FastQCFastQC Report
Tue 23 Aug 2022
EGAF00006144267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006144267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66873
Sequences flagged as poor quality0
Sequence length32-251
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCC58288.715026991461428No Hit
GCGATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTT40386.038311426136109No Hit
GGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTA10981.6419182629760891No Hit
ATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCT9691.4490152976537616No Hit
GTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTG9261.3847143092129859No Hit
CTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTA8711.3024688588817608No Hit
CGATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTG8401.2561123323314343No Hit
ATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTT7651.1439594455161275No Hit
GTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAAC7291.0901260598447804No Hit
ATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTA6881.028815815052413No Hit
ACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAA5950.8897462354014325No Hit
CTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTA5540.828435990609065No Hit
GTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCC5360.8015192977733914No Hit
GTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAAC5080.7596488866956769No Hit
CTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCC4850.7252553347389828No Hit
ATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGA4830.7222645910905746No Hit
ACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATT4240.6340376534625335No Hit
GTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGC4110.6145978197478803No Hit
AAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTA4080.6101117042752681No Hit
ATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGT4010.5996441015058395No Hit
CTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGA3810.5697366650217577No Hit
ATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCC3780.5652505495491453No Hit
GTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAACCCTGT3750.5607644340765331No Hit
ATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGA3600.5383338567134718No Hit
CCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATC3490.5218847666472268No Hit
GATTACCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGG3450.5159032793504105No Hit
GTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCG3450.5159032793504105No Hit
CTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTA3340.49945418928416546No Hit
AATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTG3260.48749121469053275No Hit
ACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGT3240.4845004710421246No Hit
GTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTAT3080.4605745218548592No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTAC2860.42767634172236924No Hit
ACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGC2830.423190226249757No Hit
ACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAAT2810.4201994826013488No Hit
GTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAA2760.4127226234803284No Hit
GAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCT2730.4082365080077161No Hit
CTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAA2730.4082365080077161No Hit
CTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTAA2660.39776890523828756No Hit
GTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGGG2630.39328278976567527No Hit
TGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTA2510.37533832787522614No Hit
GTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGA2510.37533832787522614No Hit
ATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAA2450.36636609693000166No Hit
GAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGA2390.3573938659847771No Hit
CTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCAC2360.35290775051216483No Hit
ACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACC2330.3484216350395526No Hit
TCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAG2300.34393551956694035No Hit
GAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGG2260.337954032270124No Hit
GGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGC2240.33496328862171576No Hit
AGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCG2180.3259910576764913No Hit
GTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTTACAGTC2160.32300031402808305No Hit
AATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGG2090.31253271125865445No Hit
ACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGC2090.31253271125865445No Hit
TGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGC2050.3065512239618381No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2000.2990743648408177No Hit
GAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTAC1990.2975789930166136No Hit
ATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAA1960.2930928775440013No Hit
CTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCT1850.27664378747775636No Hit
CATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTT1830.2736530438293482No Hit
GAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAA1810.27066230018094No Hit
GAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCAT1800.2691669283567359No Hit
CAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATAT1750.2616900692357155No Hit
ATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAG1690.25271783829049094No Hit
GGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACT1670.24972709464208273No Hit
AAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAAT1670.24972709464208273No Hit
CCCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAG1660.24823172281787867No Hit
AGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTA1630.24374560734526637No Hit
AAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATC1630.24374560734526637No Hit
CTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGA1610.24075486369685822No Hit
CCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAAC1520.22729651727902145No Hit
ATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTT1490.22281040180640918No Hit
ATAAAACTTAAAACTTTACAGTCAGAGGTTCAATTCCTCTTCTTAACAAC1470.21981965815800097No Hit
CTCCCTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCT1450.21682891450959282No Hit
AATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCA1430.21383817086118462No Hit
ACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCAT1430.21383817086118462No Hit
AGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGG1420.21234279903698056No Hit
CAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCC1420.21234279903698056No Hit
CCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGA1410.21084742721277647No Hit
TTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCG1400.20935205538857235No Hit
GTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTTAC1380.2063613117401642No Hit
CACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATAC1370.2048659399159601No Hit
GAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCT1360.203370568091756No Hit
ATTACCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGG1360.203370568091756No Hit
TGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCC1330.19888445261914378No Hit
TGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAA1330.19888445261914378No Hit
ATCGCATAAAACTTAAAACTTTACAGTCAGAGGTTCAATTCCTCTTCTTA1320.19738908079493964No Hit
GATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTT1280.1914075934981233No Hit
GTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTT1260.18841684984971513No Hit
AAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAACCCT1250.18692147802551104No Hit
TTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTA1230.18393073437710286No Hit
TCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATA1230.18393073437710286No Hit
GGTAATCGCATAAAACTTAAAACTTTACAGTCAGAGGTTCAATTCCTCTT1220.18243536255289877No Hit
GATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGT1220.18243536255289877No Hit
CAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATT1200.1794446189044906No Hit
AAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCAC1200.1794446189044906No Hit
AACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGA1180.17645387525608242No Hit
AGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAA1180.17645387525608242No Hit
ACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGAT1170.17495850343187833No Hit
CCCTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTC1160.17346313160767424No Hit
GACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAAC1160.17346313160767424No Hit
ATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAA1150.17196775978347018No Hit
TTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTAT1130.168977016135062No Hit
TCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACCC1100.1644909006624497No Hit
AAATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAG1090.16299552883824564No Hit
TACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTAT1080.16150015701404152No Hit
AAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAACCC1080.16150015701404152No Hit
ATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGG1070.16000478518983746No Hit
TAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAA1060.15850941336563337No Hit
CGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAA1020.15252792606881702No Hit
TTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTTACAG1000.14953718242040884No Hit
CCTGTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCC980.14654643877200066No Hit
TAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGAT970.14505106694779657No Hit
AAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATGCGATT970.14505106694779657No Hit
GGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCT970.14505106694779657No Hit
ACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCAT960.14355569512359248No Hit
TCTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATAATACTA950.1420603232993884No Hit
ACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAAAC940.1405649514751843No Hit
GCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCC930.1390695796509802No Hit
GGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTT930.1390695796509802No Hit
TATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGG920.13757420782677612No Hit
CCGGGCTCTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATA900.13458346417836795No Hit
GCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTT900.13458346417836795No Hit
AGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAACCCTG890.13308809235416386No Hit
GGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGA860.12860197688155162No Hit
ACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAA810.12112511776053116No Hit
TTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACC800.11962974593632708No Hit
AACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGG800.11962974593632708No Hit
AGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATG790.11813437411212298No Hit
TATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTT780.1166390022879189No Hit
AAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCA780.1166390022879189No Hit
ATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTTACAGTCAGAGGT780.1166390022879189No Hit
TGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGAACAGGGT760.11364825863951072No Hit
CCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAAT760.11364825863951072No Hit
GAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAAC760.11364825863951072No Hit
GAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAA750.11215288681530662No Hit
GGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAG750.11215288681530662No Hit
AGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGAC740.11065751499110255No Hit
TCTGACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTT720.10766677134269435No Hit
GAGTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTC720.10766677134269435No Hit
ACTGTAAAGTTTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACA700.10467602769428618No Hit
AGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCA700.10467602769428618No Hit
GGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAG690.1031806558700821No Hit
TTTAAGTTTTATGCGATTACCGGGCTCTGCCATCTTAACAAACCCTGTTC680.101685284045878No Hit
CTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTTAAGATGGCA680.101685284045878No Hit
CACCCACCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCA670.10018991222167392No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)