FastQCFastQC Report
Tue 28 Jun 2022
EGAF00006144270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006144270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2672611282
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC27679593610.356759992155117TruSeq Adapter, Index 8 (100% over 50bp)
GAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCT134960140.5049748196041627TruSeq Adapter, Index 8 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATGC37376380.13984966782011812TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCCCGTATGC29104770.10890012399491172TruSeq Adapter, Index 8 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)