FastQCFastQC Report
Tue 23 Aug 2022
EGAF00006144268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006144268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78794
Sequences flagged as poor quality0
Sequence length32-251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAACCCAGCTCTCCCTAAGCTTCAAACTAGACTACTTCTCCATAATA44555.653983805873543No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT43935.57529761149326TruSeq Adapter, Index 13 (97% over 38bp)
TCGATGATGTGGTCTTTGGAGTAGAAACCTGTGAGGAAAGGTATTCCTGC16192.054724979059319No Hit
CGATGATGTGGTCTTTGGAGTAGAAACCTGTGAGGAAAGGTATTCCTGCT7180.9112368962103714No Hit
ATCAACCAACCACACCTAGCATTCCTGCACATCTGTACCCACGCCTTCTT4100.5203441886438054No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3330.4226210117521638No Hit
ATCAAAAAAATCGTAGCCTTCTCCACTTCAAGTCAACTAGGACTCATAAT3050.3870853110642942No Hit
GTTGATGATACGCCCGAGCAGATGCCAACACAGCAGCCATTCAAGCAATC2430.30839911668401143No Hit
GATGAACAGTTGGAATAGGTTGTTAGCGGTAACTAAGATTAGTATGGTAA2250.285554737670381No Hit
ATCACCACTCTGTTCGCAGCAGTCTGCGCCCTTACACAAAATGACATCAA2210.2804782090006853No Hit
GTCTGCGCCCTTACACAAAATGACATCAAAAAAATCGTAGCCTTCTCCAC2190.2779399446658375No Hit
GTTGGAATAGGTTGTTAGCGGTAACTAAGATTAGTATGGTAATTAGGAAG2170.27540168033098966No Hit
GAAAATAGCCCACTAATCCAAACTCTAACACTATGCTTAGGCGCTATCAC2150.27286341599614183No Hit
ATCCAAACTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCAGC2110.2677868873264462No Hit
GTCAGGGGTGGAGACCTAATTGGGCTGATTTGCCTGCTGCTGCTAGGAGG2110.2677868873264462No Hit
ATCATCCACAACCTTAACAATGAACAAGATATTCGAAAAATAGGAGGACT2060.2614412264893266No Hit
ATTTTCTGCTAGGGGGTGGAAGCGGATGAGTAAGAAGATTCCTGCTACAA2020.25636469781963095No Hit
GATGCCAACACAGCAGCCATTCAAGCAATCCTATACAACCGTATCGGCGA1850.23478945097342435No Hit
ATCGCCGATACGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCAT1830.23225118663857655No Hit
ACTAATCCAAACTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCG1760.22336726146660912No Hit
GTTCTTCAAATATCTACTCATCTTCCTAATTACCATACTAATCTTAGTTA1750.2220981292991852No Hit
ATCATAGAATTCTCACTGTGATATATAAACTCAGACCCAAACATTAATCA1740.22082899713176132No Hit
GTTGACTTGAAGTGGAGAAGGCTACGATTTTTTTGATGTCATTTTGTGTA1690.21448333629464172No Hit
ATAATTCCTACGCCCTCTCAGCCGATGAACAGTTGGAATAGGTTGTTAGC1670.21194507195979392No Hit
GTTACCGCTAACAACCTATTCCAACTGTTCATCGGCTGAGAGGGCGTAGG1660.21067593979236998No Hit
GTTACAATCGGCATCAACCAACCACACCTAGCATTCCTGCACATCTGTAC1660.21067593979236998No Hit
GTCTCAGCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAATCTTCTT1650.20940680762494604No Hit
GTGGGGTGAGGCTTGGATTAGCGTTTAGAAGGGCTATTTGTTGTGGGTCT1630.2068685432900982No Hit
CTTCTTCAAAGCCATACTATTTATGTGCTCCGGGTCCATCATCCACAACC1610.2043302789552504No Hit
ACTAAGATTAGTATGGTAATTAGGAAGATGAGTAGATATTTGAAGAACTG1610.2043302789552504No Hit
CTCAGCCGATGAACAGTTGGAATAGGTTGTTAGCGGTAACTAAGATTAGT1560.1979846181181308No Hit
GATACGCCCGAGCAGATGCCAACACAGCAGCCATTCAAGCAATCCTATAC1540.19544635378328298No Hit
ATAATAGTTACAATCGGCATCAACCAACCACACCTAGCATTCCTGCACAT1520.19290808944843515No Hit
GTCATTTTGTGTAAGGGCGCAGACTGCTGCGAACAGAGTGGTGATAGCGC1480.1878315607787395No Hit
CTCTCAGCCGATGAACAGTTGGAATAGGTTGTTAGCGGTAACTAAGATTA1460.18529329644389167No Hit
GATTTGCCTGCTGCTGCTAGGAGGAGGCCTAGTAGTGGGGTGAGGCTTGG1440.18275503210904384No Hit
ATTGTTCGTTACATGGTCCATCATAGAATTCTCACTGTGATATATAAACT1420.180216767774196No Hit
GTTCGCAGCAGTCTGCGCCCTTACACAAAATGACATCAAAAAAATCGTAG1400.17767850343934816No Hit
ATGCTAGGCTGCCAATGGTGAGGGAGGTTGAAGTGAGAGGTATGGTTTTG1400.17767850343934816No Hit
ATCGGCGATATCGGTTTCATCCTCGCCTTAGCATGATTTATCCTACACTC1390.17640937127192424No Hit
ATTCCTGCACATCTGTACCCACGCCTTCTTCAAAGCCATACTATTTATGT1380.17514023910450036No Hit
ATTTGTTGTGGGTCTCATGAGTTGGAGTGTAGGATAAATCATGCTAAGGC1380.17514023910450036No Hit
GTAGGGCTGAGACTGGGGTGGGGCCTTCTATGGCTGAGGGGAGTCAGGGG1370.17387110693707644No Hit
ATCCGCTTCCACCCCCTAGCAGAAAATAGCCCACTAATCCAAACTCTAAC1370.17387110693707644No Hit
ATTAGGAAGATGAGTAGATATTTGAAGAACTGATTAATGTTTGGGTCTGA1350.17133284260222859No Hit
ATCCAAGCCTCACCCCACTACTAGGCCTCCTCCTAGCAGCAGCAGGCAAA1350.17133284260222859No Hit
ATCTTCCTAATTACCATACTAATCTTAGTTACCGCTAACAACCTATTCCA1340.17006371043480467No Hit
GTGAGGCTTGGATTAGCGTTTAGAAGGGCTATTTGTTGTGGGTCTCATGA1310.16625631393253293No Hit
ATCTTAGTTACCGCTAACAACCTATTCCAACTGTTCATCGGCTGAGAGGG1310.16625631393253293No Hit
CTTACACAAAATGACATCAAAAAAATCGTAGCCTTCTCCACTTCAAGTCA1300.16498718176510901No Hit
GTGGAGACCTAATTGGGCTGATTTGCCTGCTGCTGCTAGGAGGAGGCCTA1280.16244891743026119No Hit
ATCTGCTCGGGCGTATCATCAACTGATGAGCAAGAAGGATATAATTCCTA1280.16244891743026119No Hit
ATTTTTCGAATATCTTGTTCATTGTTAAGGTTGTGGATGATGGACCCGGA1280.16244891743026119No Hit
CTCATAATAGTTACAATCGGCATCAACCAACCACACCTAGCATTCCTGCA1270.16117978526283727No Hit
ATCTGTACCCACGCCTTCTTCAAAGCCATACTATTTATGTGCTCCGGGTC1270.16117978526283727No Hit
GACTGCTGCGAACAGAGTGGTGATAGCGCCTAAGCATAGTGTTAGAGTTT1260.15991065309541336No Hit
ATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTTAGCATGATTTA1260.15991065309541336No Hit
ATACTATTTATGTGCTCCGGGTCCATCATCCACAACCTTAACAATGAACA1260.15991065309541336No Hit
ATTTGAAGAACTGATTAATGTTTGGGTCTGAGTTTATATATCACAGTGAG1260.15991065309541336No Hit
ATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTTAGCATG1260.15991065309541336No Hit
ATATAAACTCAGACCCAAACATTAATCAGTTCTTCAAATATCTACTCATC1250.15864152092798944No Hit
CTTCTATGGCTGAGGGGAGTCAGGGGTGGAGACCTAATTGGGCTGATTTG1240.15737238876056553No Hit
ACTTCAAGTCAACTAGGACTCATAATAGTTACAATCGGCATCAACCAACC1240.15737238876056553No Hit
ACCAACCACACCTAGCATTCCTGCACATCTGTACCCACGCCTTCTTCAAA1240.15737238876056553No Hit
ATTGGGCTGATTTGCCTGCTGCTGCTAGGAGGAGGCCTAGTAGTGGGGTG1240.15737238876056553No Hit
GGTTGAAGTGAGAGGTATGGTTTTGAGTAGTCCTCCTATTTTTCGAATAT1230.15610325659314161No Hit
GTTAGAGTTTGGATTAGTGGGCTATTTTCTGCTAGGGGGTGGAAGCGGAT1220.1548341244257177No Hit
GTGGTTGGTTGATGCCGATTGTAACTATTATGAGTCCTAGTTGACTTGAA1220.1548341244257177No Hit
CTTCAAGTCAACTAGGACTCATAATAGTTACAATCGGCATCAACCAACCA1210.1535649922582938No Hit
GATATTTGAAGAACTGATTAATGTTTGGGTCTGAGTTTATATATCACAGT1200.15229586009086987No Hit
GTGCAGGAATGCTAGGTGTGGTTGGTTGATGCCGATTGTAACTATTATGA1200.15229586009086987No Hit
GTGTAGGATAAATCATGCTAAGGCGAGGATGAAACCGATATCGCCGATAC1180.14975759575602202No Hit
GTGTTGGCATCTGCTCGGGCGTATCATCAACTGATGAGCAAGAAGGATAT1180.14975759575602202No Hit
ATCATCAACTGATGAGCAAGAAGGATATAATTCCTACGCCCTCTCAGCCG1180.14975759575602202No Hit
GTAGAAACCTGTGAGGAAAGGTATTCCTGCTAATGCTAGGCTGCCAATGG1170.1484884635885981No Hit
GTGGGCTATTTTCTGCTAGGGGGTGGAAGCGGATGAGTAAGAAGATTCCT1160.14721933142117422No Hit
ATGCTAGGTGTGGTTGGTTGATGCCGATTGTAACTATTATGAGTCCTAGT1150.1459501992537503No Hit
ATAGGTTGTTAGCGGTAACTAAGATTAGTATGGTAATTAGGAAGATGAGT1150.1459501992537503No Hit
ATTCAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGC1150.1459501992537503No Hit
GTATGGTAATTAGGAAGATGAGTAGATATTTGAAGAACTGATTAATGTTT1140.1446810670863264No Hit
GTGGGTACAGATGTGCAGGAATGCTAGGTGTGGTTGGTTGATGCCGATTG1110.14087367058405462No Hit
GTCCTAGTTGACTTGAAGTGGAGAAGGCTACGATTTTTTTGATGTCATTT1110.14087367058405462No Hit
ACTGATGAGCAAGAAGGATATAATTCCTACGCCCTCTCAGCCGATGAACA1100.1396045384166307No Hit
GTAGTGGGGTGAGGCTTGGATTAGCGTTTAGAAGGGCTATTTGTTGTGGG1090.1383354062492068No Hit
ATCAACTGATGAGCAAGAAGGATATAATTCCTACGCCCTCTCAGCCGATG1080.13706627408178287No Hit
ATAGAAGGCCCCACCCCAGTCTCAGCCCTACTCCACTCAAGCACTATAGT1080.13706627408178287No Hit
ATCGGCATCAACCAACCACACCTAGCATTCCTGCACATCTGTACCCACGC1070.13579714191435896No Hit
ATCGGTTTCATCCTCGCCTTAGCATGATTTATCCTACACTCCAACTCATG1060.13452800974693505No Hit
GGTGTGGTTGGTTGATGCCGATTGTAACTATTATGAGTCCTAGTTGACTT1060.13452800974693505No Hit
GTAGGAATTATATCCTTCTTGCTCATCAGTTGATGATACGCCCGAGCAGA1050.13325887757951113No Hit
GTTAGCGGTAACTAAGATTAGTATGGTAATTAGGAAGATGAGTAGATATT1040.13198974541208722No Hit
GATACGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATCTGCTC1040.13198974541208722No Hit
GTAAGAAGATTCCTGCTACAACTATAGTGCTTGAGTGGAGTAGGGCTGAG1040.13198974541208722No Hit
ACAACAAATAGCCCTTCTAAACGCTAATCCAAGCCTCACCCCACTACTAG1020.12945148107723936No Hit
ACGCCTTCTTCAAAGCCATACTATTTATGTGCTCCGGGTCCATCATCCAC1010.12818234890981547No Hit
ATTCCAACTGTTCATCGGCTGAGAGGGCGTAGGAATTATATCCTTCTTGC1000.12691321674239156No Hit
GTGGAGAAGGCTACGATTTTTTTGATGTCATTTTGTGTAAGGGCGCAGAC980.12437495240754373No Hit
GTCCTCCTATTTTTCGAATATCTTGTTCATTGTTAAGGTTGTGGATGATG980.12437495240754373No Hit
ATTTATGTGCTCCGGGTCCATCATCCACAACCTTAACAATGAACAAGATA970.12310582024011982No Hit
ACCACACCTAGCATTCCTGCACATCTGTACCCACGCCTTCTTCAAAGCCA970.12310582024011982No Hit
ATCAGTTGATGATACGCCCGAGCAGATGCCAACACAGCAGCCATTCAAGC970.12310582024011982No Hit
CTTCTCCATAATATTCATCCCTGTAGCATTGTTCGTTACATGGTCCATCA970.12310582024011982No Hit
GGTAACTAAGATTAGTATGGTAATTAGGAAGATGAGTAGATATTTGAAGA960.12183668807269589No Hit
GTTGGCATCTGCTCGGGCGTATCATCAACTGATGAGCAAGAAGGATATAA950.12056755590527198No Hit
GTTGTAGCAGGAATCTTCTTACTCATCCGCTTCCACCCCCTAGCAGAAAA930.11802929157042415No Hit
GTTGGAGTGTAGGATAAATCATGCTAAGGCGAGGATGAAACCGATATCGC930.11802929157042415No Hit
CTCATGAGTTGGAGTGTAGGATAAATCATGCTAAGGCGAGGATGAAACCG930.11802929157042415No Hit
ATCTTCTTACTCATCCGCTTCCACCCCCTAGCAGAAAATAGCCCACTAAT930.11802929157042415No Hit
GTTTGGATTAGTGGGCTATTTTCTGCTAGGGGGTGGAAGCGGATGAGTAA930.11802929157042415No Hit
CTTCTAAACGCTAATCCAAGCCTCACCCCACTACTAGGCCTCCTCCTAGC920.11676015940300023No Hit
GTTTAGAAGGGCTATTTGTTGTGGGTCTCATGAGTTGGAGTGTAGGATAA910.1154910272355763No Hit
ATCGGCTGAGAGGGCGTAGGAATTATATCCTTCTTGCTCATCAGTTGATG910.1154910272355763No Hit
GTTCATCGGCTGAGAGGGCGTAGGAATTATATCCTTCTTGCTCATCAGTT900.11422189506815239No Hit
CTGCACATCTGTACCCACGCCTTCTTCAAAGCCATACTATTTATGTGCTC900.11422189506815239No Hit
ACCTAGCATTCCTGCACATCTGTACCCACGCCTTCTTCAAAGCCATACTA900.11422189506815239No Hit
GTTGATGCCGATTGTAACTATTATGAGTCCTAGTTGACTTGAAGTGGAGA890.11295276290072848No Hit
ATAAATAGTATGGCTTTGAAGAAGGCGTGGGTACAGATGTGCAGGAATGC890.11295276290072848No Hit
ATTCCTGCTAATGCTAGGCTGCCAATGGTGAGGGAGGTTGAAGTGAGAGG890.11295276290072848No Hit
ATGGTAATTAGGAAGATGAGTAGATATTTGAAGAACTGATTAATGTTTGG890.11295276290072848No Hit
GGTTGTTAGCGGTAACTAAGATTAGTATGGTAATTAGGAAGATGAGTAGA880.11168363073330456No Hit
CTATAGTTGTAGCAGGAATCTTCTTACTCATCCGCTTCCACCCCCTAGCA880.11168363073330456No Hit
GCTATCACCACTCTGTTCGCAGCAGTCTGCGCCCTTACACAAAATGACAT870.11041449856588066No Hit
CTATTTATGTGCTCCGGGTCCATCATCCACAACCTTAACAATGAACAAGA870.11041449856588066No Hit
GGCAAATCAGCCCAATTAGGTCTCCACCCCTGACTCCCCTCAGCCATAGA870.11041449856588066No Hit
ACTAGGACTCATAATAGTTACAATCGGCATCAACCAACCACACCTAGCAT870.11041449856588066No Hit
GGATAAATCATGCTAAGGCGAGGATGAAACCGATATCGCCGATACGGTTG860.10914536639845675No Hit
GTGCTTGAGTGGAGTAGGGCTGAGACTGGGGTGGGGCCTTCTATGGCTGA860.10914536639845675No Hit
GTACCCACGCCTTCTTCAAAGCCATACTATTTATGTGCTCCGGGTCCATC850.10787623423103282No Hit
AAACATTAATCAGTTCTTCAAATATCTACTCATCTTCCTAATTACCATAC850.10787623423103282No Hit
GGTATGGTTTTGAGTAGTCCTCCTATTTTTCGAATATCTTGTTCATTGTT840.1066071020636089No Hit
ATGGTGAGGGAGGTTGAAGTGAGAGGTATGGTTTTGAGTAGTCCTCCTAT840.1066071020636089No Hit
ACTACTAGGCCTCCTCCTAGCAGCAGCAGGCAAATCAGCCCAATTAGGTC830.10533796989618499No Hit
ATGATTTATCCTACACTCCAACTCATGAGACCCACAACAAATAGCCCTTC830.10533796989618499No Hit
GATGAAACCGATATCGCCGATACGGTTGTATAGGATTGCTTGAATGGCTG830.10533796989618499No Hit
GATAGCGCCTAAGCATAGTGTTAGAGTTTGGATTAGTGGGCTATTTTCTG830.10533796989618499No Hit
AATTAGGAAGATGAGTAGATATTTGAAGAACTGATTAATGTTTGGGTCTG820.10406883772876108No Hit
TTCTAAACGCTAATCCAAGCCTCACCCCACTACTAGGCCTCCTCCTAGCA820.10406883772876108No Hit
ATGCTTAGGCGCTATCACCACTCTGTTCGCAGCAGTCTGCGCCCTTACAC810.10279970556133716No Hit
CATCTGTACCCACGCCTTCTTCAAAGCCATACTATTTATGTGCTCCGGGT810.10279970556133716No Hit
GTGGGGCCTTCTATGGCTGAGGGGAGTCAGGGGTGGAGACCTAATTGGGC800.10153057339391323No Hit
CCTTACACAAAATGACATCAAAAAAATCGTAGCCTTCTCCACTTCAAGTC800.10153057339391323No Hit
CTGTGATATATAAACTCAGACCCAAACATTAATCAGTTCTTCAAATATCT800.10153057339391323No Hit
ATTACCATACTAATCTTAGTTACCGCTAACAACCTATTCCAACTGTTCAT800.10153057339391323No Hit
GGCTTGGATTAGCGTTTAGAAGGGCTATTTGTTGTGGGTCTCATGAGTTG800.10153057339391323No Hit
CTCAGCCATAGAAGGCCCCACCCCAGTCTCAGCCCTACTCCACTCAAGCA790.10026144122648932No Hit
CTCTGTTCGCAGCAGTCTGCGCCCTTACACAAAATGACATCAAAAAAATC790.10026144122648932No Hit
GGCTACGATTTTTTTGATGTCATTTTGTGTAAGGGCGCAGACTGCTGCGA790.10026144122648932No Hit
CTGCTAATGCTAGGCTGCCAATGGTGAGGGAGGTTGAAGTGAGAGGTATG790.10026144122648932No Hit
GTGAGGAAAGGTATTCCTGCTAATGCTAGGCTGCCAATGGTGAGGGAGGT790.10026144122648932No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)