FastQCFastQC Report
Tue 23 Aug 2022
EGAF00006144269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006144269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78794
Sequences flagged as poor quality0
Sequence length32-251
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAACCCAGCTCTCCCTAAGCTTCAAACTAGACTACTTCTCCATAATA40285.112064370383532No Hit
CGATGATGTGGTCTTTGGAGTAGAAACCTGTGAGGAAAGGTATTCCTGCT16942.1499098916161126No Hit
TCGATGATGTGGTCTTTGGAGTAGAAACCTGTGAGGAAAGGTATTCCTGC15441.9595400665025255No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3250.41246795441277256No Hit
ATCAACCAACCACACCTAGCATTCCTGCACATCTGTACCCACGCCTTCTT2940.37312485722263117No Hit
GATGAACAGTTGGAATAGGTTGTTAGCGGTAACTAAGATTAGTATGGTAA2060.2614412264893266No Hit
GTTGATGATACGCCCGAGCAGATGCCAACACAGCAGCCATTCAAGCAATC1960.24874990481508746No Hit
ATTTTCTGCTAGGGGGTGGAAGCGGATGAGTAAGAAGATTCCTGCTACAA1930.2449425083128157No Hit
ATCAAAAAAATCGTAGCCTTCTCCACTTCAAGTCAACTAGGACTCATAAT1900.24113511181054395No Hit
GTTGGAATAGGTTGTTAGCGGTAACTAAGATTAGTATGGTAATTAGGAAG1890.23986597964312006No Hit
GTCAGGGGTGGAGACCTAATTGGGCTGATTTGCCTGCTGCTGCTAGGAGG1760.22336726146660912No Hit
CTCAGCCGATGAACAGTTGGAATAGGTTGTTAGCGGTAACTAAGATTAGT1760.22336726146660912No Hit
GTTGACTTGAAGTGGAGAAGGCTACGATTTTTTTGATGTCATTTTGTGTA1720.2182907327969135No Hit
CTCTCAGCCGATGAACAGTTGGAATAGGTTGTTAGCGGTAACTAAGATTA1700.21575246846206564No Hit
GTTCTTCAAATATCTACTCATCTTCCTAATTACCATACTAATCTTAGTTA1700.21575246846206564No Hit
ATCGCCGATACGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCAT1610.2043302789552504No Hit
ATCATAGAATTCTCACTGTGATATATAAACTCAGACCCAAACATTAATCA1610.2043302789552504No Hit
ATCCAAACTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCAGC1580.20052288245297864No Hit
GAAAATAGCCCACTAATCCAAACTCTAACACTATGCTTAGGCGCTATCAC1560.1979846181181308No Hit
GTGGTTGGTTGATGCCGATTGTAACTATTATGAGTCCTAGTTGACTTGAA1540.19544635378328298No Hit
GTCTCAGCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAATCTTCTT1530.1941772216158591No Hit
GTCTGCGCCCTTACACAAAATGACATCAAAAAAATCGTAGCCTTCTCCAC1520.19290808944843515No Hit
ATCACCACTCTGTTCGCAGCAGTCTGCGCCCTTACACAAAATGACATCAA1520.19290808944843515No Hit
ATTAGGAAGATGAGTAGATATTTGAAGAACTGATTAATGTTTGGGTCTGA1500.19036982511358733No Hit
ACTAATCCAAACTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCG1460.18529329644389167No Hit
GTCATTTTGTGTAAGGGCGCAGACTGCTGCGAACAGAGTGGTGATAGCGC1450.18402416427646776No Hit
ATTTTTCGAATATCTTGTTCATTGTTAAGGTTGTGGATGATGGACCCGGA1420.180216767774196No Hit
GTGCAGGAATGCTAGGTGTGGTTGGTTGATGCCGATTGTAACTATTATGA1410.17894763560677207No Hit
GATGCCAACACAGCAGCCATTCAAGCAATCCTATACAACCGTATCGGCGA1400.17767850343934816No Hit
ATGCTAGGCTGCCAATGGTGAGGGAGGTTGAAGTGAGAGGTATGGTTTTG1370.17387110693707644No Hit
GTAGAAACCTGTGAGGAAAGGTATTCCTGCTAATGCTAGGCTGCCAATGG1330.16879457826738076No Hit
ATCTTCCTAATTACCATACTAATCTTAGTTACCGCTAACAACCTATTCCA1330.16879457826738076No Hit
ATCATCCACAACCTTAACAATGAACAAGATATTCGAAAAATAGGAGGACT1320.16752544609995684No Hit
GTGGAGACCTAATTGGGCTGATTTGCCTGCTGCTGCTAGGAGGAGGCCTA1320.16752544609995684No Hit
ATTTGTTGTGGGTCTCATGAGTTGGAGTGTAGGATAAATCATGCTAAGGC1310.16625631393253293No Hit
GTTACAATCGGCATCAACCAACCACACCTAGCATTCCTGCACATCTGTAC1300.16498718176510901No Hit
GGTGTGGTTGGTTGATGCCGATTGTAACTATTATGAGTCCTAGTTGACTT1290.1637180495976851No Hit
ACTAAGATTAGTATGGTAATTAGGAAGATGAGTAGATATTTGAAGAACTG1280.16244891743026119No Hit
ATCATCAACTGATGAGCAAGAAGGATATAATTCCTACGCCCTCTCAGCCG1280.16244891743026119No Hit
GTGGGGTGAGGCTTGGATTAGCGTTTAGAAGGGCTATTTGTTGTGGGTCT1260.15991065309541336No Hit
ATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTTAGCATGATTTA1260.15991065309541336No Hit
ATCTGCTCGGGCGTATCATCAACTGATGAGCAAGAAGGATATAATTCCTA1250.15864152092798944No Hit
GATTTGCCTGCTGCTGCTAGGAGGAGGCCTAGTAGTGGGGTGAGGCTTGG1240.15737238876056553No Hit
GATACGCCCGAGCAGATGCCAACACAGCAGCCATTCAAGCAATCCTATAC1240.15737238876056553No Hit
GTTACCGCTAACAACCTATTCCAACTGTTCATCGGCTGAGAGGGCGTAGG1230.15610325659314161No Hit
GTAGGGCTGAGACTGGGGTGGGGCCTTCTATGGCTGAGGGGAGTCAGGGG1230.15610325659314161No Hit
ATGCTAGGTGTGGTTGGTTGATGCCGATTGTAACTATTATGAGTCCTAGT1210.1535649922582938No Hit
CTTCTATGGCTGAGGGGAGTCAGGGGTGGAGACCTAATTGGGCTGATTTG1210.1535649922582938No Hit
GTGAGGCTTGGATTAGCGTTTAGAAGGGCTATTTGTTGTGGGTCTCATGA1200.15229586009086987No Hit
ATATAAACTCAGACCCAAACATTAATCAGTTCTTCAAATATCTACTCATC1200.15229586009086987No Hit
ATTGTTCGTTACATGGTCCATCATAGAATTCTCACTGTGATATATAAACT1190.15102672792344593No Hit
ATTCCTGCACATCTGTACCCACGCCTTCTTCAAAGCCATACTATTTATGT1180.14975759575602202No Hit
GTGGGTACAGATGTGCAGGAATGCTAGGTGTGGTTGGTTGATGCCGATTG1180.14975759575602202No Hit
ATAATAGTTACAATCGGCATCAACCAACCACACCTAGCATTCCTGCACAT1160.14721933142117422No Hit
GTTAGCGGTAACTAAGATTAGTATGGTAATTAGGAAGATGAGTAGATATT1150.1459501992537503No Hit
ATCCGCTTCCACCCCCTAGCAGAAAATAGCCCACTAATCCAAACTCTAAC1150.1459501992537503No Hit
CTCATGAGTTGGAGTGTAGGATAAATCATGCTAAGGCGAGGATGAAACCG1140.1446810670863264No Hit
ATCCAAGCCTCACCCCACTACTAGGCCTCCTCCTAGCAGCAGCAGGCAAA1140.1446810670863264No Hit
CTTCAAGTCAACTAGGACTCATAATAGTTACAATCGGCATCAACCAACCA1140.1446810670863264No Hit
GTGTAGGATAAATCATGCTAAGGCGAGGATGAAACCGATATCGCCGATAC1130.14341193491890245No Hit
GTTAGAGTTTGGATTAGTGGGCTATTTTCTGCTAGGGGGTGGAAGCGGAT1130.14341193491890245No Hit
GTAGTGGGGTGAGGCTTGGATTAGCGTTTAGAAGGGCTATTTGTTGTGGG1130.14341193491890245No Hit
GGTTGAAGTGAGAGGTATGGTTTTGAGTAGTCCTCCTATTTTTCGAATAT1110.14087367058405462No Hit
GTCCTCCTATTTTTCGAATATCTTGTTCATTGTTAAGGTTGTGGATGATG1100.1396045384166307No Hit
ACTTCAAGTCAACTAGGACTCATAATAGTTACAATCGGCATCAACCAACC1100.1396045384166307No Hit
ATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTTAGCATG1100.1396045384166307No Hit
GTGAGAGGTATGGTTTTGAGTAGTCCTCCTATTTTTCGAATATCTTGTTC1090.1383354062492068No Hit
GATACGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATCTGCTC1080.13706627408178287No Hit
ATTCAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGC1080.13706627408178287No Hit
GATATTTGAAGAACTGATTAATGTTTGGGTCTGAGTTTATATATCACAGT1070.13579714191435896No Hit
ATTTGAAGAACTGATTAATGTTTGGGTCTGAGTTTATATATCACAGTGAG1070.13579714191435896No Hit
ATCGGTTTCATCCTCGCCTTAGCATGATTTATCCTACACTCCAACTCATG1060.13452800974693505No Hit
CTGCTAATGCTAGGCTGCCAATGGTGAGGGAGGTTGAAGTGAGAGGTATG1060.13452800974693505No Hit
GGTAACTAAGATTAGTATGGTAATTAGGAAGATGAGTAGATATTTGAAGA1060.13452800974693505No Hit
ATTGGGCTGATTTGCCTGCTGCTGCTAGGAGGAGGCCTAGTAGTGGGGTG1060.13452800974693505No Hit
GTGTTGGCATCTGCTCGGGCGTATCATCAACTGATGAGCAAGAAGGATAT1050.13325887757951113No Hit
ATAGAAGGCCCCACCCCAGTCTCAGCCCTACTCCACTCAAGCACTATAGT1050.13325887757951113No Hit
GTCCTAGTTGACTTGAAGTGGAGAAGGCTACGATTTTTTTGATGTCATTT1040.13198974541208722No Hit
ATAAATAGTATGGCTTTGAAGAAGGCGTGGGTACAGATGTGCAGGAATGC1030.1307206132446633No Hit
ACAACAAATAGCCCTTCTAAACGCTAATCCAAGCCTCACCCCACTACTAG1020.12945148107723936No Hit
GACTGCTGCGAACAGAGTGGTGATAGCGCCTAAGCATAGTGTTAGAGTTT1020.12945148107723936No Hit
ATAATTCCTACGCCCTCTCAGCCGATGAACAGTTGGAATAGGTTGTTAGC1010.12818234890981547No Hit
ATCTTAGTTACCGCTAACAACCTATTCCAACTGTTCATCGGCTGAGAGGG1010.12818234890981547No Hit
CTTCTTCAAAGCCATACTATTTATGTGCTCCGGGTCCATCATCCACAACC1000.12691321674239156No Hit
ATTCCTGCTAATGCTAGGCTGCCAATGGTGAGGGAGGTTGAAGTGAGAGG1000.12691321674239156No Hit
GTTCGCAGCAGTCTGCGCCCTTACACAAAATGACATCAAAAAAATCGTAG1000.12691321674239156No Hit
GTGGAGAAGGCTACGATTTTTTTGATGTCATTTTGTGTAAGGGCGCAGAC990.12564408457496765No Hit
CTTCTAAACGCTAATCCAAGCCTCACCCCACTACTAGGCCTCCTCCTAGC980.12437495240754373No Hit
GTGGGCTATTTTCTGCTAGGGGGTGGAAGCGGATGAGTAAGAAGATTCCT970.12310582024011982No Hit
ACCCCAGTCTCAGCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAAT960.12183668807269589No Hit
ATACTATTTATGTGCTCCGGGTCCATCATCCACAACCTTAACAATGAACA960.12183668807269589No Hit
ATCTGTACCCACGCCTTCTTCAAAGCCATACTATTTATGTGCTCCGGGTC960.12183668807269589No Hit
ATTCCAACTGTTCATCGGCTGAGAGGGCGTAGGAATTATATCCTTCTTGC950.12056755590527198No Hit
CCTCTCAGCCGATGAACAGTTGGAATAGGTTGTTAGCGGTAACTAAGATT940.11929842373784806No Hit
ATCGGCGATATCGGTTTCATCCTCGCCTTAGCATGATTTATCCTACACTC940.11929842373784806No Hit
GATAGCGCCTAAGCATAGTGTTAGAGTTTGGATTAGTGGGCTATTTTCTG940.11929842373784806No Hit
ATGGTAATTAGGAAGATGAGTAGATATTTGAAGAACTGATTAATGTTTGG930.11802929157042415No Hit
ATCAACTGATGAGCAAGAAGGATATAATTCCTACGCCCTCTCAGCCGATG930.11802929157042415No Hit
ACTGATGAGCAAGAAGGATATAATTCCTACGCCCTCTCAGCCGATGAACA910.1154910272355763No Hit
AACAACCCAGCTCTCCCTAAGCTTCAAACTAGACTACTTCTCCATAATAT910.1154910272355763No Hit
ATAGGTTGTTAGCGGTAACTAAGATTAGTATGGTAATTAGGAAGATGAGT910.1154910272355763No Hit
GTGGAAGCGGATGAGTAAGAAGATTCCTGCTACAACTATAGTGCTTGAGT910.1154910272355763No Hit
CTGTAGCATTGTTCGTTACATGGTCCATCATAGAATTCTCACTGTGATAT900.11422189506815239No Hit
ATCTTCTTACTCATCCGCTTCCACCCCCTAGCAGAAAATAGCCCACTAAT900.11422189506815239No Hit
GTTGGCATCTGCTCGGGCGTATCATCAACTGATGAGCAAGAAGGATATAA900.11422189506815239No Hit
CTTTGGAGTAGAAACCTGTGAGGAAAGGTATTCCTGCTAATGCTAGGCTG900.11422189506815239No Hit
GTTCATCGGCTGAGAGGGCGTAGGAATTATATCCTTCTTGCTCATCAGTT890.11295276290072848No Hit
CTTACACAAAATGACATCAAAAAAATCGTAGCCTTCTCCACTTCAAGTCA890.11295276290072848No Hit
CTGCACATCTGTACCCACGCCTTCTTCAAAGCCATACTATTTATGTGCTC880.11168363073330456No Hit
ATGGTGAGGGAGGTTGAAGTGAGAGGTATGGTTTTGAGTAGTCCTCCTAT880.11168363073330456No Hit
GTTGTAGCAGGAATCTTCTTACTCATCCGCTTCCACCCCCTAGCAGAAAA870.11041449856588066No Hit
GTAGGAATTATATCCTTCTTGCTCATCAGTTGATGATACGCCCGAGCAGA860.10914536639845675No Hit
GTTTGGATTAGTGGGCTATTTTCTGCTAGGGGGTGGAAGCGGATGAGTAA860.10914536639845675No Hit
GTTTAGAAGGGCTATTTGTTGTGGGTCTCATGAGTTGGAGTGTAGGATAA850.10787623423103282No Hit
CTCATAATAGTTACAATCGGCATCAACCAACCACACCTAGCATTCCTGCA850.10787623423103282No Hit
CTTCTCCATAATATTCATCCCTGTAGCATTGTTCGTTACATGGTCCATCA850.10787623423103282No Hit
GGTATGGTTTTGAGTAGTCCTCCTATTTTTCGAATATCTTGTTCATTGTT840.1066071020636089No Hit
CTCATCTTCCTAATTACCATACTAATCTTAGTTACCGCTAACAACCTATT840.1066071020636089No Hit
GTATGGCTTTGAAGAAGGCGTGGGTACAGATGTGCAGGAATGCTAGGTGT840.1066071020636089No Hit
GGCTACGATTTTTTTGATGTCATTTTGTGTAAGGGCGCAGACTGCTGCGA840.1066071020636089No Hit
GTAGGATAAATCATGCTAAGGCGAGGATGAAACCGATATCGCCGATACGG840.1066071020636089No Hit
ACTCAAGCACTATAGTTGTAGCAGGAATCTTCTTACTCATCCGCTTCCAC830.10533796989618499No Hit
GTATGGTAATTAGGAAGATGAGTAGATATTTGAAGAACTGATTAATGTTT820.10406883772876108No Hit
GTTGGAGTGTAGGATAAATCATGCTAAGGCGAGGATGAAACCGATATCGC820.10406883772876108No Hit
AACACAGCAGCCATTCAAGCAATCCTATACAACCGTATCGGCGATATCGG800.10153057339391323No Hit
AATTAGGAAGATGAGTAGATATTTGAAGAACTGATTAATGTTTGGGTCTG800.10153057339391323No Hit
TGTTAGCGGTAACTAAGATTAGTATGGTAATTAGGAAGATGAGTAGATAT800.10153057339391323No Hit
GTGAGGAAAGGTATTCCTGCTAATGCTAGGCTGCCAATGGTGAGGGAGGT800.10153057339391323No Hit
GTAAGAAGATTCCTGCTACAACTATAGTGCTTGAGTGGAGTAGGGCTGAG790.10026144122648932No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)