FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006144282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006144282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2859864083
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCACGCAATCTCGTAT2471178658.640895435169533TruSeq Adapter, Index 1 (97% over 39bp)
GAAGAGCACACGTCTGAACTCCAGTCACTTCACGCAATCTCGTATGCCGT135274410.4730099266049631TruSeq Adapter, Index 1 (97% over 34bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTTCACGCAATCTCGTATGCC75241160.26309348212475875TruSeq Adapter, Index 1 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCACGCAATCTCGTATG52312340.18291897265664564TruSeq Adapter, Index 1 (97% over 38bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCACGCAATCTCGTAT29250370.10227888162194175TruSeq Adapter, Index 3 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)