020949353623586No Hit
TGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCC1420.17770658390378816No Hit
CGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAA1420.17770658390378816No Hit
GCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCC1370.17144930982266887No Hit
TCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATA1360.170197855006445No Hit
GTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTT1340.16769494537399726No Hit
ATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAA1300.16268912610910183No Hit
GGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGA1270.15893476166043025No Hit
TACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTAT1260.1576833068442064No Hit
AATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCA1230.1539289423955348No Hit
AACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGA1230.1539289423955348No Hit
TTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTAT1230.1539289423955348No Hit
TCTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTA1220.15267748757931096No Hit
TTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACC1180.1476716683144155No Hit
TATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGG1170.14642021349819165No Hit
GAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAAC1170.14642021349819165No Hit
TAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGAT1130.1414143942332962No Hit
ACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAA1130.1414143942332962No Hit
TTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTA1110.13891148460084848No Hit
GCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGT1100.13766002978462463No Hit
CACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATAC1090.13640857496840078No Hit
GGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTT1090.13640857496840078No Hit
GATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTT1080.1351571201521769No Hit
TTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTG1080.1351571201521769No Hit
GTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTT1080.1351571201521769No Hit
AGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGAC1040.13015130088728147No Hit
CCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATA1040.13015130088728147No Hit
GAGCCCGGTAATCGCATAAAACTTAAAACTTTACAGTCAGAGGTTCAATT1040.13015130088728147No Hit
CCTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCC1020.12764839125483374No Hit
TCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTT1010.12639693643860989No Hit
CAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCC1010.12639693643860989No Hit
TAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAA1000.125145481622386No Hit
CCCTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTC980.12264257198993832No Hit
GAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTC980.12264257198993832No Hit
ATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTTACAGTCAGAGGT970.12139111717371445No Hit
GGGCTCTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATAAT960.12013966235749059No Hit
CCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAAT950.11888820754126672No Hit
AGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAACCCTG940.11763675272504287No Hit
TCTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAG940.11763675272504287No Hit
AACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGG930.116385297908819No Hit
TGCGATTACCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTG930.116385297908819No Hit
AAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATT910.11388238827637127No Hit
AGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATG910.11388238827637127No Hit
CACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCA900.11263093346014742No Hit
AAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAACCC880.11012802382769969No Hit
CCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACCCA860.10762511419525199No Hit
ACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACA850.10637365937902812No Hit
AAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAACCCT850.10637365937902812No Hit
TATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTT840.10512220456280427No Hit
TACCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTA830.1038707497465804No Hit
CTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTT820.10261929493035654No Hit
ATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGG810.10136784011413268No Hit
TTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAACCCTGTTC810.10136784011413268No Hit
TGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGC810.10136784011413268No Hit
AAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCAC800.10011638529790882No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)