FastQCFastQC Report
Tue 23 Aug 2022
EGAF00006144266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006144266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66873
Sequences flagged as poor quality0
Sequence length32-251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCC62959.413365633364736No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT60148.993166150763388TruSeq Adapter, Index 2 (97% over 37bp)
GCGATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTT36905.517922031313086No Hit
GGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTA11321.692760904999028No Hit
ATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCT10821.6179923137888235No Hit
GTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTG9911.4819134777862515No Hit
CTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTA8881.3278901798932305No Hit
GTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAAC8231.2306910113199647No Hit
ATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTT7751.1589131637581684No Hit
CGATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTG7111.0632093670091067No Hit
ACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAA6991.0452649051186578No Hit
ATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTA6420.9600287111390247No Hit
GTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCC5900.8822693762804121No Hit
CTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTA5600.8374082215542894No Hit
ATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGA5090.761144258519881No Hit
CTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCC5030.7521720275746564No Hit
ACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATT4940.7387136811568196No Hit
ATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCC4740.7088062446727379No Hit
GTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGC4610.6893664109580847No Hit
ATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGT4410.6594589744740029No Hit
GTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAAC3860.577213524142778No Hit
GATTACCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGG3820.5712320368459618No Hit
AAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTA3660.5473060876586964No Hit
GTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCG3570.5338477412408595No Hit
GTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAACCCTGT3550.5308569975924514No Hit
ATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGA3260.48749121469053275No Hit
GAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCT3240.4845004710421246No Hit
ACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGT3220.48150972739371645No Hit
ACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGC3170.47403286827269603No Hit
CTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTA2930.43814394449179794No Hit
CCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATC2880.4306670853707774No Hit
CTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAA2870.42917171354657335No Hit
ACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACC2720.406741136183512No Hit
AATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTG2670.39926427706249157No Hit
ATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAA2650.3962735334140834No Hit
GTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGA2610.3902920461172671No Hit
GTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAA2540.37982444334783844No Hit
ACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAAT2500.3738429560510221No Hit
TCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAG2420.36187998145738937No Hit
GTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTAT2420.36187998145738937No Hit
CTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTAA2390.3573938659847771No Hit
GAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGA2390.3573938659847771No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTAC2380.355898494160573No Hit
GTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGGG2380.355898494160573No Hit
ATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAA2260.337954032270124No Hit
AGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCG2250.3364586604459199No Hit
CCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAAC2240.33496328862171576No Hit
AATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGG2200.3289818013248994No Hit
GGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGC2190.32748642950069534No Hit
CCCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAG2140.3200095703796749No Hit
TGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTA2080.3110373394344504No Hit
GTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTTACAGTC2080.3110373394344504No Hit
TTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCG2070.30954196761024627No Hit
AAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAAT2060.3080465957860422No Hit
GAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCAT2040.30505585213763403No Hit
ACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGC2040.30505585213763403No Hit
CTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCAC2020.30206510848922585No Hit
GAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGG2020.30206510848922585No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2020.30206510848922585No Hit
ATAAAACTTAAAACTTTACAGTCAGAGGTTCAATTCCTCTTCTTAACAAC1960.2930928775440013No Hit
TGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGC1950.29159750571979726No Hit
GAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTAC1910.2856160184229809No Hit
GGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACT1890.28262527477457267No Hit
CCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGA1880.2811299029503686No Hit
GAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAA1850.27664378747775636No Hit
CTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGA1840.27514841565355225No Hit
GTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTTAC1800.2691669283567359No Hit
ATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAG1780.2661761847083277No Hit
AATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCA1710.25570858193889906No Hit
AAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATC1660.24823172281787867No Hit
CATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTT1630.24374560734526637No Hit
CTCCCTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCT1600.23925949187265416No Hit
CTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCT1600.23925949187265416No Hit
GGTAATCGCATAAAACTTAAAACTTTACAGTCAGAGGTTCAATTCCTCTT1530.22879188910322554No Hit
ATTACCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGG1530.22879188910322554No Hit
CAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATAT1500.22430577363061324No Hit
CAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCC1480.2213150299822051No Hit
ATCGCATAAAACTTAAAACTTTACAGTCAGAGGTTCAATTCCTCTTCTTA1390.20785668356436826No Hit
ACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCAT1380.2063613117401642No Hit
AGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGG1350.20187519626755193No Hit
TGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCC1350.20187519626755193No Hit
ATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGG1320.19738908079493964No Hit
AGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTA1320.19738908079493964No Hit
AAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCAC1310.19589370897073557No Hit
ACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGAT1290.1929029653223274No Hit
ACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCAT1290.1929029653223274No Hit
GTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTT1280.1914075934981233No Hit
TGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAA1270.18991222167391922No Hit
GCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCC1260.18841684984971513No Hit
ATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTT1240.18542610620130698No Hit
TCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACCC1230.18393073437710286No Hit
CGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAA1230.18393073437710286No Hit
TTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTAT1230.18393073437710286No Hit
TTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTTACAG1230.18393073437710286No Hit
CCTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCC1220.18243536255289877No Hit
TCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATA1220.18243536255289877No Hit
AAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAACCCT1210.18093999072869468No Hit
GAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCT1210.18093999072869468No Hit
GATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGT1190.1779492470802865No Hit
AGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAA1180.17645387525608242No Hit
ATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTTACAGTCAGAGGT1180.17645387525608242No Hit
TACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTAT1180.17645387525608242No Hit
AACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGA1150.17196775978347018No Hit
ATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAA1130.168977016135062No Hit
CAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATT1080.16150015701404152No Hit
GATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTT1070.16000478518983746No Hit
TGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGT1070.16000478518983746No Hit
GGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTT1060.15850941336563337No Hit
GACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAAC1060.15850941336563337No Hit
CTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGA1050.15701404154142928No Hit
TTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACC1030.1540232978930211No Hit
GGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCT1000.14953718242040884No Hit
AAATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAG990.14804181059620475No Hit
ACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAAAC980.14654643877200066No Hit
TAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAA960.14355569512359248No Hit
GAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAA950.1420603232993884No Hit
TTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTA940.1405649514751843No Hit
TATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGG930.1390695796509802No Hit
ACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAA930.1390695796509802No Hit
AAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAACCC930.1390695796509802No Hit
GGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGA930.1390695796509802No Hit
GCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTT920.13757420782677612No Hit
TCTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAG910.13607883600257203No Hit
AACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGG900.13458346417836795No Hit
AAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCA890.13308809235416386No Hit
CACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATAC870.13009734870575568No Hit
CCCTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTC860.12860197688155162No Hit
AGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAACCCTG850.1271066050573475No Hit
AAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATT840.12561123323314344No Hit
AGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATG830.12411586140893933No Hit
CTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCA820.12262048958473525No Hit
GATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTTACAGTCAGAGG810.12112511776053116No Hit
AGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCA800.11962974593632708No Hit
ACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACA800.11962974593632708No Hit
CATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTTA780.1166390022879189No Hit
CCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAAT780.1166390022879189No Hit
GAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTC770.1151436304637148No Hit
TCTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTA770.1151436304637148No Hit
TCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTT760.11364825863951072No Hit
TTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAACCCTGTTC750.11215288681530662No Hit
CACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCA750.11215288681530662No Hit
CCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACCCA740.11065751499110255No Hit
TACCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTA740.11065751499110255No Hit
GGGCTCTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATAAT740.11065751499110255No Hit
GAGCCCGGTAATCGCATAAAACTTAAAACTTTACAGTCAGAGGTTCAATT730.10916214316689846No Hit
GCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGT710.10617139951849028No Hit
CAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTT700.10467602769428618No Hit
CCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATC690.1031806558700821No Hit
GTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTT680.101685284045878No Hit
TGCGATTACCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTG670.10018991222167392No Hit
TAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGAT670.10018991222167392No Hit
GACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGAT670.10018991222167392No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)